Life Sciences Commons^™

Microvascular Architecture Of The Filiform Papillae In Primates And Insectivores, S. Okada, Y. Ohta, N. Matsukawa, S. Sugioka

Scanning Microscopy

The microvascular architecture of filiform papillae was investigated under a scanning electron microscope in man, Japanese monkeys, common squirrel monkeys, common marmosets, common tree shrews, large Japanese moles and dwarf shrews utilizing microvascular corrosion casts. Filiform papillae were circularly arranged in primates, and each of them was supplied by a hairpin capillary loop. These papillae sometimes were aggregated. The filiform papillae of Japanese monkeys exhibited markedly locational differences on the lingual dorsum and were supplied by circularly arranged capillary loops or by an intrapapillary capillary network. Small filiform papillae were located on an epithelial eminence in the lingual radix, each …

Modelling Techniques For The Quantification Of Some Electron Beam Induced Phenomena, Wk Chim, Dsh Chan, Ts Low, Jch Phang, Ks Sim, Kl Pey

Scanning Microscopy

This paper presents simulation models for quantifying the voltage contrast, cathodoluminescence and indirect specimen charging phenomena in the scanning electron microscope (SEM). The voltage contrast model comprises an electric field computation program using the finite-element approach, and a secondary electron trajectory tracking algorithm employing a linear electric field assumption. This trajectory tracking algorithm is more accurate than the conventional electron trajectory tracking algorithms which make use of a constant electric field assumption within each computation step. Using this model, results of qualitative voltage contrast effects on secondary electron trajectories in the specimen chamber of the SEM are shown. This model …

Scanning Electron Microscopic Studies Of The Oral Mucosa And Its Microvasculature: A Review Of The Palatine Mucosa And Its Microvascular Architecture In Mammals, Y. Ohta, S. Okada, I. Toda, H. Ike

Scanning Microscopy

The present paper deals with the microvascular architecture of the palatine mucosa in primates, camivorae, and rodentia utilizing microvascular corrosion castings and epithelium-separated specimens.

The submucous vascular network is under-developed since the bard palatine mucosa was designated the mucoperiosteum, except some areas. The palatine venous plexus appears to show regional differences with animal species differences. The well-developed plexus is observed to be two-layered and may contribute to the process of regurgitation of rough food and assist in mastication with the palatine plicae. Formation and patterns of the arterial network in the lamina propria are in a close relation with connective …

An Improved Method For Preparing Microvascular Corrosion Casts Of Rat Embryos, S. Yoshida, J. Chiba

Scanning Microscopy

This paper presents an improved method for preparing microvascular corrosion casts of rat embryos (day 16.5-21.5). Special attention was paid on the viscosity of the casting material, the method of mechanical restraint, and the subsequent drying of the casts. The embryos were perfused with Mercox resin diluted with 25% methyl methacrylate monomer followed by undiluted Mercox resin to avoid outflow of resin from the vasculature after perfusion. The specimens were mounted on a stainless steel plate with self-curing resin to prevent flotation, mechanical damage, and collapse of the cast specimens following preparation. After digestion and washing, the cast specimens were …

Microstructural Differences Among Adzuki Bean (Vigna Angularis) Cultivars, Anup Engquist, Barry G. Swanson

Food Structure

Scanning electron microscopy (SEM) was used to study microstructural differences among five adzuki bean cultivars: Erimo, Express, Hatsune, Takara and VBSC. Seed coat surfaces showed different patterns of cracks, pits and deposits . Cross-sections of the seed coats revealed well organized layers of elongated palisade cells followed by many layers of amorphous parenchyma cells. Typical sub-epidermal layers of organized columnar, hour-glass cells were characteristica11y absent in the five cultivars of adzuki beans. The cross-sections of the cotyledons revealed intercellular materials between storage cells. Storage cells in Hatsune cultivar were loosely packed with many intercellular spaces as compared to the other …

Image Segmentation Applied To Scanning Electron Microscopy Multi-Images Of Weathered Stones Of Monuments, M. Rautureau, R. Harba, G. Jacquet

Scanning Microscopy

This paper describes a three complementary images processing method. The three images are coming from a scanning electron microscope (SEM) during the analysis of a particular stone: the Tuffeau used in most monuments of the Loire valley (France). The goal is to separate two classes of particles (calcareous and siliceous) from the porosity to give more information to experts who evaluate the damage of weathering on monuments. A specific process is developed: a first threshold on the good quality image allows separation of particles from porosity. Then, the complementarity of the three images gives the two other thresholds. Granulometry, percentages …

Scanning Electron Microscopy Structure And Firmness Of Papain Treated Apple Slices, Yaguang Luo, Max E. Patterson, Barry G. Swanson

Food Structure

'Mcintosh' apple (Malus domesrica Borkh.) slices were treated with papain. Textural changes were recorded with an Instron Universal Testing Machine. Structural changes and distribution of microorganisms in apple tissues after treatment were observed with a scanning electron microscope (SEM). Apple slices submerg ed in a 1% papain solution were significantly firmer than apple slices submerged in the distilled water control for a 24 hour period (P < 0.05). Three and four days after slicing , a significantly smaller decay index was observed on the apple slices submerged in papain solution than on the control slices. Under SEM, less severe cell wall breakdown was observed on the apple tissues treated with papain than on apple tissues without treatment. Less spores were also observed on the papain treated apple slices than apple slices without treatment. Apple tissues treated with papain solution and distilled water also demonstrated noticeable st ru ctural differences. The apple tissues treated with papain solution for 18 hours retained the original cell structure while the cells in the apple tissues treated with distilled water collapsed.