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Evaluation Of Chicken Heterophil Adherence, Claire B. Andreasen, Kenneth S. Latimer, W. L. Steffens
Evaluation Of Chicken Heterophil Adherence, Claire B. Andreasen, Kenneth S. Latimer, W. L. Steffens
Claire B. Andreasen
Adherence of chicken heterophils was evaluated at 37 C using preconstructed columns containing various weights of nylon fiber (75 mg, 100 mg, or 125 mg) and whole blood anticoagulated with sodium heparin or 10% disodium ethylenediamine tetraacetic acid (EDTA). Additionally, 50-mg and 75-mg nylon fiber columns incubated at 41 C were used to evaluate heterophil adherence at an increased temperature. The mean percent adherence for heparin-anticoagulated blood applied to 75-mg, 100-mg, and 125-mg nylon fiber columns at 37 C was 76%, 92% and 97.4%, respectively. Samples applied to 50-mg and 75-mg columns at 41 C had adherence values of 27% …
Separation Of Turkey Heterophils From Blood Using Two-Step Ficoll-Hypaque Discontinuous Gradients, Kenneth S. Latimer, Ingrid M. Kircher, Claire B. Andreasen
Separation Of Turkey Heterophils From Blood Using Two-Step Ficoll-Hypaque Discontinuous Gradients, Kenneth S. Latimer, Ingrid M. Kircher, Claire B. Andreasen
Claire B. Andreasen
A method is presented to separate turkey heterophils from anticoagulated whole blood using two-step Ficoll-Hypaque discontinuous gradients and ammonium chloride lysis of contaminating erythrocytes. Heterophils can be isolated from multiple blood samples within 3 to 4 hours. Using this technique, 66.4 +- 18.4% (mean +- standard deviation) of blood heterophils were harvested. Final cell isolates averaged 96.0 +- 2.9% heterophils with few contaminating eosinophils (2.5 +- 2.3%) or basophils (1.6 +- 1.8%). Cell viability, as determined by trypan blue dye exclusion, was 98.0 +- 1.4%.
Separation Of Avian Heterophils From Blood Using Ficoll-Hypaque Discontinuous Gradients, Claire B. Andreasen, Kenneth S. Latimer
Separation Of Avian Heterophils From Blood Using Ficoll-Hypaque Discontinuous Gradients, Claire B. Andreasen, Kenneth S. Latimer
Claire B. Andreasen
Rapid separation of avian heterophils from anticoagulated whole blood was achieved using Ficoll-Hypaque discontinuous gradients. An average of 14.4% of blood heterophils was harvested with a mean purity exceeding 99%. Heterophil viability, as determined by trypan blue dye exclusion, averaged 99.8%. The integrity of isolated heterophils was evaluated by cytochemical staining and ultrastructural examination. Cytochemical staining reactions of heterophils in whole blood and of isolated cell suspensions were similar. No ultrastructural abnormalities were observed. Using this procedure, viable intact heterophils were rapidly isolated from blood with an acceptable cell yield and purity for cell function studies.