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Characterization Of Fusobacterium Isolates From The Respiratory Tract Of White-Tailed Deer (Odocoileus Virginianus ), Amit Kumar, Dvm, Ms, Phd
Characterization Of Fusobacterium Isolates From The Respiratory Tract Of White-Tailed Deer (Odocoileus Virginianus ), Amit Kumar, Dvm, Ms, Phd
Amit Kumar, DVM, MS, PhD
A total of 23 clinical isolates of Fusobacterium spp. were recovered at necropsy over a 2-year period from the respiratory tract of white-tailed deer (Odocoileus virginianus). Isolates were identified as Fusobacterium varium (18/23),Fusobacterium necrophorum subsp. funduliforme (3/23), and Fusobacterium necrophorum subsp. necrophorum (2/23). Usingpolymerase chain reaction–based detection of virulence genes, all F. necrophorum isolates were positive for the promoter region of the leukotoxin operon and the hemagglutinin-related protein gene, while all F. varium isolates were negative. The presence of the leukotoxin gene in F. necrophorum isolates and the absence of this gene in F. varium isolates were confirmed by Southern …