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Articles 1 - 5 of 5
Full-Text Articles in Life Sciences
Identification Of Plasmalogens In The Cytoplasmic Membrane Of Bifidobacterium Animalis Subsp. Lactis, T. S. Oberg, Robert E. Ward, J. L. Steele, Jeff Broadbent
Identification Of Plasmalogens In The Cytoplasmic Membrane Of Bifidobacterium Animalis Subsp. Lactis, T. S. Oberg, Robert E. Ward, J. L. Steele, Jeff Broadbent
Nutrition, Dietetics, and Food Sciences Faculty Publications
Plasmalogens are ether-linked lipids that may influence oxidative stress resistance of eukaryotic cell membranes. Since bacterial membrane composition can influence environmental stress resistance, we explored the prevalence of plasmalogens in the cytoplasmic membrane of Bifidobacterium animalis subsp. lactis. Results showed plasmalogens are a major component of the B. animalis subsp. lactis membrane.
Analysis Of The Lactobacilluscasei Supragenome And Its Influence In Species Evolution And Lifestyle Adaptation, Jeff Broadbent, E. C. Neeno-Eckwall, B. Stahl, K. Tandee, H. Cai, W. Morovic, P. Horvath, J. Heidenreich, N. T. Perna, R. Barrangou, J. L. Steele
Analysis Of The Lactobacilluscasei Supragenome And Its Influence In Species Evolution And Lifestyle Adaptation, Jeff Broadbent, E. C. Neeno-Eckwall, B. Stahl, K. Tandee, H. Cai, W. Morovic, P. Horvath, J. Heidenreich, N. T. Perna, R. Barrangou, J. L. Steele
Nutrition, Dietetics, and Food Sciences Faculty Publications
The broad ecological distribution of L. casei makes it an insightful subject for research on genome evolution and lifestyle adaptation. To explore evolutionary mechanisms that determine genomic diversity of L. casei, we performed comparative analysis of 17 L. casei genomes representing strains collected from dairy, plant, and human sources. Results Differences in L. casei genome inventory revealed an open pan-genome comprised of 1,715 core and 4,220 accessory genes. Extrapolation of pan-genome data indicates L. casei has a supragenome approximately 3.2 times larger than the average genome of individual strains. Evidence suggests horizontal gene transfer from other bacterial species, particularly lactobacilli, …
A Complete Genome Sequence Oflactobacillus Helveticus R0052, A Commercial Probiotic Strain, T. A. Tompkins, G. Barreau, Jeff Broadbent
A Complete Genome Sequence Oflactobacillus Helveticus R0052, A Commercial Probiotic Strain, T. A. Tompkins, G. Barreau, Jeff Broadbent
Nutrition, Dietetics, and Food Sciences Faculty Publications
Lactobacillus helveticus R0052 is a commercially available strain that is widely used in probiotic preparations. The genome sequence consisted of 2,129,425 bases. Comparative analysis showed that it was unique among L. helveticus strains in that it contained genes encoding mucus-binding proteins similar to those found in Lactobacillus acidophilus.
Survival Of Microencapsulated Probiotic Lactobacillus Paracasei Lbc-1e During Manufacture Of Mozzarellacheese And Simulated Gastric Digestion, Megan Jane Ostler, Jeff Broadbent, W. R. Mcmanus, D. J. Mcmahon
Survival Of Microencapsulated Probiotic Lactobacillus Paracasei Lbc-1e During Manufacture Of Mozzarellacheese And Simulated Gastric Digestion, Megan Jane Ostler, Jeff Broadbent, W. R. Mcmanus, D. J. Mcmahon
Nutrition, Dietetics, and Food Sciences Faculty Publications
An erythromycin-resistant strain of probiotic Lactobacillus paracasei ssp. paracasei LBC-1 (LBC-1e) was added to part-skim Mozzarella cheese in alginate-microencapsulated or free form at a level of 108 and 107 cfu/g, respectively. Survival of LBC-1e and total lactic acid bacteria (LAB) was investigated through the pasta filata process of cheese making (in which the cheese curd was heated to 55°C and stretched in 70°C-hot brine), followed by storage at 4°C for 6 wk and simulated gastric and intestinal digestion. This included incubation in 0.1 M and 0.01 M HCl, 0.9 M H3PO4, and a simulated intestinal juice consisting of pancreatin and …
Optimal Growth Oflactobacillus Casei In A Cheddar Cheese Ripening Model System Requires Exogenous Fatty Acids, W. S. Tan, M. F. Budinich, Robert E. Ward, Jeff Broadbent, J. L. Steele
Optimal Growth Oflactobacillus Casei In A Cheddar Cheese Ripening Model System Requires Exogenous Fatty Acids, W. S. Tan, M. F. Budinich, Robert E. Ward, Jeff Broadbent, J. L. Steele
Nutrition, Dietetics, and Food Sciences Faculty Publications
Flavor development in ripening Cheddar cheese depends on complex microbial and biochemical processes that are difficult to study in natural cheese. Thus, our group has developed Cheddar cheese extract (CCE) as a model system to study these processes. In previous work, we found that CCE supported growth of Lactobacillus casei, one of the most prominent nonstarter lactic acid bacteria (NSLAB) species found in ripening Cheddar cheese, to a final cell density of 108 cfu/mL at 37°C. However, when similar growth experiments were performed at 8°C in CCE derived from 4-mo-old cheese (4mCCE), the final cell densities obtained were only about …