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Veterinary Medicine

Turkish Journal of Veterinary & Animal Sciences

2011

Salmonella

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A Molecular Study Of The Salmonella Enterica Serovars Abortusovis, Typhimurium, And Enteritidis, Taghi Zahraei Salehi, Omid Madadgar, Hasan Tadjbakhsh, Mohammad Reza Mahzounieh, Mohammad Mehdi Feizabadi Jan 2011

A Molecular Study Of The Salmonella Enterica Serovars Abortusovis, Typhimurium, And Enteritidis, Taghi Zahraei Salehi, Omid Madadgar, Hasan Tadjbakhsh, Mohammad Reza Mahzounieh, Mohammad Mehdi Feizabadi

Turkish Journal of Veterinary & Animal Sciences

This study presents a molecular analysis of 3 important human and animal serovars of Salmonella: Typhimurium, Enteritidis, and Abortusovis. We also provide information that can be applied in the surveillance of salmonellosis. Over the course of 3 decades, 90 isolates were collected, with 30 isolates representing each type of serovar. In addition to conventional serotyping, serovars were confirmed by polymerase chain reaction (multiplex PCR and IS200 PCR). Pulsed-field gel electrophoresis (PFGE) of the serovars confirmed 12, 15, and 20 patterns, respectively. In an analysis of the results, 5 pulsotypes (80% of isolates) of Salmonella Typhimurium and 4 pulsotypes (64% of …