Life Sciences Commons^™

Nutritional Status And Growth In Infants With Cystic Fibrosis At Diagnosis And At Age Two Years And Six Years, Marianne Rich

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

PURPOSE: A retrospective chart review was conducted to determine if nutritional source of feeding and/or infant age at diagnosis of cystic fibrosis had any effect on nutritional status and subsequent growth accretion. Additionally, an attempt was made to identify predictors for poor growth in patients with undiagnosed cystic fibrosis.

METHODS: Data was collected from medical and clinic charts at Primary Children's Medical Center (PCMC), Salt Lake City, Utah, for subjects born between January 1, 1995 and December 31, 200 I, who were diagnosed with cystic fibrosis before 1 year of age. Thirty-one subjects met inclusion parameters. These subjects were divided …

Identification Of Factors Affecting Susceptibility To Depression And Influencing Quality Of Life In Adult Cystic Fibrosis Patients Based On Age-At-Diagnosis, Marlene Israelsen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Approximately 10% of cystic fibrosis (CF) cases remain undiagnosed until age 18 or later and medical professionals are still unclear about the needs of these individuals. Adults with CF must either adapt to life with a chronic disease or transition into adulthood with CF. The purpose of this study was to address the impact of age-at-diagnosis on quality of life in a CF adult population.

The psychological functioning of 21 adult CF patients (seven adult-diagnosed (AD) and 14 pediatric-diagnosed (PD)) was assessed using the Beck Depression Index (BDI)-II. A descriptive review of demographic and clinical data, which included an assessment …

Nutrition Education For Parents Of Children With Cystic Fibrosis : Assessment Of Needs And Strategies For Improved Knowledge And Confidence In Self Care, Catherine M. Mcdonald

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Suboptimal growth and nutritional status are problematic for children with cystic fibrosis (CF). Optimal nutrition predicts better lung function and longevity. Daily nutrition therapy for children with CF requires adequate food resources, knowledge of appropriate nutrition and behavior management skills, and confidence in one's ability to correctly apply the necessary skills. The Mountain West Cystic Fibrosis Consortium Questionnaire (MWCFC-Q) was designed as an educational needs assessment for parents of children with CF. The goal was to identify areas of concern that could be targeted for educational intervention to ultimately improve children's growth and nutritional status.

Data analyzed from 305 returned …

Nutrition And Bone Density In Children With Cystic Fibrosis, Joanna K. Davidson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of these studies was to further research on bone density in children with cystic fibrosis, particularly as it pertains to nutritional parameters and care. The first paper presented a comparison of a group of 50 children with cystic fibrosis to a group of 32 control children. There were no significant differences between the groups in any of the pertinent bone density measurements. Serum 25(OH) vitamin D was positively correlated with spine density z score in the cystic fibrosis group.

The second paper, incorporating all of the information obtained from the first paper, describes an intervention study with the …

X-Ray Microanalysis Of Epithelial And Secretory Cells In Culture, Anne Von Euler, Eva Pålsgård, Christina Vult Von Steyern, Godfried M. Roomans

Scanning Microscopy

Cell cultures can be used to study ion transport processes. X-ray microanalysis of cell cultures at the cellular level gives interesting information that can complement electrophysiological and tracer studies. In this paper, methods for culturing and preparing a variety of epithelial and secretory cells (fibroblasts, insulinoma cells, bovine mammary epithelial cells, colon cancer cells) for X-ray microanalysis are presented. Results show that sometimes cell cultures are not homogeneous with respect to ion content or reaction to physiological stimuli. In colon cancer cell cultures, a "high K" and a "low K" cell subpopulation was found; these subpopulations also differed with respect …

Effect Of Chronic Treatment With Diuretics On Mouse Liver: A Morphological And Microanalytical Investigation, Ann-Christin Mork, Anne Von Euler, Godfried M. Roomans

Scanning Microscopy

In an attempt to produce an animal model for the disease cystic fibrosis (CF), mice were treated chronically with the diuretics amiloride and furosemide, in order to cause chronic inhibition of transepithelial ion transport. Experiments were carried out on adult mice (2 months treatment); in addition, pregnant mice were treated with diuretics, and tissue from offspring 2 and 7 days post partum was investigated. Since biliary cirrhosis is a common occurrence in CF, hepatocytes in the treated mice were investigated by X-ray microanalysis and by light and electron microscopy.

Treatment with amiloride caused a significant decrease in cellular Na concentration …

Changes In Rat And Mouse Salivary Glands And Pancreas After Chronic Treatment With Diuretics: A Potential Animal Model For Cystic Fibrosis, Samuel Sagström, Edward B. Mcmillan, Monique Marijianowski, Helmy Mulders, Godfried M. Roomans

Scanning Microscopy

Defective transepithelial chloride and water transport is thought to be the cellular basis of the disease cystic fibrosis (CF). Therefore, it was attempted to develop an animal model for this disease by chronically inhibiting transepithelial chloride transport in experimental animals by long term treatment with high doses of diuretics. In the present study, changes in the salivary glands and pancreas after such treatment were investigated by X-ray microanalysis and electron microscopy.

Treatment of rats for one month with diuretics caused a significant decrease in chloride and an increase in calcium in the acinar cells of the submandibular gland. This increase …

Strontium And Bromide As Tracers In X-Ray Microanalysis Of Biological Tissue, Joanna Wroblewski, Samuel Sagström, Helmy Mulders, Godfried M. Roomans

Scanning Microscopy

Since energy dispersive X-ray microanalysis cannot distinguish between isotopes of the same element, alternative methods have to be used to get information similar to that obtained in experiments with radioactive tracers. In the present study, strontium was used as a tracer for calcium, and bromide as a tracer for chloride. Rats were injected with strontium chloride in vivo, and the uptake of strontium in the acinar cells of the submandibular gland was studied. Eventually a Sr/Ca ratio of 0.3 was reached. In some animals, secretion of mucus had been elicited by stimulation with isoproterenol 4 h prior to injection …

Element Concentrations In The Intestinal Mucosa Of The Mouse As Measured By X-Ray Microanalysis, Thomas Von Zglinicki, Godfried M. Roomans

Scanning Microscopy

Subcellular ion distribution in villus, crypt, Paneth and smooth muscle cells of the mouse small intestine under resting conditions was investigated by X-ray microanalysis of ultrathin cryosections. In addition, the mass distribution was estimated by measuring the optical transmission of the compartments in transmission electron micrographs.

Each cell type is characterized by a special composition in terms of the major monovalent ions Na, K, and Cl. In particular, among crypt epithelial cells, those cells containing small secretion granula (termed crypt A cells) also display cytoplasmic ion concentrations significantly different from crypt epithelial cells lacking secretion granula (crypt B cells). Monovalent …

Effect Of Duct Obstruction On Structure, Elemental Composition, And Function Of Rat Submandibular Glands, Samuel Sagström, Gun-Britt Sagulin, Godfried M. Roomans

Scanning Microscopy

Obstruction of salivary glands occurs in association with a number of pathological conditions. It has been suggested that the major changes found in the salivary glands of patients with cystic fibrosis are due to obstruction of the excretory duct by viscous mucus. In the present study, the effect of excretory duct obstruction on structure, elemental composition and function of rat submandibular gland was investigated. Obstruction was effected by infusion of a fast-hardening protein emulsion in the main excretory duct. After 1 week, and more pronounced after 2 weeks of obstruction the number of granular duct cells had decreased in the …

X-Ray Microanalysis Of Mammalian Salivary Glands, Godfried M. Roomans, R. Margareta Müller, Samuel Sagström, Gun-Britt Sagulin, Susan M. Scarlett, Joanna Wroblewski, Maria Albertsson

Scanning Microscopy

X-ray microanalysis was used to study the elemental composition of major salivary glands in rat and man. The elemental composition of the acinar cells is influenced by a variety of factors. Stimulation of fluid secretion by cholinergic, α-adrenergic or peptidergic agonists leads to a sustained increase of the Na/K ratio. The elemental composition and ultrastructure of the acinar cells of rat submandibular gland, as well as flow rate and composition of pilocarpinestimulated submandibular saliva are significantly affected by thyroxine, but less by the synthetic glucocorticoid dexamethasone. Hypercalcemia (induced by chronic vitamin D treatment) as well as hypocalcemia (induced by calcitonin) …

Surface Morphology Of Human Airway Mucosa: Normal, Carcinoma Or Cystic Fibrosis, P. K. Jeffery, A. P. R. Brain

Scanning Microscopy

The study presents preliminary qualitative findings of an investigation of grossly normal main and lobar bronchi at sites distant to well circumscribed tumour (n=15), adjacent to tumour (n=5) or of airways obtained during heart/lung transplantation in patients with cystic fibrosis (CF, n=3). In the normal airways the surface epithelium was on average 50 𝝁m thick, pseudostratified and rested on a roughly contoured basement membrane. A variety of cell types were identified although many were obscured by a dense covering of cilia, occasionally interrupted by foci of squamous metaplasia. Submucosal gland structure was observed in chance vertical fractures of the airway …

Calcium And Cystic Fibrosis, Godfried M. Roomans

Scanning Electron Microscopy

Cystic fibrosis (CF) is a generally lethal, congenital, genetic disease of unknown etiology. It is likely that a defective regulation of ion and water transport in exocrine glands and possibly also in other epithelial cells has a central role in the pathogenesis of this disease. Calcium has been implicated in the basic defect underlying CF because of findings of abnormally high calcium levels in some secreted fluids and some cells of CF patients.

Using X-ray microanalysis, we have demonstrated elevated calcium concentrations in cultured fibroblasts and in goblet cells of the bronchial epithelium of CF patients. A factor produced by …

Post-Mortem Storage Of Tissue For X-Ray Microanalysis In Pathology, Godfried M. Roomans, Joanna Wroblewski

Scanning Electron Microscopy

Possible alternatives to rapid freezing in liquid nitrogen of tissue for X- ray microanalysis of electrolytes at the cellular level were investigated. These alternatives might be used in cases where tissue becomes available for examination, e.g., at autopsy, but liquid nitrogen is not immediately available. Rat submandibular gland was used as a test tissue. Freezing of pieces of tissue in a conventional freezer at -80°C or even at -20°C retained the elemental distribution at the cellular level, and also retained the difference between a 'normal' and a 'pathological' (mimicked by an inject ion of a high dose of isoproterenol) situation. …