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Globular Domain Structure And Function Of Restriction-Like-Endonuclease Bearing Long Interspersed Nucleotide Elements, Mst Murshida Mahbub

Biology Dissertations

Long Interspersed Nucleotide Elements (LINEs) are a major group of eukaryotic transposable elements that have profoundly influenced and sculpted eukaryotic genome structure and function. LINEs replicate within the host genome, often to high copy number. Replication occurs through an RNA intermediate, which is integrated back into the host genome by target primed reverse transcription (TPRT). The element encoded protein is known to contain a restriction-like DNA endonuclease, a reverse transcriptase, and nucleic acid binding domains. However, the secondary and tertiary structure of these domains as well as the overall protein is poorly understood. The protein encoded by the R2 element …