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Full-Text Articles in Life Sciences
Mva I Pcr-Rflp Identifies Single Nucleotide Polymorphism Of The Alpha-Lactalbumin Gene In Some Goat Breeds Reared In Turkey, Özgecan Korkmaz Ağaoğlu, Mustafa Saatci, Özkan Elmaz, Mehmet Çolak, Mesi̇h Kocamüftüoğlu, Emel Zeytünlü
Mva I Pcr-Rflp Identifies Single Nucleotide Polymorphism Of The Alpha-Lactalbumin Gene In Some Goat Breeds Reared In Turkey, Özgecan Korkmaz Ağaoğlu, Mustafa Saatci, Özkan Elmaz, Mehmet Çolak, Mesi̇h Kocamüftüoğlu, Emel Zeytünlü
Turkish Journal of Veterinary & Animal Sciences
Alpha-lactalbumin plays a key role in lactose synthesis in the mammary glands of domestic animals. This study evaluated Hair (188), Saanen (82), Kilis (63), and Honamli (183) goat breeds, with the primary goal of investigating DNA polymorphism of Mva I RFLP at exon 3 of the alpha-lactalbumin gene. Polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) was employed to genotype a total of 516 goats for Mva I polymorphism of alpha-lactalbumin. In the breeds that were studied, digestion of the amplification product with the Mva I restriction enzyme revealed 2 alleles (A1 and A2) and 3 genotypes (A1A1, A1A2, …
In Vivo Embryo Production In Some Native Goat Breeds In Turkey, Ali̇ Reha Ağaoğlu, Kübra Karakaş, Mustafa Kaymaz
In Vivo Embryo Production In Some Native Goat Breeds In Turkey, Ali̇ Reha Ağaoğlu, Kübra Karakaş, Mustafa Kaymaz
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to assess the efficacy of the repeated administration of superovulatory protocol and surgical procedure in in vivo embryo production, as well as to determine the differences in the responses to superovulatory treatment among several Turkish native goat breeds (Angora, Kilis, Honamli, Hair, and Norduz). A superovulatory treatment procedure was repeated 3 times in this study. The estrus cycles were synchronized and superovulatory response was induced with porcine follicle-stimulating hormone during the natural breeding season. Natural mating was performed following CIDR removal. Embryo recovery was performed surgically on day 6, after the first mating. According …
The Effects Of Lh On Progesterone Production By Cell Subpopulations Isolated From Early And Late Luteal Phase Goat Corpora Lutea, Hakan Kalender, Şevket Arikan, Özkan Şi̇mşek
The Effects Of Lh On Progesterone Production By Cell Subpopulations Isolated From Early And Late Luteal Phase Goat Corpora Lutea, Hakan Kalender, Şevket Arikan, Özkan Şi̇mşek
Turkish Journal of Veterinary & Animal Sciences
The objective of this study was to investigate the complementary effects of luteinizing hormone (LH) on cell subpopulations. Corpora lutea were collected on days 5 and 15 of the cycle. The luteal tissues were treated with collagenase for cell dissociation. Percoll density-gradient centrifugation was performed to separate cells into 2 distinct subpopulations. Luteal cells (5 × 104 cells/well) were cultured with and without 22(R)-hydroxycholesterol (22R-HC, 10 µg/mL) and LH (10 and 100 ng/mL) alone, and also with 22R-HC (10 µg/mL) plus LH (10 and 100 ng/mL), in a serum-free culture medium for 6 days of incubation. Untreated cells isolated from …
Screening For S32g Mutation Of Bmp15 Gene In 18 Goat Breeds, Tao Feng, Ming-Xing Chu, Gui-Ling Cao, Dong-Wei Huang, Ran Di, Qiu-Yue Liu, Zhang-Yuan Pan, Mei Jin, Ying-Jie Zhang
Screening For S32g Mutation Of Bmp15 Gene In 18 Goat Breeds, Tao Feng, Ming-Xing Chu, Gui-Ling Cao, Dong-Wei Huang, Ran Di, Qiu-Yue Liu, Zhang-Yuan Pan, Mei Jin, Ying-Jie Zhang
Turkish Journal of Veterinary & Animal Sciences
In our early work, one mutation (A898G, which caused Ser32Gly (S32G) change of mature peptide) was identified in the goat BMP15 gene and the allele G was associated with high litter size in Jining Grey goats (the goat breed with the highest fecundity in China). The aim of this research was to investigate the genetic structure of the prolific BMP15 gene in 18 goat breeds reared in China, including 12 native breeds, 3 introduced breeds, and 2 cultivated breeds. Genomic DNA of 1011 goats was screened for the S32G mutation. The results showed that this mutation existed in all 3 …
Polledness Intersex Syndrome In Goats – Molecular And Histological Aspects, Iwona Szatkowska, Daniel Zaborski, Witold Stanislaw Proskura, Sara Tabor
Polledness Intersex Syndrome In Goats – Molecular And Histological Aspects, Iwona Szatkowska, Daniel Zaborski, Witold Stanislaw Proskura, Sara Tabor
Turkish Journal of Veterinary & Animal Sciences
Polledness intersex syndrome (PIS) is a phenomenon associated with 2 distinct conditions occurring in goats, namely polledness and intersexuality. The first is a trait exhibiting complete dominance, whereas the latter is recessive. The extreme case of intersexualism is complete sex reversal, which is observed only in females (60,XX). The trait PIS is inherited in a recessive manner and caused by deletion of 11.7 kbp DNA on chromosome 1q43. Female goats with PIS exhibit a broad range of developmental abnormalities of the sexual system, from only subtle changes to complete sex reversal. The phenotypic diversity in goats with PIS is most …
New Understanding Of An Old Phenomenon: Uncontrolled Factors And Misconceptions That Cast A Shadow Over Studies Of The ‘Male Effect’ On Reproduction In Small Ruminants, Trina Jorre De St Jorre, Penny Alison Rhian Hawken, Graeme Bruce Martin
New Understanding Of An Old Phenomenon: Uncontrolled Factors And Misconceptions That Cast A Shadow Over Studies Of The ‘Male Effect’ On Reproduction In Small Ruminants, Trina Jorre De St Jorre, Penny Alison Rhian Hawken, Graeme Bruce Martin
Turkish Journal of Veterinary & Animal Sciences
In female sheep and goats, exposure to males can be used to induce ovulation and thus control and synchronise fertility. This 'male effect' offers simple, cost-effective, and hormone-free management of reproduction in flocks and herds but, despite decades of research, significant gaps in our knowledge impede commercial application. Many of these gaps can be traced back to misconceptions in the early documentation of the male effect, and others are the result of uncontrolled factors in experimental design. Consequently, it is often difficult to draw conclusions from past research, to further our understanding of the phenomenon, and to make recommendations for …