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Bactericidal Efficacy Of Liposomal Aminoglycosides Against Burkholderia Cenocepacia, Ali Azghani
Bactericidal Efficacy Of Liposomal Aminoglycosides Against Burkholderia Cenocepacia, Ali Azghani
Ali Azghani
Objectives Burkholderia cenocepacia (formally a genotype of Burkholderia cepacia complex called genomovar III) has emerged as a serious opportunistic pathogen in individuals with cystic fibrosis. We developed a liposomal antibiotic formulation composed of 1,2-distearoyl-sn-glycero-3-phosphocholine and cholesterol (molar ratio 2:1) to overcome B. cenocepacia's resistance to commonly used aminoglycosidic antibiotics.
Methods The dehydration-rehydration vesicles technique was used to entrap antibiotics in liposomes. The size of liposome formulations was measured and encapsulation efficiencies were determined by microbiological assays. MICs of free and liposomal antibiotics against the clinical isolates of B. cenocepacia were determined by the standard broth …