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Intracellular Expression Of An Ice Nucleation Protein Reduces Cryoinjury In Insect Cells, Avril M. Harder
Intracellular Expression Of An Ice Nucleation Protein Reduces Cryoinjury In Insect Cells, Avril M. Harder
Student Honors Theses
Exposure of insect cells to subzero temperatures typically leads to cell membrane disruption and lethal intracellular ice formation. This study seeks to examine the cryoprotective value of trangenically expressing a bacterial ice nucleation protein (INP) in Spodoptera frugiperda (Sf-21) cells. The bacterium Pseudomonas syringae naturally produces a membrane-bound INP (inaZ), capable of structuring water and initiating ice formation at temperatures as high as -2 °C. I hypothesized that intracellular expression of an altered form of inaZ (PsINP) in Sf-21 cells will mediate highly regulated ice nucleation when cells are cooled to -80 °C in a slow, controlled manner, …