Life Sciences Commons^™

The Effect Of Percussive Massage On Collagen Gene Expression In Skeletal Muscle, James Bartling, Robert D. Hyldahl, Emma Schaugaard, Mohadeseh Ahmadi

Library/Life Sciences Undergraduate Poster Competition 2024

INTRODUCTION

• Exercise is a critical aspect of healthy living due in part to its effects of muscle adaptation, which are largely regulated by alterations in gene expression

• Sedentary behavior can be required by diverse life circumstances, suggesting a need to reproduce the beneficial effects of exercise by an alternative means

• Like exercise, percussive massage introduces a mechanical strain on the muscle, which could potentially replicate a similar remodeling response of the extracellular matrix (ECM). However, this has not yet been studied

• Collagen 1,3,&4 are major components of the ECM that adapt in response to exercise, and …

Changes In Rna Expression Of Hut78 Cells Resulting From The Hiv-1 Viral Protein R R77q Mutation, Joshua S. Ramsey

Theses and Dissertations

Human immunodeficiency virus type 1 (HIV-1) is the causative virus for acquired immunodeficiency syndrome (AIDS). AIDS is characterized by chronic inflammation and reduction of CD4+ T-cells in the blood. This leads to the patient becoming immunocompromised and much more susceptible to disease in general. Different phenotypes in the progression of AIDS have been observed in patients in either progressing to AIDS faster as a Rapid Progressor (RP), or slower as a Long-Term Non-Progressor (LTNP). Researching elements that result in the LTNP phenotype is of interest as it has the potential to offer alternative treatments and therapies to those suffering from …

Adversarial Deep Neural Networks Effectively Remove Nonlinear Batch Effects From Gene-Expression Data, Jonathan Bryan Dayton

Theses and Dissertations

Gene-expression profiling enables researchers to quantify transcription levels in cells, thus providing insight into functional mechanisms of diseases and other biological processes. However, because of the high dimensionality of these data and the sensitivity of measuring equipment, expression data often contains unwanted confounding effects that can skew analysis. For example, collecting data in multiple runs causes nontrivial differences in the data (known as batch effects), known covariates that are not of interest to the study may have strong effects, and there may be large systemic effects when integrating multiple expression datasets. Additionally, many of these confounding effects represent higher-order interactions …

Histone Modifications And Their Effects On Nucleosome Positioning And Gene Expression, Jordon Ritchie, Steven Johnson

Journal of Undergraduate Research

Nucleosome positioning plays an important role in gene regulation and expression. Nucleosomes consist of DNA-histone interactions that comprise the first order of DNA compaction into chromatin in the cell. Modifications to the histone in the nucleosome have been hypothesized to influence the location of the nucleosome on the DNA and therefore the regulation of the gene the nucleosome is forming on. In our original proposal, we proposed to show the effects that different modifications had on the position of the nucleosome on the DNA and the DNA sequence that had the highest affinity for nucleosome formation given a specific histone …

Gene Expression Patterns In Flea Vectors Of Yersinia Pestis, Wei Zhou

Theses and Dissertations

Plague bacteria (Yersinia pestis) are transmitted to susceptible mammals by fleas. At least 25 flea species found in North America have been identified as plague vectors. The most efficient flea vector is the Oriental rat flea Xenopsylla cheopis, while the cat flea Ctenocephalides felis is a poor vector. The factors that determine vector competence of different fleas are not known. The main obstacles that the bacteria must overcome in the flea gut are also unknown. Fleas' molecular responses to Y. pestis invading could be a determining factor to control the bacterial survival and growth. Good and poor …

Duplicate Gene Expression In Allopolyploid Gossypium Reveals Two Temporally Distinct Phases Of Expression Evolution, Joshua Udall, Lex E. Flagel, Dan Nettleton, Jonathan F. Wendel

Faculty Publications

Polyploidy has played a prominent role in shaping the genomic architecture of the angiosperms. Through allopolyploidization, several modern Gossypium (cotton) species contain two divergent, although largely redundant genomes. Owing to this redundancy, these genomes can play host to an array of evolutionary processes that act on duplicate genes. Results- We compared homoeolog (genes duplicated by polyploidy) contributions to the transcriptome of a natural allopolyploid and a synthetic interspecific F1 hybrid, both derived from a merger between diploid species from the Gossypium A-genome and D-genome groups. Relative levels of A- and D-genome contributions to the petal transcriptome were determined for 1,383 …

Expression And Evolutionary Relationships Of The Chenopodium Quinoa 11s Seed Storage Protein Gene, Mikel Stevens, Peter J. Maughan, Daniel J. Fairbanks, Marie R. B. Balzotti, Jennifer N. Thornton, David A. Mcclellan, Eric N. Jellen, Craig E. Coleman

Faculty Publications

Quinoa (Chenopodium quinoa Willd.) is a food crop cultivated by subsistence farmers and commercial growers on the high Andean plateau, primarily in Bolivia, Peru, and Chile. Present interest in quinoa is due to its tolerance of harsh environments and its nutritional value. It is thought that the seed storage proteins of quinoa, particularly the 11S globulins and 2S albumins, are responsible for the relatively high protein content and ideal amino acid balance of the quinoa seed. Here we report the genomic and cDNA sequences for two 11S genes representing two orthologous loci from the quinoa genome. Important features of the …