Life Sciences Commons^™

Phenotypic And Genotypic Characterization Of Antimicrobial Resistance In Commonly Isolated Salmonella Serovars From Chickens, Seyyi̇de Sariçam İnce, Mehmet Akan

Turkish Journal of Veterinary & Animal Sciences

Salmonellosis caused by Salmonella agents is the second most common zoonotic infection in humans. In recent years, Salmonella's increasing antimicrobial resistance (AMR) has been a concern. The major transmission route of Salmonella is consumption of contaminated poultry products. Therefore, monitoring of antimicrobial resistance in chicken-originated Salmonella is critically important. This study investigated AMR in four commonly isolated Salmonella serovars from chickens, namely Salmonella Enteritidis (S. Enteritidis), Salmonella Infantis (S. Infantis), Salmonella Kentucky (S. Kentucky), and Salmonella Typhimurium (S. Typhimurium). A total of 133 isolates were examined by phenotypic and genotypic AMR characterization. Resistance to 14 different antimicrobials and eight …

Evaluating The Microbial Quality And Use Of Antimicrobials In Raw Pet Foods, Leslie Pearl Cancio

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Raw meat-based diets (RMBDs) are emerging pet foods that pose food safety risks because of the potential presence of pathogens that could cause illness to humans. In this research, the microbial quality of select RMBD products sold by pet food companies online and the use of chemical antimicrobials to reduce the microbial load in chicken liver, a common RMBD ingredient, were evaluated.

Ground meat blends and livers from four animal species (beef, pork, chicken, turkey) were purchased from four online companies that delivers directly to consumers through parcel businesses. Products were procured at three different times during one year and …

Occurrence Of Some Pathogenic Bacteria In Cattle Feed Samples Before And After Invasionby European Starlings, Özcan Şahi̇n, Talha Demi̇rci̇, Sai̇m Boztepe, İbrahi̇m Ayteki̇n, İsmai̇l Keski̇n, Uğur Zülkadi̇r

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to investigate the species identification and enumeration of some pathogenic microorganisms before and after feeding the full ration contaminated by predatory starling bird herds on 5 different days in the dairy cattle farm in Aksaray. At the end of the study, the average number of E.coli obtained from the feed samples taken before the arrival of starlings was 6.46 log cfu/g, while the number of E.coli obtained from the feed taken after starling birds flocked to the feed was found as 6.80 log cfu/g. While the average Campylobacter spp. number was 5.50 log cfu/g, this …

Typing Of Rarely Isolated Salmonella Serotypes With Mlst, Seyyi̇de Sariçam İnce, İnci̇ Başak Müştak, Hami̇t Kaan Müştak

Turkish Journal of Veterinary & Animal Sciences

Salmonellosis caused by nontyphoidal Salmonella is the second most common zoonotic infection worldwide. The major foodborne route of this infection is the consumption of contaminated poultry meat and egg. Therefore, monitoring and control of Salmonella agents in poultry populations is critical to reduce the risks for public health. In the poultry industry, the most common serotypes in Salmonella infections are Salmonella enterica subsp. enterica serotype Enteritidis and Typhimurium. In recent years, other frequently isolated Salmonella serotypes have also increased in Turkey because of the Salmonella control programs regulated by many countries. Hence, other Salmonella serotypes (except for the most frequently …

Typing Of Φsp-3 Lytic Salmonella Bacteriophages Obtained From Various Fecal Sources, Asli Sakmanoğlu, Hasan Hüseyi̇n Hadi̇mli̇

Turkish Journal of Veterinary & Animal Sciences

Although several reports are available on both ΦSP-1 and ΦSP?3 lytic Salmonella bacteriophages obtained from poultry, further research is required to study the effectiveness of ΦSP-3 type on serovars isolated from other sources. In the present study, we aimed to isolate bacteriophages from 8 serovars previously obtained from 869 fecal samples (calf, dairy cow, buffalo, and camel), genotype the bacteriophages, and detect the cross-lytic activities of the bacteriophages on Salmonella enterica subsp. enterica serovar Kentucky, S.Anatum, and S.Muenchen. A total of 16 bacteriophages were detected as ΦSP-3 type via PCR. The Hunter-Gaston Discriminatory Index of SDS-PAGE was calculated to be …

Base Study For The Establishment Of National Salmonella Control Program In Hatching Farms And Table Eggs In Turkey, Kadi̇r Serdar Di̇ker, Muammer Göncüoğlu, Güzi̇n Şahi̇n, Mehmet Akan, İsmayi̇l Safa Gürcan, Hami̇t Kaan Müştak, Nai̇m Deni̇z Ayaz, Seyyi̇de Sariçam, Merve Özdal Salar, Hafi̇ze Di̇lşad Açikalin, Gülteki̇n Ünal, Fethi̇ye Çöven, Asi̇ye Dakman, İrem Gülaçti, Emi̇ne Nazan Uzunboy

Turkish Journal of Veterinary & Animal Sciences

Foodborne infections due to Salmonella are still a major concern worldwide. Particularly contaminated egg and egg related products are the primary sources for human salmonellosis. It is necessary to determine the risk factors associated with Salmonella contamination of eggs within the scope of farm to table and environment. The objective of this study was to develop the "National Salmonella Control Program in Laying Hens" and report the prevalence and serotype distribution findings of Salmonella in laying hens and eggs in Turkey. A total of 2122 samples were collected and analysed according to ISO 6579:2002 after the isolation and identification procedures. …

Isolation And Molecular Characterization Of Salmonella Enterica And Escherichia Coli From Poultry Samples, Ayli̇n Cesur, Saci̇de Özlem Ulutaş, Yeşi̇m Soyer

Turkish Journal of Veterinary & Animal Sciences

Innumerable foodborne pathogens including Salmonella and Escherichia coli pose a serious threat to human health and food safety. As perishable foods, poultry products are considered one of the most common sources of foodborne pathogens including Salmonella and E. coli due to transmission of drug resistance, dissemination of organisms, and cross-contamination. In our study, phenotypic and genotypic characterizations of Salmonella enterica and E. coli isolated from packaged raw chicken products were carried out. Samples belonging to different commercial brands were collected in Ankara in 2015. Among 15 out of 19 Salmonella enterica subsp. enterica strains isolated from different and/or same poultry …

Quantitative Microbial Risk Assessment For Parts, Ground, And Msc Poultry Product Including Intervention Analysis And Exploration Of Enterobacteriaceae As An Indicator Organism In Poultry Processing, Leigh Ann Parette

Graduate Theses and Dissertations

Samples collected at five different large bird poultry processing facilities over a period of 7 months from prescald to post debone locations were enumerated for Enterobacteriaceae, Salmonella spp., and Campylobacter spp. and the results were used to create Quantitative Microbial Risk Analyses (QMRA) models for parts, ground, and mechanically separated chicken (MSC) products. Sensitivity analyses indicated the points in the process at which reductions would be most advantageous to the endpoint and simulation models were run to test reductions required to meet the current USDA performance standards.

These data were analyzed to determine the reductions from one node (location) to …

Serotypes Of Salmonella Isolated From Feces Of Cattle, Buffalo,And Camel And Sensitivities To Antibiotics In Turkey, Hasan Hüseyi̇n Hadi̇mli̇, Yasemi̇n Pinarkara, Asli Sakmanoğlu, Zafer Sayin, Osman Ergani̇ş, Ali̇ Uslu, Huda Jihad Al-Shattrawi

Turkish Journal of Veterinary & Animal Sciences

In this study, Salmonella strains from dairy cows, calves with diarrhea, and buffalo and camel feces were isolated and serotyped. There were 869 feces samples (437 calves, 287 dairy cows, 100 buffalo, and 45 camels) collected from 13 provinces and 21 farms. Preenrichment feces samples were added to Rappaport-Vassiliadis medium. Samples were then subcultured on xylose lysine tergitol-4 agar and plates. Suspected colonies of Salmonella were confirmed by latex agglutination test. The isolates of Salmonella spp. were serotyped at the Etlik Central Veterinary Control Institute. In total, 40 Salmonella spp. were isolated, including 33 from calves, 5 from dairy cows, …

The Protective Efficacy Of Immunoglobulin Y From Immunized Chickens Against Salmonella Infections In Mice, Hasan Hüseyi̇n Hadi̇mli̇, Zafer Sayin, Gökçenur Sani̇oğlu Gölen

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the efficacy of immunoglobulin Y (IgY) obtained from chickens immunized with Salmonella vaccines. Chickens were vaccinated three times with inactivated monovalent, bivalent, and combined vaccines. Immunized hen eggs were collected after the third vaccination and IgYs were purified. In total, 100 mice were orally challenged with Salmonella serotypes. After the challenge, IgYs were orally administered to mice. Mice were observed for morbidity and mortality. Fecal samples from the mice were also cultured for the reisolation of Salmonella serotypes. The antibody titers in the serum samples of vaccinated chickens were higher than those …

Occurrence And Antimicrobial Resistance Of Salmonella Enterica Subsp. Enterica Serovars Typhimurium, Enteritidis, And Typhi Isolated From Chicken Eggs And Poultry Products, Serhat Al, Harun Hizlisoy, Nurhan Ertaş Onmaz, Yeli̇z Yildirim, Zafer Gönülalan

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to detect the occurrence and antimicrobial resistance of Salmonella enterica subsp. enterica serovars Typhimurium, Enteritidis, and Typhi in 252 samples including 100 chicken eggs, 60 processed poultry products (20 nuggets, 20 salami samples, 20 sausages), and 92 poultry giblets (50 livers and 42 gizzards). Salmonella spp. and serovars were identified by PCR. The antimicrobial susceptibility testing was performed by disk diffusion method. Forty-seven (31%) of 152 poultry products and 5 (5%) of 100 egg samples were positive for Salmonella spp. Salmonella spp. was positive in 3.3%, 5%, 18%, and 27% of chicken nugget, sausage, gizzard, …

The Human/Animal Interface: Emergence And Resurgence Of Zoonotic Infectious Diseases, Michael Greger

Michael Greger, MD, FACLM

Emerging infectious diseases, most of which are considered zoonotic in origin, continue to exact a significant toll on society. The origins of major human infectious diseases are reviewed and the factors underlying disease emergence explored. Anthropogenic changes, largely in land use and agriculture, are implicated in the apparent increased frequency of emergence and reemergence of zoonoses in recent decades. Special emphasis is placed on the pathogen with likely the greatest zoonotic potential, influenzavirus A.

A Molecular Study Of The Salmonella Enterica Serovars Abortusovis, Typhimurium, And Enteritidis, Taghi Zahraei Salehi, Omid Madadgar, Hasan Tadjbakhsh, Mohammad Reza Mahzounieh, Mohammad Mehdi Feizabadi

Turkish Journal of Veterinary & Animal Sciences

This study presents a molecular analysis of 3 important human and animal serovars of Salmonella: Typhimurium, Enteritidis, and Abortusovis. We also provide information that can be applied in the surveillance of salmonellosis. Over the course of 3 decades, 90 isolates were collected, with 30 isolates representing each type of serovar. In addition to conventional serotyping, serovars were confirmed by polymerase chain reaction (multiplex PCR and IS200 PCR). Pulsed-field gel electrophoresis (PFGE) of the serovars confirmed 12, 15, and 20 patterns, respectively. In an analysis of the results, 5 pulsotypes (80% of isolates) of Salmonella Typhimurium and 4 pulsotypes (64% of …

Identification And Characterization Of Salmonella Isolates From Captured House Sparrows, Sara Mirzaie, Mohammad Hassanzadeh, Iradj Ashrafi

Turkish Journal of Veterinary & Animal Sciences

The objectives of this study were to determine the prevalence of Salmonella in a population of house sparrows, which are commonly found around poultry houses, and to characterize the obtained Salmonella isolates via serotyping, multiplex polymerase chain reaction (PCR), and antibiotic resistance analysis. Samples of visceral organs (gastrointestinal tract, liver, and heart) from 470 house sparrows were subjected to culture and the results show that 18 (3.8%) were positive for Salmonella. Of the 18 Salmonella isolates characterized, the most predominant serovars were Salmonella Typhimurium and S. Enteritidis (9 and 8 cases each, respectively), whereas only 1 serovar belonged to S. …

The Human/Animal Interface: Emergence And Resurgence Of Zoonotic Infectious Diseases, Michael Greger

Transgenesis Collection

Emerging infectious diseases, most of which are considered zoonotic in origin, continue to exact a significant toll on society. The origins of major human infectious diseases are reviewed and the factors underlying disease emergence explored. Anthropogenic changes, largely in land use and agriculture, are implicated in the apparent increased frequency of emergence and reemergence of zoonoses in recent decades. Special emphasis is placed on the pathogen with likely the greatest zoonotic potential, influenzavirus A.

Prevalence Of Salmonella Serogroups In Chicken Meat, Gülşen Goncagül, Elçi̇n Günaydin, K. Tayfun Çarli

Turkish Journal of Veterinary & Animal Sciences

The purpose of this study was to investigate the prevalence of Salmonella serogroups in chicken meat. A total of 315 skins from the wing part of chicken carcasses were collected from 8 chicken carcass retailers. Salmonella isolation was performed as described in the Bacteriological Analytical Manual of the Food and Drug Administration, Center for Food Safety and Applied Nutrition. Salmonella enterica subsp. enterica Serovar Enteritidis (Salmonella Enteritidis) in Serogroup D was isolated from 27 out of 315 (8.57%) chicken carcass skins from the wing parts. Four out of 45 (8.88%), 3 out of 50 (6.00%), 12 out of 57 (21.05%), …

Microbiological Quality And Chemical Composition Of The Döner, Vi̇ldan Küpeli̇ Gençer, Mükerrem Kaya

Turkish Journal of Veterinary & Animal Sciences

Chemical composition and microbiological quality of 40 döner samples (cooked) taken from 8 different restaurants in Erzurum city center were investigated. Total aerobic bacteria count was an average of 5.1 log CFU/g. Lactic acid bacteria counts in 20% of samples, Enterobacteriaceae and S. aureus counts in 60% of samples, coliform bacteria counts in 45% of samples, E. coli counts in 67.5% of samples, and C. perfringens count in 85% of samples were under detectable levels (

Farm Disease Crises In The United Kingdom: Lessons To Be Learned, Michael C. Appleby

State of the Animals 2003

Over the last fifteen years, a diverse succession of disease-related crises has befallen farm animal and food industries in the United Kingdom. Some have involved animal health, with little risk to humans. Some have involved human health, with animals acting as a reservoir for infection but little affected themselves. Some, however— including the most alarming— have involved both animal and human health through zoonoses, diseases transmittable from animal to human. All of these crises are linked in the public mind and in many commentaries, and indeed there are issues that many of them share. The most common of these is …

Identification Of Salmonella Isolated From Freshwater Mussels (Unio Elongatulus Eucirrus Bourguignat, 1860) By Polymerase Chain Reaction, Engi̇n Şeker, Mustafa Sarieyyüpoğlu, Burhan Çeti̇nkaya

Turkish Journal of Veterinary & Animal Sciences

In this study, 627 live freshwater mussels collected from the Koçkale and Pertek regions of Keban Dam Lake were examined for the presence of Salmonella spp. Homogenates prepared from the soft tissue of mussels, after pre-enrichment in Rappaport Vasilliadis, were inoculated onto Brilliant Green Phenol Red Lactose agar and Salmonella-Shigella selective media. Biochemical tests and DNA extraction were carried out on isolates suspected of containing Salmonella. From 19 (4.8%) out of 397 mussels collected in the Koçkale region, Salmonella spp. were isolated and identified, but the agent could not be detected in mussels obtained from the Pertek region. DNA extracted …

Salmonella Infection In A Chinchilla Farm, Deni̇z Misirlioğlu, Cengi̇z Çeti̇n, M. Müfi̇t Kahraman, Vi̇ldan Caner, M. Özgür Özyi̇ği̇t

Turkish Journal of Veterinary & Animal Sciences

This study describes a salmonella infection in a chinchilla farm of 40 families in the Yalova region. Twenty-four families were affected in a 10-day period after the outbreak of the disease and the clinical signs were apathy, anorexia, diarrhoea with or without hemorrhage, tremors, local paralysis, mucopurulent or hemorrhagic vaginal secretion in some females, abortions in 1 to 2 month-pregnant animals and death. The newborn and pregnant animals were affected more severely. Two live chinchillas with clinical signs were euthanized and gross pathological, histopathological and microbiological examinations were performed. In the gross and microscopical examinations, the most severe lesions were …

Rapid Identification Of Avian Salmonella Isolates By Air Thermal Cycler Amplification And Capillary Gel Electrophoresis, K. Tayfun Çarli, Vi̇ldan Caner, Ayşegül Eyi̇gör

Turkish Journal of Veterinary & Animal Sciences

The possible use of a genus-specific polymerase chain reaction (PCR) for the identification of Salmonella colonies was tested on 14 reference strains and 38 clinical Salmonella strains isolated from chickens by the standard method of the National Poultry Improvement Plan and Auxiliary Provisions (NPIP), USDA, U.S.A. All the PCRs using primer pairs InvA1 and InvA2 (5) gave 281 bp amplification product specific for the Salmonella genus and produced results identical (100%) to those obtained with biochemical and serological methods. Thus, the genus-specific capillary PCR for the identification of a colony of Salmonellae from a selective agar plate was performed within …

A Study On The Effects Of Chilling On The Microbiological Quality Of Poultry Carcasses And Surface Decontamination With Lactic Acid, Hali̇l Tosun, Abdurrahman Üsame Tamer

Turkish Journal of Veterinary & Animal Sciences

Effects of immersion chilling on the microbiological quality of poultry carcasses and cross contamination of Salmonella were studied. Levels of aerobic mezophilic bacteria, coliform, Escherichia coli and the incidence of Salmonella were determined prechill and postchill. The level of coliform and E. coli were reduced by immersion chilling (P0.05) but there was an increase in Salmonella incidence in carcasses exiting the immersion chiller indicating that this may be the point of most significant cross contamination in broiler processing plants. Experiments on microbial decontamination of poultry carcasses with lactic acid were performed. The microbial levels of poultry carcasses were reduced significantly …

Typing Of Salmonella Spp. Isolated From Chickens In Elazığ Region, Hakan Kalender, Adi̇le Muz

Turkish Journal of Veterinary & Animal Sciences

In this study, 365 chickens slaughtered at a local abattoir and 162 chickens admitted to Elazığ Veterinary Control and Research Institute were investigated for Salmonellosis. Salmonella strains were identified and serogrouped. Antibiotic susceptibility tests were also performed. Fifty seven (10.81%) Salmonella strains were isolated from 527 chickens. Salmonella strains were serogrouped by coagglutination test and slide agglutination test. Of 57 strains, 53 were in group D1, 4 were in group B. Of 53 strains in group D1, 39 were serotyped as S. enteritidis and 14 as S. gallinarum. Four strains in group B were serotyped as S. typhimurium. In phagetyping …

Incidence And Serotipe Distribution Of Salmonella In Ground Beef In Ankara, İrfan Erol

Turkish Journal of Veterinary & Animal Sciences

The present study was conducted to determine of incidence and serotype distribution of salmonellae in ground beef on markets in Ankara. Between February 1994 and January 1995, a total of 120 samples were obtained from different butcheries and supermarkets of different regions in Ankara. Salmonellae were found in 3.3% (4/100) of ground beef samples. Three different serotypes were identified among the cultures isolated namely S. anatum (2), S. typhimurium (1) and S. telaviv (1). The incidence of salmonellae was found high in the period of June-September months. While three of salmonellae positive samples belonged to supermarkets, onli one Salmonella positive …

Salmonella Infection In Farm Animals, F C. Wilkinson

Journal of the Department of Agriculture, Western Australia, Series 4

FIFTY-EIGHT cases of salmonella infection were diagnosed in farm animals in Western Australia during 1965.

Losses, due to deaths, abortion and ill-thrift on properties where these cases occurred were often substantial and caused the owner financial hardship.

Many more outbreaks of salmonellosis undoubtedly occurred but were not brought to the notice of veterinarians.