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Use Of Trpe/Gag Fusion Proteins To Characterize Immunoreactive Domains On The Human Immunodeficiency Virus Type 1 Core Protein, Michael Windheuser, Gary Tegtmeier, Charles Wood
Use Of Trpe/Gag Fusion Proteins To Characterize Immunoreactive Domains On The Human Immunodeficiency Virus Type 1 Core Protein, Michael Windheuser, Gary Tegtmeier, Charles Wood
Nebraska Center for Virology: Faculty Publications
The human immunodeficiency virus (HIV) p24 core protein is one of the most immunogenic of HIV structural proteins. Infected individuals develop high titers of antibodies against p24 early in infection, which makes anti-p24 antibodies important serological markers. However, despite the clinical importance of the anti-p24 response, no systematic study to characterize the antigenic domains on the p24 protein has been reported. We report here on the use of 12 overlapping fragments of the HIV type 1 p24 protein, synthesized in bacteria as TrpE/Gag fusion proteins, to identify at least two and possibly three antigenic domains on the p24 protein. In …
Effect Of Milk On Fibronectin And Collagen Type I Binding To Staphylococcus Aureus And Coagulase-Negative Staphylococci Isolated From Bovine Mastitis, J. Miedzobrodzki, A. S. Naidu, J. L. Watts, Pawel Ciborowski, K. Palm
Effect Of Milk On Fibronectin And Collagen Type I Binding To Staphylococcus Aureus And Coagulase-Negative Staphylococci Isolated From Bovine Mastitis, J. Miedzobrodzki, A. S. Naidu, J. L. Watts, Pawel Ciborowski, K. Palm
Nebraska Center for Virology: Faculty Publications
Tryptic soy broth (TSB)-grown cells of Staphylococcus aureus isolated from acute and chronic bovine mastitis bound mainly 125I-fibronectin (125I-Fn), whereas strains of nine species of coagulase-negative staphylococci showed a predominant interaction with 125I-collagen (125I-Cn) type I. A particle agglutination assay (PAA) was used to examine the interaction of coagulase-negative staphylococci with 1251-Fn and 125I-Cn immobilized on latex. All 368 coagulase-negative staphylococci demonstrated high 125I-Cn and moderate to low 125I-Fn interactions in the PAA. Cn-PAA reactivity was high among strains of Staphylococcus xylosus (84.2%), Staphylococcus simulans (77.8%), Staphylococcus epidermidis (76.7%), and …
Transactivation Of Human Immunodeficiency Virus Promoter By Human Herpesvirus 6, Rebecca Horvat, Charles Wood, N. Balachandran
Transactivation Of Human Immunodeficiency Virus Promoter By Human Herpesvirus 6, Rebecca Horvat, Charles Wood, N. Balachandran
Nebraska Center for Virology: Faculty Publications
Patients with acquired immunodeficiency syndrome (AIDS) are often infected with a number of other heterologous viruses in addition to the initial human immunodeficiency virus (HIV) infection, and these agents could act as potential reactivating agents of latent HIV. A new antigenically distinct herpesvirus, designated human herpesvirus 6 (HHV-6), has recently been isolated from patients with AIDS and has been shown to infect a number of different human cells, specifically human T cells, B cells, and glial cells. Since these are some of the same cells that harbor the AIDS virus, it is quite important to determine any interaction between this …
Use Of Trpe/Gag Fusion Proteins To Characterize Immunoreactive Domains On The Human Immunodeficiency Virus Type 1 Core Protein, Michael Windheuser, Gary Tegtmeier, Charles Wood
Use Of Trpe/Gag Fusion Proteins To Characterize Immunoreactive Domains On The Human Immunodeficiency Virus Type 1 Core Protein, Michael Windheuser, Gary Tegtmeier, Charles Wood
Nebraska Center for Virology: Faculty Publications
The human immunodeficiency virus (HIV) p24 core protein is one of the most immunogenic of HIV structural proteins. Infected individuals develop high titers of antibodies against p24 early in infection, which makes anti-p24 antibodies important serological markers. However, despite the clinical importance of the anti-p24 response, no systematic study to characterize the antigenic domains on the p24 protein has been reported. We report here on the use of 12 overlapping fragments of the HIV type 1 p24 protein, synthesized in bacteria as TrpE/Gag fusion proteins, to identify at least two and possibly three antigenic domains on the p24 protein. In …