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Action Of Repeat-Induced Point Mutation On Both Strands Of A Duplex And On Tandem Duplications Of Various Sizes In Neurospora, Michael Watters, Thomas Randall, Brian Margolin, Eric Selker, David Stadler

Michael Watters

In Neurospora crassa, DNA sequence duplications are detected and altered efficiently during the sexual cycle by a process known as RIP (repeat-induced point mutation). Affected sequences are subjected to multiple GC-to-AT mutations. To explore the pattern in which base changes are laid down by RIP we examined two sets of strains. First, we examined the products of a presumptive spontaneous RIP event at the mtr locus. Results of sequencing suggested that a single RIP event produces two distinct patterns of change, descended from the two strands of an affected DNA duplex. Equivalent results were obtained using an exceptional tetrad from …