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Cj1121c, A Novel Udp-4-Keto-6-Deoxy-Glcnac C-4 Aminotransferase Essential For Protein Glycosylation And Virulence In Campylobacter Jejuni, Somalinga Vijayakumar, Alexandra Merkx-Jacques, Dinath Ratnayake, Irene Gryski, Ravinder Obhi, Sébastien Houle, Charles M Dozois, Carole Creuzenet
Cj1121c, A Novel Udp-4-Keto-6-Deoxy-Glcnac C-4 Aminotransferase Essential For Protein Glycosylation And Virulence In Campylobacter Jejuni, Somalinga Vijayakumar, Alexandra Merkx-Jacques, Dinath Ratnayake, Irene Gryski, Ravinder Obhi, Sébastien Houle, Charles M Dozois, Carole Creuzenet
Alexandra Merkx-Jacques
Campylobacter jejuni produces glycoproteins that are essential for virulence. These glycoproteins carry diacetamidobacillosamine (DAB), a sugar that is not found in humans. Hence, the enzymes responsible for DAB synthesis represent potential therapeutic targets. We describe the biochemical characterization of Cj1121c, a putative aminotransferase encoded by the general protein glycosylation locus, to assess its role in DAB biosynthesis. By using overexpressed and affinity-purified enzyme, we demonstrate that Cj1121c has pyridoxal phosphate- and glutamate-dependent UDP-4-keto-6-deoxy-GlcNAc C-4 transaminase activity and produces UDP-4-amino-4,6-dideoxy-GlcNAc. This is consistent with a role in DAB biosynthesis and distinguishes Cj1121c from Cj1294, a homologous UDP-2-acetamido-2,6-dideoxy-beta-l-arabino-4-hexulose C-4 aminotransferase that we …