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Full-Text Articles in Life Sciences
Arginase Occurrence, Development And Distribution In Various Organs Of Rainbow Trout (Oncorhynchus Mykiss), Sema Temi̇zer Ozan, Mi̇ne Eri̇şi̇r, Nai̇m Sağlam
Arginase Occurrence, Development And Distribution In Various Organs Of Rainbow Trout (Oncorhynchus Mykiss), Sema Temi̇zer Ozan, Mi̇ne Eri̇şi̇r, Nai̇m Sağlam
Turkish Journal of Veterinary & Animal Sciences
Arginase (L-arginine amidinohydrolase; E.C. 3.5.3.1) enzyme activity levels in sperm, egg, fertilized egg and larvae (up to 25 weeks) of rainbow trout (Oncorhynchus mykiss) taken from Fırat University, Faculty of Fisheries, Cip Fish Breeding Station were observed weekly. When the organs of rainbow trout were dissected under microscope, enzyme distribution in organs were investigated. Arginase activities in sperm, egg, fertilized egg and rainbow trout (up to 3 weeks) were at unmeasurable level. Increase of enzyme activity was observed in fish after third week. Between the three and twenty five weeks of larvae arginase activity levels were between 0.018 ± 0.001 …
Erythrocyte, Saliva And Serum Arginase Activity With Serum Urea And Estrogen Levels Inakkaraman And Ivesi Sheep In Pregnancy And Postpartum, Sema Temi̇zer Ozan, Sema Yaralioğlu Gürgöze, Tülay İleri̇ Büyükoğlu, İhsan Hali̇feoğlu
Erythrocyte, Saliva And Serum Arginase Activity With Serum Urea And Estrogen Levels Inakkaraman And Ivesi Sheep In Pregnancy And Postpartum, Sema Temi̇zer Ozan, Sema Yaralioğlu Gürgöze, Tülay İleri̇ Büyükoğlu, İhsan Hali̇feoğlu
Turkish Journal of Veterinary & Animal Sciences
Arginase (L-arginine amidino hydrolase E.C. 3.5.3.1) is an enzyme catalyzing the last stage of the Krebs-Henseleit cycle and, thus, converting arginine into urea and ornithine. Arginase also plays a part in protein and polyamine synthesis and is affected by hormones in terms of metabolic status. In the present study, erythrocyte, saliva, and serum arginase activity together with serum urea and estrogen levels were examined in Akkaraman and Ivesi sheep in pregnancy and postpartum. Comparisons were then made between the species. In pregnancy the urea level in saliva increased. However, the urea level in serum decreased. No clear difference was observed …
Gsh-Px, G6pd And Arginase Activities And Some Biochemical Parameters In Cattle Infected With Theileria Annuluta, Sema Temi̇zer Ozan, Edi̇p Özer, Sema Yaralioğlu Gürgöze, Seval Yilmaz, Cem Ecmel Şaki̇, Murat Sevgi̇li̇
Gsh-Px, G6pd And Arginase Activities And Some Biochemical Parameters In Cattle Infected With Theileria Annuluta, Sema Temi̇zer Ozan, Edi̇p Özer, Sema Yaralioğlu Gürgöze, Seval Yilmaz, Cem Ecmel Şaki̇, Murat Sevgi̇li̇
Turkish Journal of Veterinary & Animal Sciences
Theileria annuluta, for which Hyalomma tick species serve as intermediate host, causes an economically important disease in cattle 15 cattle infected with T.annulata and 15 healthy cattle were used in the study. The activities of glutathione peroxidase (GSH-Px), glucose 6 phosphate dehydrogenase (G6PD) and arginase that play role in the free radical metabolism in erythrocytes, and serum aspartate transaminase (AST), alanine transaminase (ALT), total billirubin, cholesterol trigliserid, total protein, albumin, urea, uric acid, haemoglobin, haematocrit levels were measured. Erythrocyte GSH-Px and G6PD activities were measured by beutler, arginase activitiy by the modified TDMU method and other serum parameters were evaluated …
Some Properties Of Purified And Non-Purified Rumen Tissue Arginase In Cattle, Mi̇ne Eri̇şi̇r, Sema Temi̇zer Ozan
Some Properties Of Purified And Non-Purified Rumen Tissue Arginase In Cattle, Mi̇ne Eri̇şi̇r, Sema Temi̇zer Ozan
Turkish Journal of Veterinary & Animal Sciences
Some biochemical properties of purified and non-purified rumen tissue arginase were compared. Homogenization, heating, treatment with aceton, precipitation with ammonium sulfate, dialysis, several centrifugations, gel filtration on sephadex G-200 processes were utilized in the purification procedure of the enzyme. It was found that pre-incubation temperature (60 °C) of arginase and Km (4mM) to its substrate, L-arginine, did not change before and after purification. While pre-incubation period was 10 min and optimal pH 9.7 before purification, pre-incubation period was found to be 5 min and optimal pH 10 after purification. Purified enzyme achieved its highest activity at 2 mM MnCl_2 concentration, …