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Articles 1 - 5 of 5
Full-Text Articles in Life Sciences
Reproductive Timing In Percina Aurora And Other Percina Species In The Pascagoula River Watershed, Malia Davidson
Reproductive Timing In Percina Aurora And Other Percina Species In The Pascagoula River Watershed, Malia Davidson
Honors Theses
The Southeastern United States is home to high levels of aquatic biodiversity and was recently named one of the Earth’s biodiversity hotspots. Within this region, growing human populations continue to place pressure on aquatic ecosystems and the resident species. Darters (Percidae: Percina) represent a large proportion of the freshwater fish diversity in the region. However, for some species the fundamentals of age, growth, reproductive timing and effort, and ecology remain poorly understood. That said, understanding the timing, duration, and effort allocated to reproduction is vital to successful management of any species. The pearl darter, Percina aurora, has been …
Multi-Generational Effects Of ∆9-Tetrahydrocannabinol Exposure On Gene Expression In Liver Tissue, Kayla Lovitt
Multi-Generational Effects Of ∆9-Tetrahydrocannabinol Exposure On Gene Expression In Liver Tissue, Kayla Lovitt
Honors Theses
Cannabis is the most commonly used, cultivated, and trafficked illicit drug worldwide. Increased availability and acceptance of cannabis and cannabinoid-containing products provide the necessity for understanding how these substances influence aging. In this study, zebrafish (Danio rerio) were exposed to concentrations of Δ9-tetrahydrocannabinol (THC) (0.08, 0.4, 2 µM) during embryonic-larval development, the effects on aging were measured 30 months later and in the offspring of the exposed fish (F1 generation. We observed results indicating a biphasic and hormetic effect. Treatment with the lowest concentration of THC significantly increased egg production, while higher concentrations resulted in impaired …
Mitochondrial Dna Inheritance In Bivalves: A Comparative Study Involving The Unique System Of Doubly Uniparental Inheritance, Sarah Heishman
Mitochondrial Dna Inheritance In Bivalves: A Comparative Study Involving The Unique System Of Doubly Uniparental Inheritance, Sarah Heishman
Honors Theses
A comparative study of Doubly Uniparental Inheritance (DUI) in the bivalve mollusks Mytilus edulis and Geukensia demissa has yielded unanticipated results. Around the world, it has been reported that members of the taxonomic family Mytilidae (along with the families of Unionidae and Veneridae) consistently exhibit DUI. However, the hard-to-place Geukensia demissa, which is a member of this family, has had varying reports of its DUI status. Most reports involving G. demissa vary with the location in which it is being studied, which prompts more questions than it answers. Due to many months of unsuccessful DNA purification attempts, sequencing and an …
Ubiquitin-Mediated Proteolysis During Gametogenis In Chlamydomonas Reinhardtii, Hall Thomas Mcgee
Ubiquitin-Mediated Proteolysis During Gametogenis In Chlamydomonas Reinhardtii, Hall Thomas Mcgee
Honors Theses
Scientists in the field of cell biology have focused considerable attention on a unicellular species of green algae known as Chlamydomonas reinhardtii. This organism is relatively common in bodies of fresh water and some types of soil, but most of the attention is due to its value as a model for biochemical studies. This laboratory has undertaken a broad study of the species including exploration into a special class of proteins, called heat shock proteins, and certain biochemical processes of the organism. The work herein described is a part of this latter study, and also a preliminary step in a …
Sds Non-Acrylamide Polymeric Gel-Filled Capillary Electrophoresis For Molecular Size Separation Of Protein, Devon Andres
Sds Non-Acrylamide Polymeric Gel-Filled Capillary Electrophoresis For Molecular Size Separation Of Protein, Devon Andres
Honors Theses
Sodium dodecyl sulfide (SDS) non-acrylamide gel-filled capillary columns are a new technology being used for analysis and separation of biotechnology-derived proteins. This research was to compare this new technology to the current methods of SDS polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance size-exclusion chromatography (HPSEC). The molecular mass of four different recombinant proteins were determined by two commercialized SDS non-acrylamide gel-filled capillary columns, SDS-PAGE, and HPSEC. The data obtained showed that the SDS non-acrylamide gel-filled capillary columns were compatible with the SDS-PAGE technique for molecular mass determination. HPSEC was shown to be unreliable for molecular weight determination. SDS non-acrylamide gel-filled capillary …