Life Sciences Commons^™

The Relationship Between The Murine Primary Immune Response To Sheep Red Blood Cells & Heterosis, Russell Gailor

Masters Theses & Specialist Projects

Two inbred strains of mice, BALB/c and C57BL, were crossbred to produce Fl hybrids. This breeding scheme ensured a high degree of genetic heterozygosity as exemplified by the fact that the average weight of the Fl hybrids at 10 weeks of age was 10.1 percent greater that that of their contemporaries in the inbred parental strains.

The three lines of mice were then challenged with a 0.5 ml intraperitoneal injection of two-percent sheep red blood cells (SRBC). After a 15-day period the mice were bled and their sera were microtitrated for anti-SRBC agglutinin and the titers statistically analyzed. The Fl …

A Comparison Of Chemical Composition & Fermentation Patterns Of Alternative Silages To Whole Plant Corn Silage, Susan Fox

Masters Theses & Specialist Projects

A comparison of the relationship of fermentation to chemical composition was made for forages which were wilted and ensiled at 35 to 45 percent dry matter. Trial I consisted of three forages ensiled in October, 1981: interseeded soybeans and grain sorghum. whole plant corn with added anhydrous ammonia, and whole plant corn with shelled corn added at a rate of 150 kg/t of fresh forage. Trial II consisted of two forages ensiled in 1982: interseeded soybeans and pearlmillet, and wheat. Temperatures of fermentation were collected, and chemical composition during the first 25 days of fermentation analyzed.

Production data were also …

Studies On I) Dry Matter And Nitrogen Disappearance Of Six Soybean Protein Products In Situ And Ii) Contamination Of In Situ Dry Matter And Nitrogen Disappearance With Acid Detergent Fiber, James Coomer

Masters Theses & Specialist Projects

In experiment I), dry matter disappearance (DMD) and nitrogen disappearance (ND) of raw soybeans (RAW), solvent extracted soybean meal (SBM), heat treated whole soybeans (HT), mechanically extracted soybean meal (MEX), dry extruded soybeans (DEX), and wet extruded soybeans (TEX), were studied in situ for times of 3, 6, 12 and 24 h of rumen exposure. Five gram, air dry, samples were suspended in the rumen of a lactating Holstein cow fed a total mixed ration twice daily. The percent DMD for 24 h was as follows: RAW-85.9; SBM-56.6; HT-39.0; MEX-40.2; DEX-28.0; TEX-43.3. The greatest DMD was observed with RAW and …

Partial Purification & Characterization Of A Carboxylesterase From The Greater Wax Moth Larva, David Garst

Masters Theses & Specialist Projects

Various properties of a carboxylesterase of the greater wax moth larva, Galleria melionella (L.), and a commercial carboxylic ester hydrolase (EC 3.1.1.1) from porcine liver were examined. The larval enzyme was partially purified. Esterase activities were determined with acetylsalicylic acid, 0-acetyl-m-hydroxybenzoic acid, and 0-acetyl-phydroxybenzoic acid in tris buffer (0.05 M tris, 0.1 M EDTA, pH 7.85). The larval enzyme was partially purified by a combination of acetone powder preparation, ammonium sulfate precipitation, anion exchange chromatography, 0-200 gel filtration, and polyacrylamide gel electrophoresis (PAGE).

The acetone powder preparation was initially obtained and tested for esterolytic activity. The ammonium sulfate fractionation greatly …

The Effects Of Spacings & Cultivars On Biological Yields, Yields, Component Of Yield & Harvest Index Of Green Bush Snap Bean (Phaseolus Vulgaris L.), Widodo

Masters Theses & Specialist Projects

The effects of cultivars and plant spacings on biological yield, seed yield, seeds per pod, average seed weight, and harvest index of beans (Phaseolus vulgaris L.) were studied in 1987 and 1988. Two cultivars, 'Blue Lake 27' and 'Kentucky Wonder 125' and four plant spacings -- 7, 14, 21, and 28 cm, constituted eight treatments that were arranged in a split-plot with four replications. Each experimental unit or hill consisted of five plants -- four in the corners and one in the center of a square. Spacings treatment were established by varying the distance between the center plant …

Effect Of Phosphorus Placement Methods On The Yield & Quality Of Tomatoes, Timothy Hambrick

Masters Theses & Specialist Projects

Research was undertaken in the summer and fall of 1988 to determine the effect of phosphorus placed below, below and to the side, banded and incorporated, and broadcast on the yield and quality of tomatoes.

The results of the field study were not statistically significant due to high levels of available soil phosphorus. However, there were trends toward higher total yields, higher yield of number one grade tomatoes, and higher tissue phosphorus levels when phosphorus was placed in a concentrated zone or band as contrasted with the more popular broadcast method.

The effect of phosphorus placement in the greenhouse was …

Isolation & Characterization Of A Type I Topoisomerase From The Hypocotyls Of Etiolated Soybeans, Rick Dye

Masters Theses & Specialist Projects

Topoisomerases are enzymes of critical biological significance. Despite this fact, little data specific to the topoisomerases of higher plants has been published. This research was undertaken to isolate and characterize a higher plant topoisomerase from soybean hypocotyls to further the understanding of the structure and function of these enzymes.

Nuclei were isolated from frozen hypocotyls of 4 day old etiolated soybeans by homogenization and centrifugation, then lysed by gentle stirring in the presence of 1.5 mM ammonium sulfate. The resultant extract was desalted and purified by column chromatography on DEAF sepharose, 5-200 sephacryl and CM cellulose. The components of the …