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From Pixels To Plants: Remote Sensing Of California Invasive Plants, Kenneth Rangel May 2024

From Pixels To Plants: Remote Sensing Of California Invasive Plants, Kenneth Rangel

Master's Projects and Capstones

Invasive plants cause significant impacts to ecosystems, the economy, and human health. California has experienced significant plant invasions and is well suited to future invasion because of its Mediterranean climate and human disturbance. Eradication or control of invasive plant species requires a detailed understanding of their spatial distribution, which typically involves on the ground surveys that can be expensive or inconsistent. Remote sensing offers a potential alternative or supplement to in-person invasive plant mapping. This study performed a comparative analysis of 41 remote sensing studies that mapped the distribution of California invasive plants. I found that while high spectral resolution …


Salinity Tolerance Of Flowering Rush, Butomus Umbellatus, Andrew Coomes May 2024

Salinity Tolerance Of Flowering Rush, Butomus Umbellatus, Andrew Coomes

Master's Theses

Flowering rush is an invasive aquatic plant in North America that causes deleterious effects to native ecosystems. There are two cytotypes, a triploid and diploid, and multiple genotypes contained within the diploid cytotype currently established in the U.S. Despite its presence in river drainages connected to estuarine areas, documentation on the salinity tolerance of flowering rush is scarce. Currently, information is limited to anecdotal reports suggesting intolerance to saline conditions. A better understanding of the osmotic tolerance of this species is essential to providing insight into its’ invasive range and better inform management efforts. These studies investigated vegetative propagule germination …


Impact Of Early-Season Postemergence Co-Applications Of Foliar And Residual Herbicides On Crop Injury, Growth, And Yield In 2,4-D- And Dicamba-Tolerant Cotton (Gossypium Hirsutum L.), Logan N. Vallee May 2024

Impact Of Early-Season Postemergence Co-Applications Of Foliar And Residual Herbicides On Crop Injury, Growth, And Yield In 2,4-D- And Dicamba-Tolerant Cotton (Gossypium Hirsutum L.), Logan N. Vallee

LSU Master's Theses

Studies were conducted in 2021 through 2023 at the LSU AgCenter Dean Lee Research and Extension Center near Alexandria, LA to determine the sensitivity of 2,4-D- or dicamba- tolerant cotton (Gossypium hirsutum L.) to an early season application of a Group 15 and foliar-only herbicides. Both studies were randomized complete block designs with a two-factorial arrangement of treatments with four replications. In the dicamba-tolerant cotton study, Factor A consisted of no foliar-herbicide, dicamba alone, glyphosate alone, or dicamba plus glyphosate. Factor B consisted of either no Group 15 residual herbicide, acetochlor, or S-metolachlor. In the 2,4-D- tolerant cotton …


Halosulfuron Plus Prosulfuron And Propanil Interactions For Weed Control In Louisiana Rice Production, John A. Williams Mar 2024

Halosulfuron Plus Prosulfuron And Propanil Interactions For Weed Control In Louisiana Rice Production, John A. Williams

LSU Master's Theses

A greenhouse study was conducted on November 18, and December 2, 2020 at Louisiana State University in Baton Rouge, Louisiana to evaluate the interaction between a pre-packaged mixture of halosulfuron plus prosulfuron mixed with propanil for alligatorweed control. Antagonism occurred when halosulfuron plus prosulfuron at 55 g ha-1 was mixed with propanil at 2,242 g ha-1 at all evaluation dates and antagonism was also observed with halosulfuron plus prosulfuron at 83 g ha-1 mixed with propanil at 2,242 g ha-1 at all evaluation dates except 14 DAT with observed controls of 69 to 77% compared with expected controls of 94 …


Ms Environmental Biology Capstone Project, Sarah Luper Jan 2024

Ms Environmental Biology Capstone Project, Sarah Luper

Regis University Student Publications (comprehensive collection)

Recreational trails and the presence of invasive species such as Bromus inermis can both disturb grassland community composition. Trails can act as dispersal corridors for plant material, but can also be a source of trampling and compaction. Invasive grasses like Bromus inermis can easily establish in grasslands and decrease species richness by increasing competition. Understanding these effects, this study aims to understand how both the presence of recreational trails as well as the presence of Bromus inermis affect species richness and overall community composition at Two Ponds National Wildlife Refuge. Belt transect sampling took place in fall 2019 and again …


Quantifying The Ecological Effects Of Salix Fragilis On Riparian Habitat In Kittitas County, Washington, Landon Shaffer Jan 2024

Quantifying The Ecological Effects Of Salix Fragilis On Riparian Habitat In Kittitas County, Washington, Landon Shaffer

All Master's Theses

Invasive species threaten plant community structure and function globally. Riparian areas, the zone near streams where water influences vegetation, are especially sensitive to invasive species colonization, suffering large-scale shifts in community composition. Salix fragilis (crack willow) is a nonnative riparian species abundant in the lower elevation tributaries of central Washington. Some speculate whether this willow should be listed as invasive in Washington, despite a lack of regional supporting evidence. I studied riparian communities dominated by either S. fragilis or native species in the Kittitas Valley and measured biodiversity, quantified differences in solar attenuation, and compared leaf decomposition rates to learn …


Exploring The Impact Of Invasive On Grassland Diversity: A Study In Montana, Ella Gaffney Jan 2024

Exploring The Impact Of Invasive On Grassland Diversity: A Study In Montana, Ella Gaffney

Undergraduate Theses, Professional Papers, and Capstone Artifacts

For the capstone project with the University of Montana Davidson Honors College, I investigated the diversity index of grassland and rangeland plots across the state of Montana. My study, conducted from June to August 2023, includes data collected by my team. The purpose of this research project was testing the hypothesis that the diversity index decreases in plots containing invasive grass species.

During the summer of 2023, the Spatial Analysis Lab surveyed over 1000 plots in rangelands and grasslands across the state of Montana to locate an invasive grass, Ventenata dubia. Data collected includes species identification, ocular estimates of …


Addressing Water Hyacinth (Pontederia Crassipes) Impacts On Aquatic Biota In Lake Okeechobee, Joseph Salerno Dec 2023

Addressing Water Hyacinth (Pontederia Crassipes) Impacts On Aquatic Biota In Lake Okeechobee, Joseph Salerno

All HCAS Student Capstones, Theses, and Dissertations

The incursion of water hyacinth, Pontederia crassipes in Lake Okeechobee has resulted in management systems to be implemented to reduce the coverage of the invasive macrophyte. Its residence in the Lake Okeechobee ecosystem and the effects it has on organisms in the lake, whether it be positive or harmful is unknown. This study attempted to assess the potential effects that water hyacinth has on aquatic biota in Lake Okeechobee. Biotic data were collected on open water, water hyacinth covered, and native vegetation covered habitats via hook-and-line fishing, electrofishing, baited minnow traps, and the sampling of plant roots over a thirteen-month …


Effect Of Cultural Strategies And Application Technologies On Weed Control In Flooded And Furrow-Irrigated Rice, Noah Hunter Reed Dec 2023

Effect Of Cultural Strategies And Application Technologies On Weed Control In Flooded And Furrow-Irrigated Rice, Noah Hunter Reed

Graduate Theses and Dissertations

Weed control is a major problem that rice (Oryza sativa L.) producers face in the Mid-south every growing season. Restricted use of some rice herbicides has also highlighted the need for more research to be conducted on cultural weed control strategies and application technologies in rice. The aim of these studies was to evaluate rice weed control using various rice row widths, bed widths (irrigation-furrow spacing), rice cultivar selection, and nozzle type selection. Three field experiments were conducted in 2021 and 2022 across four sites at Lonoke, AR, Pine Tree, AR, Rohwer, AR, and Stoneville, MS. In experiment one, as …


Evaluation Of Weed Control Efficacy And Crop Tolerance To Florpyrauxifen-Benzyl Coated Onto Urea, Bodie Lyn Cotter Dec 2023

Evaluation Of Weed Control Efficacy And Crop Tolerance To Florpyrauxifen-Benzyl Coated Onto Urea, Bodie Lyn Cotter

Graduate Theses and Dissertations

Frequent off-target movement occurrences to soybean [Glycine max (L.) Merr.], rice (Oryza sativa L.) injury, and a lack of barnyardgrass [Echinochloa crus-galli (L.) P. Beauv.] control followed the commercial launch of florpyrauxifen-benzyl in 2018. Florpyrauxifen-benzyl is a synthetic auxin, Herbicide Resistance Action Committee (HRAC)/Weed Science Society of America (WSSA) Group 4 herbicide used for postemergence weed control in rice and labeled for application at 30 g ae ha-1. Previous research documented that soybean was susceptible to injury following spray applications of florpyrauxifen-benzyl at less-than-labeled rates, and certain varieties of rice are less tolerant to spray-applied florpyrauxifen-benzyl at 30 g ae …


Furthering Indaziflam-Based Management Strategies Of Three Invasive Annual Grasses In Northern Utah, Peter Weston Maughan Dec 2023

Furthering Indaziflam-Based Management Strategies Of Three Invasive Annual Grasses In Northern Utah, Peter Weston Maughan

All Graduate Theses and Dissertations, Fall 2023 to Present

Indaziflam is an herbicide highly recognized for its potential to effectively control weedy, winter germinating grasses, such as downy brome, as the herbicides prevents seed germination for several years. Unfortunately, most of these studies only capture the first 2-3 years of indaziflam’s 3-5 years soil activity. This thesis sought to better understand indaziflam’s long-term effects by monitoring both a healthy ecosystem and a degraded ecosystem for up to 5 years after indaziflam application. A secondary study was also conducted to better understand the potential of revegetation via reseeding in indaziflam treated soils. The study concluded that indaziflam very little long-term …


Establishing And Optimization Of Tissue Culture Work With Erigeron Canadensis, Robert Petursson Dec 2023

Establishing And Optimization Of Tissue Culture Work With Erigeron Canadensis, Robert Petursson

Masters Theses

Glyphosate resistance among weed species has been an increasing problem in crop production over the past two decades. Glyphosate resistant Erigeron canadensis (horseweed) has been found on multiple continents and 20 U.S. states. It appears that the resistance is of non-target type, with mechanisms of action that have yet to be elucidated at the gene level. For the first portion of the study, I focused on reproducing the original indirect organogenesis protocol but discovered biotype variability can influence regeneration success. An improved indirect organogenesis method was then developed using the previously published methods that could be used across biotypes. A …


Containerized Satsuma Mandarin Production Under Protective Screens As A Management Strategy, Anna Timmerman Nov 2023

Containerized Satsuma Mandarin Production Under Protective Screens As A Management Strategy, Anna Timmerman

LSU Master's Theses

Many of the world’s citrus growing regions are experiencing challenges related to citrus greening, also known as Huanglongbing (HLB) caused by the presumed bacterial agent Candidatus Liberibacter spp. HLB is vectored by the Asian citrus psyllid (Diaphorina citri) and through propagation of infected citrus material. HLB impacts tree health as well as fruit development, ripening, and quality of the resulting citrus fruit, often leading to an unmarketable product. Producers are growing citrus in high density orchards underneath protective screening material that excludes the Asian citrus psyllid, preventing new HLB infections. Four ultra-high containerized tree spacings of 2 feet …


Biology And Herbicide Resistance Characteristics Of Various Annual Bluegrass (Poa Annua L.) Biotypes From Across The United States, Jacob William Taylor Aug 2023

Biology And Herbicide Resistance Characteristics Of Various Annual Bluegrass (Poa Annua L.) Biotypes From Across The United States, Jacob William Taylor

All Dissertations

Annual bluegrass (Poa annua L.) is one of the most problematic winter annual weeds in managed turfgrass systems, having rapidly adapted to different climates and management practices. Characteristics such as prolific seedhead production, clumped bunch-type growth habit, and lack of stress tolerance negatively impact turfgrass quality. Achieving adequate control can be difficult because annual bluegrass exhibits high levels of genetic diversity, the ability to germinate year-round, and has a high tendency for evolving herbicide resistance, currently resistant to at least nine different herbicide sites of action. Current management programs rely heavily on herbicides for control; however, frequent use without implementing …


Survey And Prevalence Of Palmer Amaranth Herbicide Resistance In South Carolina, Mitchell Williams Aug 2023

Survey And Prevalence Of Palmer Amaranth Herbicide Resistance In South Carolina, Mitchell Williams

All Theses

Palmer amaranth is a troublesome weed for growers to control, not only due to its aggressive growth characteristics that limit row-crop production, but because of its resistance to different herbicide modes of action. The first case of herbicide resistance in Palmer amaranth was detected in 1989 and has since grown to nine different herbicide classes throughout the United States. New herbicide modes of action have not been developed since the 1980s, so proper stewardship of the remaining modes of action is important for effective control of Palmer amaranth. Increased herbicide resistance from states bordering South Carolina have been reported; therefore, …


Identification And Quantification Of Allelochemicals Associated With Weed Suppression In Sweetpotato, Varsha Varsha May 2023

Identification And Quantification Of Allelochemicals Associated With Weed Suppression In Sweetpotato, Varsha Varsha

Theses and Dissertations

Mississippi ranks among the leading states in sweetpotato production in the US. However, managing weeds in sweetpotato fields presents a significant challenge, and the limited availability of herbicides labeled for use in sweetpotato makes it even more challenging. The widespread and repeated use of herbicides has led to the development of weed resistance. In addition, herbicides are incompatible with the organic production system. Finding sustainable weed management strategies to provide weed control options for organic cultivation and detain the development of herbicide-resistant weeds under conventional crop production is imperative. The natural chemicals released by the plants, also called allelochemicals, can …


Deconstructing The Art Of Physical Weed Control, Jordan W. Parks May 2023

Deconstructing The Art Of Physical Weed Control, Jordan W. Parks

Electronic Theses and Dissertations

Farmers adjust physical weed control (PWC) tools to optimize efficacy based on observations of weeds, the crop, and soil conditions. These many variables make PWC research challenging. To study PWC tool settings more closely, we constructed a soil bin with a mobile tool carriage inside of a heated glasshouse. The soil bin was 2 m wide by 12 m long by 1 m high, with a tool carriage that can operate at 0.4 to 19.0 km h-1. Tool angle, spacing, depth, and speed can all be precisely adjusted from the tool carriage and control panel. The goal of …


The Bellarmine Bee Bed: Organizing A Native Plant Garden Using Feedback From The Local Community, Kate Moran Apr 2023

The Bellarmine Bee Bed: Organizing A Native Plant Garden Using Feedback From The Local Community, Kate Moran

Undergraduate Theses

Animal pollinators are the cornerstone of healthy ecosystems. Their survival is essential for the persistence of entire food chains: from the flowers they cross-pollinate directly, to the animals who depend on those plants for nutrition. The establishment of pollinator gardens—particularly ones that consist of native plants—is an effective way to enhance their biodiversity, abundance, and well-being.

The main goal of this thesis is to construct a pollinator garden that maximizes the benefits for animal pollinators using feedback from local gardeners. A survey was used to gather information about the popularity and preferences of 40 flowering plants, and after analyzing the …


Louisiana Resident Perceptions On Invasive Aquatic Plant Species And Their Management, Anna Ribbeck Mar 2023

Louisiana Resident Perceptions On Invasive Aquatic Plant Species And Their Management, Anna Ribbeck

LSU Master's Theses

Determining the public perception of invasive species in Louisiana is an important tool to help shape and develop future management decisions, educational material and ultimately public policy. Therefore, the primary purpose of this study was to determine the perceptions of adult Louisiana residents regarding invasive aquatic plant species and their management. An online survey was used to collect data on registered Louisiana residential boaters’ perceptions towards invasive aquatic plants and their management. A postcard with a quick response code leading to the survey was mailed to 6,000 randomly selected Louisiana registered residential boaters. Of the 230 Louisiana registered boaters that …


Belowground Plant Communities: Seed Bank Dynamics And Relationship To Aboveground Vegetation In Grazing Allotment Of Klamath County, Oregon, Dellaina C. Morse, Scott M. Estepa Jan 2023

Belowground Plant Communities: Seed Bank Dynamics And Relationship To Aboveground Vegetation In Grazing Allotment Of Klamath County, Oregon, Dellaina C. Morse, Scott M. Estepa

Environmental Science & Management Senior Capstones

Seed banks are valuable resources for restoration but are understudied. The species present in the aboveground composition influence the seed bank through seed inputs. Land managers utilize herbicides to selectively reduce certain species in the above- and belowground composition. The aim of this paper is to observe and assess the species present in a seed bank post-herbicide application, and to discuss the seed bank potential for future restoration at the specific site in Klamath County, Oregon. Soil core samples were collected in the field and brought to the lab where they were processed and prepared for germination. We watered and …


Forage Grass Growth Habit And Allelopathy In Birdsfoot Trefoil Germination And Establishment, Nathan El Blake Jan 2023

Forage Grass Growth Habit And Allelopathy In Birdsfoot Trefoil Germination And Establishment, Nathan El Blake

Graduate Theses, Dissertations, and Problem Reports

Birdsfoot trefoil (Lotus corniculatus L.) (BFT) is a high quality, tanniniferous forage legume that has been shown to mitigate gastrointestinal nematode (GIN) infection in sheep. Reliable establishment of birdsfoot trefoil in existing pastures is difficult due to BFT’s poor seedling vigor, susceptibility to crown and root rot, and, perhaps chiefly, competition from other pasture plants. Most existing pastures in the Northeastern United States are primarily composed of cool-season grasses, many of which can be highly competitive. A common form of interplant competition is allelopathy, which occurs when secondary metabolites produced by a plant inhibit a necessary growth process of …


Characterization And Management Of Auxin-Resistant Palmer Amaranth In Tennessee, Delaney C. Foster Dec 2022

Characterization And Management Of Auxin-Resistant Palmer Amaranth In Tennessee, Delaney C. Foster

Doctoral Dissertations

Palmer amaranth has a long history of evolving resistance to herbicides to the point it has become a significant row crop production obstacle. Cotton and soybean growers were offered new technologies in 2016, expanding in-crop herbicide options to include dicamba or 2,4-D. Within three years of commercialization, dicamba use in these crops increased ten-fold and growers began to report Palmer amaranth escapes in west Tennessee auxin-tolerant production systems. A survey of Palmer amaranth escapes in dicamba and 2,4-D-tolerant cotton and soybean fields in Tennessee was conducted in the fall of 2021 with the objective of determining if poor control was …


Effects Of Disturbance (Mowing) On Florivory And Floral Defenses In Solanum Elaeagnifolium, A Noxious And Worldwide Invasive Weed, Alejandro Rafael Vasquez Marcano Dec 2022

Effects Of Disturbance (Mowing) On Florivory And Floral Defenses In Solanum Elaeagnifolium, A Noxious And Worldwide Invasive Weed, Alejandro Rafael Vasquez Marcano

Theses and Dissertations

Chapter 1: Literature Review- In this literature review we have examined weed management as it concerns urban and agricultural ecosystems, as well as the need for mowing assessments as it relates to floral traits and defense against herbivory.

Chapter 2: Experiments- In the experiments we examined the role of mowing on floral growth traits, floral defense traits, and their effect on Manduca sexta specialist herbivores.

Chapter 3: Results- In the results we found SLN flowers to have larger diameters than unmowed flowers; however, flower mass was in line with our predictions as unmowed flowers were heavier in high mowing frequency …


Flora Of Doe Mountain Recreation Area, Johnson County, Tennessee, Benjamin Mccullough Aug 2022

Flora Of Doe Mountain Recreation Area, Johnson County, Tennessee, Benjamin Mccullough

Electronic Theses and Dissertations

A botanical inventory of Doe Mountain Recreation Area (DMRA) in northeastern Tennessee was conducted to help guide conservation-based management. A total of 484 species were found in DMRA, comprising 94 families, and 285 genera, 10 species listed in the state rare plant list, and 76 exotic species. Two species, Liatris virgata and Lycopodiella inundata, were new state records. Water in the Lycopodiella seep was an order of magnitude more acid than at other sites. An analysis of the wildland-urban interface showed that only 13% of the area was classified as uninhabited. The inventory-invasion index, introduced to quantify the relative …


Evaluating Cover Crops To Determine The Best Management Practice For The Suppression Of Tall Waterhemp And Italian Ryegrass, Samuel R. Reeves May 2022

Evaluating Cover Crops To Determine The Best Management Practice For The Suppression Of Tall Waterhemp And Italian Ryegrass, Samuel R. Reeves

Theses and Dissertations

To combat herbicide resistance among weeds, non-herbicide methods of control, such as cover crops, are becoming widely adapted. Experiments were conducted to determine how to effectively establish and manage cover crops in order to suppress tall waterhemp and Italian ryegrass and to assess their overall impact on soybean growth and yield. Various cover crop establishment methods were evaluated, and it was determined that interseeding at the R7 growth stage of soybean was the least effective method for proper cover crop establishment. Biomass data demonstrated that interseeding created the least amountof cover crop biomass, with no differences found among the other …


Evaluation Of Agronomic Performance And Weed Control In Soybean Grown With Different Row Configurations, Planting Dates, And Soil Textures, Franklin Read Kelly May 2022

Evaluation Of Agronomic Performance And Weed Control In Soybean Grown With Different Row Configurations, Planting Dates, And Soil Textures, Franklin Read Kelly

Theses and Dissertations

Planting date, seeding rate, soil texture, and row configuration are important factors in soybean production. Each of these factors can impact overall production and yield of soybean immensely. Growers can have difficulty making decisions about how to best manage their production systems with these factors in mind. Therefore, research was conducted from 2019 to 2021 at the Delta Research and Extension Center in Stoneville, MS, to evaluate the agronomic performance, yield components, and weed control of soybean planted with different planting dates, row configurations, soil textures and/or seeding rates. Common row configurations utilized in Mississippi soybean production were compared to …


Restoration Through Reassembly: Evaluating The Role Of Native Plants In Combatting Chinese Tallow, Olaniyi Ajala May 2022

Restoration Through Reassembly: Evaluating The Role Of Native Plants In Combatting Chinese Tallow, Olaniyi Ajala

Electronic Theses and Dissertations

Non-native, invasive species disrupt ecological processes and functions, posing a serious threat to natural ecosystems. By examining the growth metrics of the non-native, invasive tree, Chinese tallow (Triadica sebifera [L.] Roxb.), hereafter tallow, across different flooding and light regimes, I investigated how restructuring native communities with valuable native species will prevent the reestablishment of tallow. I studied the changes in morphological and physiological traits of tallow when growing with water tupelo (Nyssa aquatica L.), sugarberry (Celtis occidentalis L.), and green ash (Fraxinus pennsylvanica Marshall). I found that in the non-flooded and high irradiance treatments, tallow's growth …


Poa Annua: An Annual Species?, Devon E. Carroll May 2022

Poa Annua: An Annual Species?, Devon E. Carroll

Doctoral Dissertations

Poa annua L. is ranked the most troublesome turfgrass weed but can also be a highly desirable turfgrass species. As the Latin name annua implies, the species is thought to persist via an annual life cycle; yet there are many reports in literature of P. annua persisting perennially. Considering that P. annua senescence patterns do not align with other true annual species such as Triticum spp. and Zea mays L., we hypothesized that P. annua presents itself similarly to other perennial, cool-season, turfgrass species that are subject to a confluence of environmental factors in summer that can cause mortality. …


Evaluation Of Cotton Management Decisions: Cover Crops, Weed Control, And Injured Stands, Savana D. Denton May 2022

Evaluation Of Cotton Management Decisions: Cover Crops, Weed Control, And Injured Stands, Savana D. Denton

Doctoral Dissertations

Herbicide-resistant weed species have altered the challenges faced by Tennessee cotton (Gossypium hirsutum, L.) producers. While the weed control and environmental benefits of cover crops have been well-documented, the integration of cover crops into cotton production systems has presented management challenges. In-season broadcast postemergence weed control options are limited in cotton. Furthermore, off-target movement of 2,4-D and dicamba can result in additional management challenges if susceptible cotton is injured. Studies were conducted from 2018 to 2021 to evaluate: 1) cotton response to cover crop termination timings and methods; 2) postemergence weed control programs in cotton without the use of …


Effect Of Herbicide Concentration On Atrazine, Pyroxasulfone, And Saflufenacil Dissipation Under Field And Lab Conditions, Randall Lee Landry May 2022

Effect Of Herbicide Concentration On Atrazine, Pyroxasulfone, And Saflufenacil Dissipation Under Field And Lab Conditions, Randall Lee Landry

Doctoral Dissertations

Widespread herbicide resistant weeds over the last several years have fueled a renewed interest in the utility of soil-applied residual herbicides. Residual activity of a herbicide is based on a myriad of factors within and among environments. A common assumption used in simulation models examining herbicide persistence is that herbicide degradation is independent of application rate. These studies compared herbicide concentration on dissipation with three commonly used residual herbicides under field and lab conditions. Atrazine, pyroxasulfone, and saflufenacil represent herbicides with a relative field persistence of medium, high, and low, respectively. Field studies were conducted over two years where herbicides …