Life Sciences Commons^™

The Impact Of Temperature On The Early Stages Of The Development Of The Body Axis In The Model Species Astyanax Mexicanus (Teleostei: Characidae), Sara Mohammad Alharbi

College of Science and Health Theses and Dissertations

Environmental fluctuations can change the phenotypic traits of ectotherms. Ectotherms such as fishes are very susceptible to changes in temperature. Recent studies on adults of the Mexican tetra, Astyanax mexicanus, indicate that variation in temperature during development significantly impacts vertebral number and body shape. Since vertebral number and somite number are related, I examined whether temperature significantly impacts somitogenesis and the early development of the body axis in this species. Fertilized eggs of the surface form of lab-reared Astyanax mexicanus were subjected to temperature treatments of 20°C, 24°C, and 28°C, and fixed hourly as they developed until they hatched. Embryos …

Songbird Use Of Problem-Solving Feeders In Urban And Rural Areas, Kayce Miller

College of Science and Health Theses and Dissertations

Behavioral flexibility is important for animals to adapt to novel situations in their environment. It has been suggested that birds living in complex environments (e.g., urban areas) should be more flexible than conspecifics in less complex environments. Birds are a particularly well studied group, where novel foraging problems are used to assess flexibility and problem-solving performance of urban and rural animals of the same species; however, this is most frequently done in a lab setting with wild-caught birds originating from different habitats. Using a field-based method to test problem-solving performance should give additional insight into other factors influencing birds’ flexibility. …

Developing A Transfection Method For Didymium Iridis, Uriel Adolfo Baray

College of Science and Health Theses and Dissertations

The plasmodial slime mold and member of Mycetozoa, Didymium iridis, has been studied in a variety of contexts such as RNA editing (Traphagen et al., 2010; Hendrickson and Silliker, 2010a; Hendrickson and Silliker, 2010b), mitochondrial inheritance (Silliker et al., 2002), biological speciation (Betterley and Collins, 1983; Clark et al., 1991) and mating competency (Shipley and Holt, 1982). Further studies are hindered by the lack of a transfection protocol, which would allow for gene manipulation in D. iridis (hereafter Didymium). Transfection methods developed in the related slime molds, Physarum polycephalum (Burland et al., 1993) and Dictyostelium discoideum (Fey et al., 1995; …

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Zygote Gene Expression And Plasmodial Development In Didymium Iridis, Sean Schaefer

College of Science and Health Theses and Dissertations

Didymium iridis is a cosmopolitan species of plasmodial slime mold consisting of two distinct life stages. Haploid amoebae and diploid plasmodia feed on microscopic organisms such as bacteria and fungi through phagocytosis. Sexually compatible haploid amoebae act as gametes which when fused embark on an irreversible developmental change resulting in a diploid zygote. The zygote can undergo closed mitosis resulting in a multinucleated plasmodium. Little is known about changes in gene expression during this developmental transition. Our principal goal in this study was to provide a comprehensive list of genes likely to be involved in plasmodial development. We performed suppressive …

Comparative Brain Anatomy Of Lamniform Sharks (Elasmobranchii: Lamniformes) And Its Implications To Function, Behavioral Ecology, And Evolution, Francesco Guzzo

College of Science and Health Theses and Dissertations

Understanding the diversity of brain morphology is important to understand the evolution of cognitive ability and how ecology and phylogeny have influenced the variation in brain complexity. I examined the morphological variation of the brain in the shark order Lamniformes based on museum specimens and literature. Where I illustrate a wide range of morphological diversity in lamniform brains, my study shows that there is a strong positive correlation between brain size and body size that sharks with a larger brain tend to have a more foliated cerebellum, but that the body weight over brain weight did not correlate with cerebellar …

Integument-Based Inferences On The Swimming Ability And Prey Hunting Strategy Of The Bigeye Thresher Shark, Alopias Superciliosus (Lamniformes: Alopiidae), Joseph Aaron Frumkin

College of Science and Health Theses and Dissertations

Alopias is a group of lamniform sharks characterized by a highly elongate caudal fin with three known extant species: A. pelagicus (pelagic thresher shark), A. superciliosus (bigeye thresher shark), and A. vulpinus (common thresher shark). Alopias pelagicus and A. vulpinus are considered fast swimmers and use their caudal fin to hunt for small schooling fish by stunning them, but the feeding behavior has never been directly observed for A. superciliosus. Under the ecomorphological framework, I examined the following four integumentary variables of selected fast swimming (e.g., A. pelagicus, A. vulpinus, and Lamna) and slow swimming (e.g., Mitsukurina and Megachasma) lamniform …

A New Pachyrhizodontid Fish (Actinopterygii: Teleostei) From The Tarrant Formation (Cenomanian) Of The Upper Cretaceous Eagle Ford Group Of Texas, U.S.A., Maxwell G. London

College of Science and Health Theses and Dissertations

SMU 76938 is a fossil skeleton of a large, nearly complete actinopterygian bony fish housed in Southern Methodist University in Dallas, Texas, USA. It was collected from the Upper Cretaceous Tarrant Formation (middle Cenomanian; ~96 Ma) of the Eagle Ford Group in Tarrant County, Texas, an area where it was near the western margin of the East Texas Embayment during the Late Cretaceous. Although parts of the skull and dorsal fin are damaged, SMU 76938 is relatively complete, especially in the preservation of the caudal fin with its soft tissue outline. The fish has a fusiform body and measures about …

Effect Of Promoting Psychosocial Resilience In Adolescents With Diabetes: An Integrative Literature Review, Britney Sandino

Grace Peterson Nursing Research Colloquium

Background: Adolescent obesity and diabetes increase the risk of numerous physiological health threats and psychosocial stress disorders. Educating this population on how to cope with physical and psychosocial stressors improves their health status and health-related quality of life (HRQOL).

Objectives: The purpose of this review was to provide the nursing community with insight on the health promotion effects of fostering psychosocial resilience in diabetic adolescents aged 7 to 17 and suggest specific methods to integrate into their plan of care.

Methods: An integrative literature review was performed using PubMed, Cumulative Index to Nursing and Health Literature, Ovid Medline, ProQuest …

Sex-Specific Personalities In The Purple Marsh Crab, Jillian Sterman, Jessica Barton, Panagiota Delmedico, Samantha Sweeney

DePaul Discoveries

Animals are considered to possess personalities when individuals differ in behavior, and these differences are consistent between situations. Several studies have identified personalities in diverse groups but less is known about personality variation between the sexes. In this study, we examined variation in two key personality traits (boldness, activity) in female and male purple marsh crabs (Sesarma reticulatum) using a semi-field approach. Specifically, we measured boldness and activity on two consecutive days using the same behavioral assays during each time point. Consistency (personality) was determined using Kendall’s coefficient of concordance based on Spearman correlation coefficients for each behavior. …

Behavioral Responses To Gold Nanoparticle Exposure And H2o2-Induced Oxidative Stress In Caenorhabditis Elegans, Rachel Pride, Ashley Wagner

DePaul Discoveries

Gold nanoparticles (AuNPs) have been utilized in many biomedical disciplines, most notably cancer therapy and drug delivery. Recent research suggests that with specific peptide manipulation, AuNPs can deliver drugs across the blood-brain barrier (BBB), allowing for treatment of neurodegeneration and other neurological afflictions. Neurodegeneration has been shown to be caused by oxidative stress. The present experiment aimed to assess the effects of AuNPs on C. elegans behavior that had undergone H₂O₂-induced oxidative stress. It was predicted that worms exposed to both H₂O₂ and AuNPs would have higher survival, mechanosensation, and thrashing rates than …

Effects Of Reduced Ph On Health Biomarkers Of The Seagrass Cymodocea Nodosa, Regan A. Mcenaney

DePaul Discoveries

Ocean acidification is a growing problem that may affect many marine organisms in the future. Within 100 years the pH of the ocean is predicted to decrease to 7.8, from the current ocean pH of around 8.1. Using phenolic acid levels as a stress indicator as well as respiration and chlorophyll content as a measure of health, the effect of lowering pH was tested on the seagrass, Cymodocea nodosa, in a controlled environment. Plant samples, water, and soil were taken from the Bay of Cádiz, Spain, and placed in aquaria in a temperature-controlled room. One control group was left …

On The Composistion Of Cymodocea Nodosa Root Exudate Under Artificial Blue, Green And Natural Light Conditions, Armand Martin, Ben Manriquez, Christian Pompa, Aaron Saper, Kyle A. Grice, Jason Bystriansky

DePaul Discoveries

Seagrasses are identified as a sentinel species: a good indicator of overall marine ecosystem health and function. At the rhizome, they are known to interact with marine bacteria by exchanging energy in the form of glucose and free amino acids secreted through root exudate in exchange for microbe-fixated nitrogen that can be utilized for plant growth. To analyze potential outcomes of possible future changes in light availability, an experiment was designed to collect and analyze the root exudate of Cymodocea nodosa under three light conditions (standard fluorescent light, blue LED, and green LED light). After 72 hours of treatment, the …

Comparing Carapa Guianensis Seed Production In 3 Amazonian Forests, Carmen Valencia

DePaul Discoveries

This paper is the first part of a project that has the purpose of creating models that help us better understand variation in seed production in Carapa guianensis, a species in the mahogany family. The goal of this paper is to visualize seed production patterns to inform species management. When this study is completed, it will aid local communities harvesting Carapa in projecting revenue from the oil produced from the seeds. Carapa is a masting species, which means it has an intermittent synchronous production of large seed crops. The major suspected causes of variation in seed production are resource …

A Playful Life Cycle Assessment Of The Environmental Impact Of Children's Toys, Madeline R. Robertson, Christie Klimas

DePaul Discoveries

Toys aid in children’s progression through developmental stages, yet toy production has an environmental impact. This study is the first comparative life cycle assessment of three children’s toys. A life cycle assessment quantifies the impact of an item in comparable impact categories (i.e. global warming potential in kg CO2 equivalents). In this study, we use open LCA to compare toy impact from production to use. The results indicate that the plastic polybutylene carried the highest impact in terms of global warming potential for our predominantly plastic toy. The addition of a battery to the plush dog increased the toy’s eutrophication …

A Life Cycle Assessment Of Jewelry, Jessica Fernandez, Christie Klimas

DePaul Discoveries

Jewelry is unique in that it is not a necessity, yet it holds sentimental and material value to the owner. Jewelry sales in the US have increased throughout time, meaning the demand for gold has almost always been increasing, as well. With more than half of the gold mined going towards the production of jewelry, it is the product with the highest demand of the resource. However, mining for the metals to produce a piece of jewelry, specifically gold, has negative consequences on both the environment and the people working in or living near mines. This study is a life …

Depaul Discoveries Volume 8 Cover, Jessica Hughes

DePaul Discoveries

No abstract provided.

Modification Of Host Behavior And Transmission In The Acanthocephalan Acanthocephalus Dirus: Effects Of Development, Intraspecific Conflict, And Host Sex, Sara R. Teemer

College of Science and Health Theses and Dissertations

Parasites are organisms that live on or in another in order to survive. In some cases, parasites require more than one host to complete their life cycle and rely on a predation event for transmission to the next host. Inside the host, the parasite must access host resources to grow and develop from the non-infective to infective stages. At the infective stage, the parasite is able to survive within the definitive host. Development to this stage has been correlated with changes in antipredatory behaviors, body size and color, and reproduction of intermediate hosts in ways that may increase predation by …

Body Forms In Sharks (Chondrichthyes: Elasmobranchii), And Their Functional, Ecological, And Evolutionary Implications, Phillip C. Sternes

College of Science and Health Theses and Dissertations

Sharks are among the oldest vertebrate lineages in which their success has been attributed to their diversity in body shape and locomotor design. In this study, I investigated the diversity of body forms in extant sharks using landmark-based geometric morphometric analyses on lateral view illustrations of nearly all the known (ca. 470) extant sharks in a published guidebook. I ran three different analyses: the ‘full body,' ‘precaudal body,’ and ‘caudal fin' analyses. My study suggests that there are two basic body forms in sharks. The two major body forms are characterized as a ‘shallow-bodied’ form (Group A) and ‘deep-bodied’ form …

Wavelet Analysis Of Short Globular Homologous Proteins In Mesophile And Thermophile Prokaryotes, John B. Linehan

College of Science and Health Theses and Dissertations

This study looked to identify features related to thermal stability and function in the amino acid chains of short globular proteins from mesophile and thermophile species, within the constraint that the protein fold to perform a speci_c function. To do so 540 homologous pairs of proteins were studied. The amino acid chains were con-verted to hydrophobicity signals by assigning a hydropathy score to each residue in the polypeptide. The hydrophobicity signals were passed through a wavelet packet transform and the resulting spectra analyzed. Bootstrapping was used to gener-ate a control data set to determine if the true ordering of amino …

Phylogeography Of The Neotropical Fish Genus Rhoadsia (Teleostei: Characidae) In Ecuador, Roberto Valentino Cucalón Tamayo

College of Science and Health Theses and Dissertations

Western Ecuador is considered a biodiversity hotspot. Nevertheless, studies of population genetic structure and variation are rare, especially in aquatic species. The genus Rhoadsia is an endemic freshwater fish in this region with two recognized species, Rhoadsia minor and R. altipinna. Little is known about the evolutionary relationships of their populations, and due to morphological similarities, their validity as distinct species has been questioned. The present study uses a phylogeographic approach to examine the evolutionary history of the genus and the validity of the two described species. Furthermore, I investigated the possible geographical origin of the genus based on patterns …

Sex Differences In Behavioral Responses To Repeat Subconcussive Events, Rebecca J. Wilson

College of Science and Health Theses and Dissertations

Although concussions, especially those in athletes and military, have become a popular focus of neurotrauma research, subconcussions occur with higher frequency and are less well-studied. A subconcussion is loosely defined as an impact to the head that does not result in a diagnosable concussion but can result in neuronal alterations. Repeat subconcussions have been shown to produce behavioral impairments along with neuropathology that is similar to or worse than those seen in a single concussion injury. These studies have primarily included male subjects. Given the potential effects of hormones and NIH’s call for sex-inclusion in biomedical research, assessing female responses …