Life Sciences Commons^™

Lake Mead National Recreation Area Monitoring And Evaluation Of Sensitive Wildlife: Quarterly Progress Report, Period Ending December 31, 2010, Margaret N. Rees

Wildlife Monitoring

Project 1. Relict Leopard Frog Monitoring, Management, and Research

• Final milestones and deliverables for this project were completed this quarter.
• Fall monitoring surveys were completed at all sites.
• Habitat actions to improve breeding pools were conducted at one site.
• Data and associated metadata were compiled and quality assured.
• Final and annual reports were written and submitted in required formats for Clark County

and the Relict Leopard Frog Conservation Team.

Project 2. Bald Eagle Winter Monitoring and Evaluation

• This project was previously completed and all associated deliverables met.
• Efforts were provided this quarter in support of the 2011 winter count.

Project …

Revegetating Burned Arid Lands: Identifying Successful Native Species Using Trait And Competition Analysis: Final Report, Scott R. Abella

Fire Science

Species selection can make the difference between successful revegetation projects and costly failures. Candidate native species for revegetating burned arid lands in the southwestern United States must meet at least two criteria. These species must: (1) be competitive in postfire environments typically dominated by exotic grasses, and (2) be able to become established reliably by seeding or planting. In response to Manager’s Request Task 3 (reestablishment of native vegetation after fires on arid lands) in the 2006 JFS announcement for proposals, this research tackled the problem of selecting native species with the greatest chance of revegetation success by conducting synergistic …

Enhancement Of Reactive Oxygen Species Production And Chlamydial Infection By The Mitochondrial Nod-Like Family Member Nlrx1, Ali A. Abdul-Sater, Najwene Saïd-Sadier, Verissa M. Lam, Bhavni Singh, Matthew A. Pettengill, Fraser Soares, Ivan Tattoli, Simone Lipinski, Stephen E. Girardin, Philip Rosenstiel, David M. Ojcius

All Dugoni School of Dentistry Faculty Articles

Chlamydia trachomatis infections cause severe and irreversible damage that can lead to infertility and blindness in both males and females. Following infection of epithelial cells, Chlamydia induces production of reactive oxygen species (ROS). Unconventionally, Chlamydiae use ROS to their advantage by activating caspase-1, which contributes to chlamydial growth. NLRX1, a member of the Nod-like receptor family that translocates to the mitochondria, can augment ROS production from the mitochondria following Shigella flexneri infections. However, in general, ROS can also be produced by membrane-bound NADPH oxidases. Given the importance of ROS-induced caspase-1 activation in growth of the chlamydial vacuole, we investigated the …

Optimization Of Application Of Delactosed Whey Permeate Treatment To Extend The Shelf-Life Of Fresh Cut Tomato Using Response Surface Methodology., Lubna Ahmed, Ana Belen Martin-Diana, Daniel Rico, Catherine Barry-Ryan

Articles

Optimization of delactosed whey permeate (DWP) treatment for fresh-cut tomato was accomplished by evaluating different quality, nutritional and microbial markers. Response surface methodology was applied to obtain polynomial model equations. DWP concentration (0 - 5 %) and storage (0 - 10 days) were used as independent factors in order to optimize the process. The analyses showed that increases in DWP concentration extended the quality of the fresh-cut tomato significantly (p3 % were scored unacceptable by the sensory panel due to perceived off-odours. DWP treatment also improved retention of ascorbic acid and lycopene over storage. The total aerobic counts and yeast …

Lamprey Watershed Education And Outreach, Lamprey River Watershed Association, Dawn Genes, Lamprey River Watershed Association. Lamprey River Nominating Committee

PREP Reports & Publications

The Lamprey River Nomination Committee sought the assistance of the Piscataqua Region Estuaries Partnership (PREP) to complete an extensive and detailed information package to nominate the Lamprey River and its major tributaries to the NH Rivers Management and Protection Program. The nomination package was due at DES on June 1, 2010, was reviewed and accepted by the State Rivers Management Committee in September and forwarded to the Commissioner of DES. The nomination is now working through the NH legislature with hearings anticipated during the spring 2011 session. If passed, the bill should be signed by Governor Lynch in July of …

Determination Of The Effect Of Dairy Powders On Adherence Of Streptococcus Sobrinus And Streptococcus Salivarius To Hydroxylapatite And Growth Of These Bacteria, Rachel Halpin, D.B. Brady, E.D. O’Riordan, M. O’Sullivan

Articles

Dental caries is a highly prevalent disease caused by colonisation of tooth surfaces by cariogenic bacteria, such as Streptococcus sobrinus and Streptococcus salivarius. Reducing initial adherence of such bacteria to teeth may delay onset of caries. Many foods, such as milk, can inhibit microbial adherence. In this investigation, the effect of untreated (UT) and enzyme-treated (ET) dairy powders on adherence of S. sobrinus and S. salivarius to hydroxylapatite (HA), an analogue of tooth enamel, was examined. Untreated (UT) acid whey protein concentrate (AWPC) 80 inhibited streptococcal adherence to phosphate-buffered saline-coated HA (PBS-HA) and saliva-coated HA (S-HA) by >80% at …

Due Diligence And Demographic Disparities: Effects Of The Planning Of U.S.- Mexico Border Fence On Marginalized Populations, J. Gaines Wilson, Jude A. Benavides, Karen Engle, Denise Gilman, Anthony Reisinger, Jessica Spangler, Joe Lemen

School of Earth, Environmental, and Marine Sciences Faculty Publications and Presentations

In the buildup to the construction the U.S.-Mexico border fence by the United States Department of Homeland Security (USDHS), much attention was drawn to security, drug enforcement, and immigration issues. However, there was little quantitative analysis regarding which populations were most likely to be affected by the proposed fence. Using a geographic information system, we classified census blocks in Cameron County, Texas into one of two categories: either ‘fence’ or ‘gap’. A total of 14 demographic factors were tested for disparities between those living in gap areas and those living in areas exposed to the fence. Twelve of fourteen factors …

Global Patterns And Predictions Of Seafloor Biomass Using Random Forests, Chih-Lin Wei, Gilbert T. Rowe, Elva Escobar-Briones, Antje Boetius, Thomas Soltwedel, M. Julian Caley, Yousria Soliman, Falk Huettmann, Fangyuan Qu, Zishan Yu, C. Roland Pitcher, Richard L. Haedrich, Mary K. Wicksten, Michael A. Rex, Jeffrey G. Baguley, Jyotsna Sharma, Roberto Danovaro, Ian R. Macdonald, Clifton C. Nunnally, Jody W. Deming, Paul Montagna, Mélanie Lévesque, Jan Marcin Weslawski, Maria Wlodarska-Kowalczuk, Baban S. Ingole, Brian J. Bett, David S. M. Billett, Andrew Yool, Bodil A. Bluhm, Katrin Iken, Bhavani E. Narayanaswamy

Biology Faculty Publication Series

A comprehensive seafloor biomass and abundance database has been constructed from 24 oceanographic institutions worldwide within the Census of Marine Life (CoML) field projects. The machine-learning algorithm, Random Forests, was employed to model and predict seafloor standing stocks from surface primary production, water-column integrated and export particulate organic matter (POM), seafloor relief, and bottom water properties. The predictive models explain 63% to 88% of stock variance among the major size groups. Individual and composite maps of predicted global seafloor biomass and abundance are generated for bacteria, meiofauna, macrofauna, and megafauna (invertebrates and fishes). Patterns of benthic standing stocks were positive …

Conjugated Polymer Nanoparticles For Effective Sirna Delivery To Tobacco By-2 Protoplasts, Asitha T. Silva, Alien Nguyen, Changming Ye, Jeanmarie Verchot, Joong Ho Moon

Department of Chemistry and Biochemistry

Background

Post transcriptional gene silencing (PTGS) is a mechanism harnessed by plant biologists to knock down gene expression. siRNAs contribute to PTGS that are synthesized from mRNAs or viral RNAs and function to guide cellular endoribonucleases to target mRNAs for degradation. Plant biologists have employed electroporation to deliver artificial siRNAs to plant protoplasts to study gene expression mechanisms at the single cell level. One drawback of electroporation is the extensive loss of viable protoplasts that occurs as a result of the transfection technology.

Results

We employed fluorescent conjugated polymer nanoparticles (CPNs) to deliver siRNAs and knockdown a target gene in …

A Study To Minimize Or Eliminate Hardbottom And Reef Impacts From Anchoring Activities In Designated Anchorages At The Ports Of Miami And Palm Beach, Brian K. Walker

Marine & Environmental Sciences Faculty Reports

No abstract provided.

Spatial Distribution Of Calcium-Gated Chloride Channels In Olfactory Cilia, Donald A. French, Dorjsuren Badamdorj, Steven J. Kleene

Mathematical Sciences Faculty Research

Background

In vertebrate olfactory receptor neurons, sensory cilia transduce odor stimuli into changes in neuronal membrane potential. The voltage changes are primarily caused by the sequential openings of two types of channel: a cyclic-nucleotide-gated (CNG) cationic channel and a calcium-gated chloride channel. In frog, the cilia are 25 to 200 µm in length, so the spatial distributions of the channels may be an important determinant of odor sensitivity.

Principal Findings

To determine the spatial distribution of the chloride channels, we recorded from single cilia as calcium was allowed to diffuse down the length of the cilium and activate the channels. …

Conclusions About Niche Expansion In Introduced Impatiens Walleriana Populations Depend On Method Of Analysis, Lisa Mandle, Dan L. Warren, Matthias H. Hoffman, A. Townsend Peterson, Johanna Schmitt, Eric J. Von Wettberg

Department of Biological Sciences

Determining the degree to which climate niches are conserved across plant species’ native and introduced ranges is valuable to developing successful strategies to limit the introduction and spread of invasive plants, and also has important ecological and evolutionary implications. Here, we test whether climate niches differ between native and introduced populations of Impatiens walleriana, globally one of the most popular horticultural species. We use approaches based on both raw climate data associated with occurrence points and ecological niche models (ENMs) developed with Maxent. We include comparisons of climate niche breadth in both geographic and environmental spaces, taking into account differences …

Oregon Aspen Project, Forest Restoration Partnership

Aspen Bibliography

The Oregon Aspen Project was initiated in response to the decline of aspen groves in Oregon and throughout the Western United States, and the lack of information to guide managers interested in stewardship to enhance this resource. There were three central goals and accompanying objectives for this project which are listed below. The project accomplishments are provided below each bulleted objective

Strategies To Resolve The Three-Dimensional Structure Of The Genome Of Small Single-Stranded Icosahedral Viruses, Eduardo Sanz Garcia

Theses and Dissertations

The aim of this study is the three-dimensional structural characterization of the genome packaging inside viral capsids via cryo-electron microscopy and three-dimensional reconstruction. The genome of some single-stranded viruses can be densely packaged within their capsid shells. Several stretches of the genome are known to adopt stable secondary structures, however, to date, little is known about the three-dimensional organization of the genome inside their capsid shells. Two techniques have been developed to facilitate the structural elucidation of genome packaging: the asymmetric random-model method, and the symmetry-mismatch, random model method. Both techniques were successfully tested with model and experimental data. The …

Biphasic Targeting And Cleavage Furrow Ingression Directed By The Tail Of A Myosin Ii, X. Fang, J. Y. Luo, R. Nishihama, C. Wloka, C. Dravis, M. Travaglia, M. Iwase, Elizabeth Ann Vallen, E. Bi

Biology Faculty Works

Cytokinesis in animal and fungal cells utilizes a contractile actomyosin ring (AMR). However, how myosin II is targeted to the division site and promotes AMR assembly, and how the AMR coordinates with membrane trafficking during cytokinesis, remains poorly understood. Here we show that Myo1 is a two-headed myosin II in Saccharomyces cerevisiae, and that Myo1 localizes to the division site via two distinct targeting signals in its tail that act sequentially during the cell cycle. Before cytokinesis, Myo1 localization depends on the septin-binding protein Bni5. During cytokinesis, Myo1 localization depends on the IQGAP Iqg1. We also show that the Myo1 …

Pengaruh Tambahan Asupan Kalium Dari Diet Terhadap Penurunan Hipertensi Sistolik Tingkat Sedang Pada Lanjut Usia, Yuli Amran, Febrianti Febrianti, Lies Irawanti

Kesmas

Hipertensi adalah salah satu penyebab kematian utama di dunia dengan mortalitas akibat hipertensi sistolik 2,5 kali lipat lebih besar daripada mortalitas akibat hipertensi diastolik. Hipertensi yang tidak diobati menyebabkan komplikasi pada beberapa target organ seperti jantung, mata, ginjal, dan otak, sementara pengobatan hipertensi termasuk pengendalian diet harus dilakukan secara berkesinambungan sepanjang hidup. Guna menurunkan kasus-kasus hipertensi, suatu penelitian kuasi eksperimen dengan rancangan rangkaian waktu telah dilakukan di Panti Werdha Islamic Village Tangerang, Provinsi Banten, untuk mengamati pengaruh tambahan asupan kalium dalam diet terhadap penurunan tekanan darah sistolik. Buah-buahan yang lebih banyak ditambahkan ke dalam diet harian untuk 12 orang lanjut …

Northeast Research Station Watertown, South Dakota Annual Progress Report, 2010, Agricultural Experiment Station, Plant Science Department

Agricultural Experiment Station and Research Farm Annual Reports

This is the 2010 annual progress report for the Northeast Research Station in Watertown, South Dakota. This report is issued by the South Dakota Agricultural Experiment Station and the South Dakota State University Plant Science Department. This report includes weather data, yield comparisons, crop performance trials, corn trials, soybean trials, winter wheat performance testing, Barley Foliar information, weed and pest control, herbicide demonstrations, canola and flax variety trials and more.

Complexity Through Recombination: From Chemistry To Biology, Niles Lehman, Carolina Diaz Arenas, Wesley A. White, Francis J. Schmidt

Chemistry Faculty Publications and Presentations

Recombination is a common event in nature, with examples in physics, chemistry, and biology. This process is characterized by the spontaneous reorganization of structural units to form new entities. Upon reorganization, the complexity of the overall system can change. In particular the components of the system can now experience a new response to externally applied selection criteria, such that the evolutionary trajectory of the system is altered. In this work we explore the link between chemical and biological forms of recombination. We estimate how the net system complexity changes, through analysis of RNA-RNA recombination and by mathematical modeling. Our results …

Reduction Of Iron (Iii) And Humic Substances Plays A Major Role In Anaerobic Respiration In An Arctic Peat Soil, David A. Lipson, Mony Jha, Ted K. Raab, Walter C. Oechel

Ted K. Raab

Arctic peat soils contain vast reserves of organic C and are largely anaerobic. However, anaerobic respiration, particularly the role of Fe(III) and humic substances as electron acceptors, is not well understood in such ecosystems. We investigated these processes in a drained thaw lake basin on the Arctic coastal plain near Barrow, Alaska. We measured concentrations of soluble Fe and other potential electron acceptors, described the microbial community, and performed experiments in the laboratory and field to measure net rates of Fe(III) reduction and the relationship of this process to C cycling. In most areas within the basin, aerobic conditions existed …

Smokejumper Obituary: Steiner, Robert Carl (Boise 1971), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.

Role For Sumoylation In Systemic Inflammation And Immune Homeostasis In Drosophila Larvae, Indira Paddibhatla, Mark J. Lee, Marta E. Kalamarz, Roberto Ferrarese, Shubha Govind

Publications and Research

To counter systemic risk of infection by parasitic wasps, Drosophila larvae activate humoral immunity in the fat body and mount a robust cellular response resulting in encapsulation of the wasp egg. Innate immune reactions are tightly regulated and are resolved within hours. To understand the mechanisms underlying activation and resolution of the egg encapsulation response and examine if failure of the latter develops into systemic inflammatory disease, we correlated parasitic wasp-induced changes in the Drosophila larva with systemic chronic conditions in sumoylation-deficient mutants. We have previously reported that loss of either Cactus, the Drosophila (IkB) protein or Ubc9, the SUMO-conjugating …

Symposium Support: Integrative Biology Of Animal Regeneration - Seattle, Wa January 2010, Sara M. Lindsay, Alexandra Bely

University of Maine Office of Research Administration: Grant Reports

Certain animals can regrow (regenerate) lost body parts, whereas others cannot. This is a fascinating and important area of biology, but why only some animals have this ability is still poorly understood. The purpose of this conference symposium is to bring together researchers working on regeneration from a variety of research perspectives in order to foster integrative approaches to studying this question. Ten researchers working from different perspectives in regeneration biology, ranging from molecular and cell biology to ecology, will present current research findings and participants will discuss the state of the field and needed areas for future research. Additional …

Minimum Description Length Measures Of Evidence For Enrichment, Zhenyu Yang, David R. Bickel

COBRA Preprint Series

In order to functionally interpret differentially expressed genes or other discovered features, researchers seek to detect enrichment in the form of overrepresentation of discovered features associated with a biological process. Most enrichment methods treat the p-value as the measure of evidence using a statistical test such as the binomial test, Fisher's exact test or the hypergeometric test. However, the p-value is not interpretable as a measure of evidence apart from adjustments in light of the sample size. As a measure of evidence supporting one hypothesis over the other, the Bayes factor (BF) overcomes this drawback of the p-value but lacks …

Growth Inhibition Of Common Food Spoilage And Pathogenic Microorganisms In The Presence Of Brown Seaweed Extracts, Shilpi Gupta, Sabrina Cox, Gaurav Rajauria, Amit Jaiswal, Nissreen Abu-Ghannam

Articles

The possibility of using extracts from brown seaweed, Himanthalia elongata, as a natural antimicrobial agent for food preservation is presented. The effect of different concentrations of seaweed extract on the growth kinetics of four common food spoilage (Pseudomonas aeruginosa and Enterococcus faecalis) and food pathogenic micro-organisms (Listeria monocytogenes and Salmonella abony) was examined. Seaweed extract at a concentration of 6% inhibited the growth of all four of the studied organisms. Lower concentrations of seaweed extract prolonged the lag phase and reduced both the exponential growth rate and final population densities of the culture. Suitability of three kinetic models, Baranyi-Roberts, Modified …

Development Of Endothermy In Nestling Bank Swallows (Riparia Riparia), Richard L. Marsh

Richard Marsh

Body temperature (Tb) measurements after exposure to air temperatures (Tₐ) of 20 C or 27 C for 2 h in conjunction with metabolism measurements were used to describe the timing of and basis for developing temperature regulation in nestling swallows. As growth proceeds from hatching to 10 g there is a gradual increase in the Tb after exposure to low Tₐ. As growth continues beyond this point, the ability of nestlings to maintain Tb above Tₐ improves rapidly, such that nestlings weighing over 14 g are completely homeothermic when exposed to 20 C. Conductance (C, in cal [g⋅h⋅°C]⁻¹) decreases throughout …

Effects On Nestling Age And Burrow Depth On Co₂ And O₂ Concentrations In The Burrows Of Bank Swallows (Riparia Riparia), Steven J. Wickler, Richard L. Marsh

Richard Marsh

Gas samples were taken from the nest chambers of bank swallows (Riparia riparia) and analyzed for CO₂ and O₂ content. The mean CO₂ content was 2.62% with a maximum value of 5.58%, and the mean O₂ content was 17.83% with a minimum value of 14.61%. There was a positive and significant correlation of increasing CO₂ content with both increasing nestling age and increasing total metabolizing mass (adults plus young). With increasing metabolizing mass there is a linear increase in CO₂ content, which suggests no active regulation of gas concentrations. Burrow depth also affected CO₂, particularly in burrows with older nestlings. …

Winter Fattening In The American Goldfinch And The Possible Role Of Temperature In Its Regulation, William R. Dawson, Richard L. Marsh

Richard Marsh

We investigated whether environmental temperature has any causal role in the winter fattening in certain finches of the subfamily Carduelinae. Correlational analyses between fat content of American goldfinches (Carduelis tristis) and various short- and long-term measures of temperature provide no evidence for a proximate role of this environmental variable in determining the degree of fattening of these birds in southeastern Michigan. Their fat content shows the best correlations (r = −.61 to −.63) with the long-term average minimum temperature or record low temperature for the date of capture. Furthermore, inclusion of long-term thermal measures in multivariate analyses excludes from significance …

Adaptations Of The Gray Catbird Dumetella Carolinensis To Long-Distance Migration: Flight Muscle Hypertrophy Associated With Elevated Body Mass, Richard L. Marsh

Richard Marsh

The size and composition (lean-dry, water, and fat contents) of the flight muscles of the catbird were investigated as a function of the large seasonal changes in body mass which occur in this species. The mass of the pectoralis muscle is highly positively correlated with body mass, leading to an elevation in muscle mass of ∼35% during fall premigratory fattening. The changes in muscle mass are brought about by coordinated variations in all major components of the muscles which were measured. High-oxidative, fast-twitch fibers represent 88% of the total fibers in the pectoralis muscle. The cross-sectional area of the muscle …

The Enzymatic Basis Of High Metabolic Rates In Calling Frogs, Theodore L. Taigen, Kentwood D. Wells, Richard L. Marsh

Richard Marsh

Oxygen consumption by male spring peepers (Hyla crucifer) increased linearly with calling rate, reaching peak values of 1.5−1.7 ml O₂/(g⋅h) at the highest calling rates. The intercept of the regression line describing the relationship between metabolism and calling rate does not differ significantly from daytime resting metabolism (0.11 ml O₂/[g⋅h]). Metabolic rate during vigorous locomotor exercise at the same temperature (19 C) was only 1.1 ml O₂/(g⋅h). We measured activities of mitochondrial enzymes in the trunk muscles (internal and external obliques) involved in sound production and in mixed hind limb muscles of male and female frogs. Male trunk muscles were …

Measurement Of Maximum Oxygen Consumption In Guinea Fowl Numida Meleagris Indicates That Birds And Mammals Display A Similar Diversity Of Aerobic Scopes During Running, David J. Ellerby, Maryellen Cleary, Richard L. Marsh, Cindy I. Buchanan

Richard Marsh

Judgement of exercise performance in birds has been hampered by a paucity of data on maximal aerobic capacity. We measured the maximal rate of oxygen consumption (V̇o₂,max) in running guinea fowl Numida meleagris, a bird that has been used in several previous studies of avian running. Mean V̇o₂,max during level treadmill running was 97.5±3.7 mL O&8322; kg⁻¹ min⁻¹ (mean ± SEM, N=5). V̇o₂,max was on average 6% higher when the birds ran uphill compared with the value during level running (paired t-test, P=0.041, N=5). The mean basal rate of oxygen consumption (V̇o₂,bmr) of the same individuals was 7.9±0.5 mL kg⁻¹ …