Life Sciences Commons^™

Effectiveness Of Dentin Bonding Agents, J. -F. Roulet, U. Blunck

Scanning Microscopy

The interface between a composite resin restoration and dentin is still a problem with regard to secondary caries and esthetics. With conventional restorative techniques the absence of bonding between the restorative material and the dentin produces marginal gaps. These gaps are populated with microorganisms or trap pigments. Therefore, adhesive techniques are required for functional and esthetic restorations.

In extracted teeth stored in 0.1% thymol solution, 8 cylindrical cavities (diameter: 3 mm; depth: 2 mm) and 8 class V cavities (1/2 of the margin in dentin) were prepared in each group and filled with composite resin. Before actual placement the following …

The Effects Of Retinoic Acid And Butyric Acid On In Vitro Migration By Murine B16a Cells: A Quantitative Scanning Electron Microscopic Study, T. W. Mcgarvey, B. Persky

Scanning Microscopy

Retinoic acid (RA) and butyric acid (BA) were investigated for their effect on in vitro migration of highly metastatic murine B16a melanoma cells. These potential antitumor agents are known to alter the cytoskeleton. Our initial studies determined the 72 h cytostatic /cytotoxic concentration of RA (1 X 10^-6 M / > 1 X 10^-5M) and BA (1.5 mM) / > 2.0 mM). Cytostasis by RA and BA was confirmed by autoradiography and radioisotope incorporation. For migration assays, cells were plated on 3 and 5 μm diameter pore polycarbonate membranes. Complete media was added containing RA or BA at time …

In Vitro Identification Of The Effect Of Serotonin On Lymphocyte Dna Synthesis And Natural Killer Cell Activity, Kim Kartchener Kane

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of this study was to identify the effects of the neurotransmitter, serotonin (SE), on the immune function of peripheral blood mononuclear cells (PBMC) of normal, healthy subjects. This was done as a preliminary investigation to studies on the association of SE with immune changes in autistic subjects.

The PBMC isolated from normal male subjects were treated with various concentrations of SE for 48 hrs. Their incubation in SE at a concentration of 10^-3 M induced about a 35% decrease in DNA synthesis. However, incubation of the cells in lower concentrations (10^-4 to 10^-10) of …