Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 3 of 3
Full-Text Articles in Life Sciences
Study Of Role Of Ribosomal Protein L13a In Resolving Inflammation, Darshana Poddar
Study Of Role Of Ribosomal Protein L13a In Resolving Inflammation, Darshana Poddar
ETD Archive
Inflammation is an obligatory attempt of the host immune system to protect the body against infection. However, unregulated synthesis of pro-inflammatory products can have detrimental effects. Though mechanisms which contribute to inflammation are well appreciated, those that resolve inflammation are poorly understood. Therefore, understanding the molecular basis of such pathways will provide an entirely novel approach to treat and prevent inflammatory diseases. Transcript-selective translational control can regulate the expression of a set of inflammatory genes. We have identified one such mechanism in a novel animal model which relies on the abrogation of ribosomal protein L13a-dependent translational silencing by creating macrophage-specific …
Study Of The L13a Residues Required For Ribosomal Function, Priyanka Das
Study Of The L13a Residues Required For Ribosomal Function, Priyanka Das
ETD Archive
Ribosome biogenesis, a fundamental process, occurs in the nucleolus. It involves incorporation and association of ribosomal proteins (r-proteins) in the ribosomal subunit. Despite its obligatory and critical role in cellular function, the explicit mechanism of incorporation of different r-proteins into the pre-ribosome is not well understood. Using mammalian cell and r-protein L13a as our model, this study addresses the function and mechanism of r-protein incorporation during ribosome maturation. Published results from our laboratory showed the requirement of the release of L13a from the 60S ribosome to silence a cohort of inflammatory proteins directly at the level of translation thus showing …
An Insight Into Gait Complex Mediated Translational Silencing, Purvi Kapasi
An Insight Into Gait Complex Mediated Translational Silencing, Purvi Kapasi
ETD Archive
Transcript-specific translational control restricts macrophage inflammatory gene expression. The pero-inflammatory cytokine IFN-Îđ induces the phosphorylation of human ribosomal protein L13a and its subsequent release from 60S ribosome. L13a is a component of the interferon-gamma-activated inhibitor of translation (GAIT). The GAIT complex binds a defined element in the 3'-untranslated region (UTR) of ceruloplasmin (Cp) mRNA and causes delayed silencing of translation. In this research, we elucidate the molecular mechanism underlying L13a translational silencing activity. L13a mediates translational silencing particularly, when driven by internal ribosome entry sites (IRESs) that requires the initiation factor eIF4G, but is resistant to silencing when driven by …