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Full-Text Articles in Life Sciences

Multi-Level Movement Response Of Invasive Wild Pigs (Sus Scrofa) To Removal, Guillaume Bastille-Rousseau, Peter E. Schlichting, David A. Keiter, Joshua B. Smith, John C. Kilgo, George Wittemyer, Kurt C. Vercauteren, James C. Beasley, Kim M. Pepin Jan 2021

Multi-Level Movement Response Of Invasive Wild Pigs (Sus Scrofa) To Removal, Guillaume Bastille-Rousseau, Peter E. Schlichting, David A. Keiter, Joshua B. Smith, John C. Kilgo, George Wittemyer, Kurt C. Vercauteren, James C. Beasley, Kim M. Pepin

USDA Wildlife Services: Staff Publications

BACKGROUND: Lethal removal of invasive species, such as wild pigs (Sus scrofa), is often the most efficient approach for reducing their negative impacts. Wild pigs are one of the most widespread and destructive invasive mammals in the USA. Lethal management techniques are a key approach for wild pigs and can alter wild pig spatial behavior, but it is unclear how wild pigs respond to the most common removal technique, trapping.We investigated the spatial behavior of wild pigs following intensive removal of conspecifics via trapping at three sites within the Savannah River Site, SC, USA. We evaluated changes in …


A Framework For Surveillance Of Emerging Pathogens At The Human-Animal Interface: Pigs And Coronaviruses As A Case Study, Kim M. Pepin, Ryan S. Miller, Mark Q. Wilber Jan 2021

A Framework For Surveillance Of Emerging Pathogens At The Human-Animal Interface: Pigs And Coronaviruses As A Case Study, Kim M. Pepin, Ryan S. Miller, Mark Q. Wilber

USDA Wildlife Services: Staff Publications

Pigs (Sus scrofa) may be important surveillance targets for risk assessment and risk-based control planning against emerging zoonoses. Pigs have high contact rates with humans and other animals, transmit similar pathogens as humans including CoVs, and serve as reservoirs and intermediate hosts for notable human pandemics. Wild and domestic pigs both interface with humans and each other but have unique ecologies that demand different surveillance strategies. Three fundamental questions shape any surveillance program: where, when, and how can surveillance be conducted to optimize the surveillance objective? Using theory of mechanisms of zoonotic spillover and data on risk factors, …


Prevalence And Distribution Of African Swine Fever Virus In Swine Feed After Mixing And Feed Batch Sequencing, C. Grace Elijah, Jessie D. Trujillo, Cassandra K. Jones, Taeyong Kwon, Charles R. Stark, Konner R. Cool, Chad B. Paulk, Natasha N. Gaudreault, Jason C. Woodworth, Igor Morozov, Carmina Gallardo, Jordan T. Gebhardt, Jurgen A. Richt Jan 2021

Prevalence And Distribution Of African Swine Fever Virus In Swine Feed After Mixing And Feed Batch Sequencing, C. Grace Elijah, Jessie D. Trujillo, Cassandra K. Jones, Taeyong Kwon, Charles R. Stark, Konner R. Cool, Chad B. Paulk, Natasha N. Gaudreault, Jason C. Woodworth, Igor Morozov, Carmina Gallardo, Jordan T. Gebhardt, Jurgen A. Richt

Kansas Agricultural Experiment Station Research Reports

As the United States maintains trade with countries where African swine fever virus (ASFV) is endemic, it is critical to have methods that can detect and mitigate the risk of ASFV in potentially contaminated feed or ingredients. Therefore, the objectives of this study were to 1) evaluate feed batch sequencing as a mitigation technique for ASFV contamination in a feed mill, and 2) determine if a feed sampling method could identify ASFV following experimental inoculation. Batches of feed were manufactured in a BSL-3Ag room at Kansas State University’s Biosafety Research Institute in Manhattan, KS. First, the pilot feed manufacturing system …


Prevalence And Distribution Of African Swine Fever Virus In Swine Feed After Mixing And Feed Batch Sequencing, C. Grace Elijah, Jessie D. Trujillo, Cassandra K. Jones, Taeyong Kwon, Charles R. Stark, Konner R. Cool, Chad B. Paulk, Natasha N. Gaudreault, Jason C. Woodworth, Igor Morozov, Carmen Gallardo, Jordan T. Gebhardt, Jurgen A. Richt Jan 2021

Prevalence And Distribution Of African Swine Fever Virus In Swine Feed After Mixing And Feed Batch Sequencing, C. Grace Elijah, Jessie D. Trujillo, Cassandra K. Jones, Taeyong Kwon, Charles R. Stark, Konner R. Cool, Chad B. Paulk, Natasha N. Gaudreault, Jason C. Woodworth, Igor Morozov, Carmen Gallardo, Jordan T. Gebhardt, Jurgen A. Richt

Kansas Agricultural Experiment Station Research Reports

As the United States maintains trade with countries where African swine fever virus (ASFV) is endemic, it is critical to have methods that can detect and mitigate the risk of ASFV in potentially contaminated feed or ingredients. Therefore, the objectives of this study were to 1) evaluate feed batch sequencing as a mitigation technique for ASFV contamination in a feed mill, and 2) determine if a feed sampling method could identify ASFV following experimental inoculation. Batches of feed were manufactured in a BSL-3Ag room at Kansas State University’s Biosafety Research Institute in Manhattan, KS. First, the pilot feed manufacturing system …


Performance Differences Between Two Groups Of Commercial Pigs Following Experimental Infection With Prrs Virus 1-7-4, Erin Little Jan 2019

Performance Differences Between Two Groups Of Commercial Pigs Following Experimental Infection With Prrs Virus 1-7-4, Erin Little

Electronic Theses and Dissertations

Porcine reproductive and respiratory syndrome virus (PRRSV) is the costliest swine disease in North America. Vaccines and management alone have not been effective in controlling this disease. Genetic selection for resilience may be a complimentary approach for controlling PRRSV. The objective of this study was to estimate performance differences between two groups of pigs from the same commercial line following infection with PRRSV 1-7-4: 1) pigs sired by boars selected based on a standard index (TN-S), which emphasized feed efficiency and carcass quality; and 2) pigs sired by boars selected based on an experimental index (TN-E), which emphasized feed intake, …


Effects Of Medium Chain Fatty Acid Application In Swine Feed On Porcine Epidemic Diarrhea Virus, A. B. Lerner, R. A. Cochrane, J. T. Gebhardt, S. S. Dritz, C. K. Jones, J. M. Derouchey, M. D. Tokach, R. D. Goodband, J. Bai, E. Porter, J. Anderson, P. C. Gauger, D. R. Magstadt, J. Zhang, B. Bass, T. P. Karnezos, B. De Rodas, J. C. Woodworth Jan 2019

Effects Of Medium Chain Fatty Acid Application In Swine Feed On Porcine Epidemic Diarrhea Virus, A. B. Lerner, R. A. Cochrane, J. T. Gebhardt, S. S. Dritz, C. K. Jones, J. M. Derouchey, M. D. Tokach, R. D. Goodband, J. Bai, E. Porter, J. Anderson, P. C. Gauger, D. R. Magstadt, J. Zhang, B. Bass, T. P. Karnezos, B. De Rodas, J. C. Woodworth

Kansas Agricultural Experiment Station Research Reports

Medium chain fatty acid (MCFA) application has been identified as a promising strategy to decrease viral pathogen transmission in swine feed. Four experiments were conducted to: 1) determine if MCFAs are effective when applied to feed both prior to and after porcine epidemic diarrhea virus (PEDV) inoculation measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR), 2) evaluate the effects of varying amounts and combinations of MCFA measured by qRT-PCR, and 3) evaluate selected MCFA treatments in a bioassay. In Exp. 1, treatments were arranged in a 2 × 2 + 1 factorial with the main effects of chemical treatment …


Anticoagulant Rodenticide Residues In Game Animals In California, Stella C. Mcmillin, Robert H. Poppenga, Shannon C. Chandler, Deana L. Clifford Jan 2018

Anticoagulant Rodenticide Residues In Game Animals In California, Stella C. Mcmillin, Robert H. Poppenga, Shannon C. Chandler, Deana L. Clifford

USDA Wildlife Services: Staff Publications

Anticoagulant rodenticides (ARs) are used to control rodents around homes, buildings, and in agriculture. They have been found widely in predatory and scavenging wildlife as a result of secondary exposure and less commonly in herbivores and omnivores from primary exposure. While predators and scavengers have been monitored for AR exposure, very little information is available about AR residues in edible muscle tissue of game animals. Game animals may be exposed to ARs through direct consumption of bait, ingestion of contaminated food or vegetation, or consumption of contaminated prey items. Carcasses of three species of game animals (black bear, wild pigs, …


Identifying Immuno-Dominant And Neutralizing Epitopes From K88 Fimbriae Of Enterotoxigenic Escherichia Coli (Etec), T. Lu, W. Zhang Jan 2017

Identifying Immuno-Dominant And Neutralizing Epitopes From K88 Fimbriae Of Enterotoxigenic Escherichia Coli (Etec), T. Lu, W. Zhang

Kansas Agricultural Experiment Station Research Reports

Enterotoxigenic Escherichia coli (ETEC) bacteria are the primary cause of diarrheal disease, especially porcine post-weaning diarrhea (PWD). Post-weaning diarrhea is one of the most common diseases in piglets 3 to 10 days after weaning and causes the loss of millions of dollars annually to United States swine industry and other countries. These ETEC bacteria produce two types of virulence factors: 1) fimbriae adhesins, which promote bacterial attachment and colonization in pig small intestine; and 2) enterotoxins that disrupt fluid homeostasis and cause fluid hype-secretion and watery diarrhea. The F4 (K88) is the most important fimbria in ETEC bacteria causing PWD. …


Assessing The Effects Of Medium Chain Fatty Acids And Fat Sources On Porcine Epidemic Diarrhea Virus Viral Rna Stability And Infectivity, R. A. Cochrane, S. S. Dritz, J. C. Woodworth, A. R. Huss, C. R. Stark, M. Saensukjaroenphon, J. M. Derouchey, M. D. Tokach, R. D. Goodband, J. Bai, Qi Chen, Jianqiang Zhang, Phillip Charles Gauger, Rachel J. Derscheid, Rodger G. Main, C. K. Jones Jan 2016

Assessing The Effects Of Medium Chain Fatty Acids And Fat Sources On Porcine Epidemic Diarrhea Virus Viral Rna Stability And Infectivity, R. A. Cochrane, S. S. Dritz, J. C. Woodworth, A. R. Huss, C. R. Stark, M. Saensukjaroenphon, J. M. Derouchey, M. D. Tokach, R. D. Goodband, J. Bai, Qi Chen, Jianqiang Zhang, Phillip Charles Gauger, Rachel J. Derscheid, Rodger G. Main, C. K. Jones

Kansas Agricultural Experiment Station Research Reports

Research has confirmed that chemical treatments, such as medium chain fatty acids (MCFA) and commercial formaldehyde, can be effective to reduce the risk of porcine epidemic diarrhea virus (PEDV) cross-contamination in feed. However, the efficacy of individual MCFA levels are unknown. The objective of this study is to compare the efficacy of commercially-available sources of MCFA and other fat sources versus a synthetic custom blend of MCFA to minimize the risk of PEDV cross-contamination as measured by qRT-PCR and bioassay. Treatments were arranged in a 17 × 4 plus 1 factorial with 17 chemical treatments: 1) Positive control with PEDV …


Evaluating The Inclusion Level Of Medium Chain Fatty Acids To Reduce The Risk Of Porcine Epidemic Diarrhea Virus In Complete Feed And Spray-Dried Animal Plasma, R. A. Cochrane, S. S. Dritz, J. C. Woodworth, A. R. Huss, C. R. Stark, M. Saensukjaroenphon, J. M. Derouchey, M. D. Tokach, R. D. Goodband, J. Bai, Qi Chen, Jianqiang Zhang, Phillip Charles Gauger, Rachel J. Derscheid, Drew Robert Magstadt, Paulo Elias Arruda, Alejandro Ramirez, Rodger G. Main, C. K. Jones Jan 2016

Evaluating The Inclusion Level Of Medium Chain Fatty Acids To Reduce The Risk Of Porcine Epidemic Diarrhea Virus In Complete Feed And Spray-Dried Animal Plasma, R. A. Cochrane, S. S. Dritz, J. C. Woodworth, A. R. Huss, C. R. Stark, M. Saensukjaroenphon, J. M. Derouchey, M. D. Tokach, R. D. Goodband, J. Bai, Qi Chen, Jianqiang Zhang, Phillip Charles Gauger, Rachel J. Derscheid, Drew Robert Magstadt, Paulo Elias Arruda, Alejandro Ramirez, Rodger G. Main, C. K. Jones

Kansas Agricultural Experiment Station Research Reports

Research has confirmed that chemical treatments, such as medium chain fatty acids (MCFA) and commercial formaldehyde, can be effective to reduce the risk of porcine epidemic diarrhea virus (PEDV) cross-contamination in feed. However, the efficacy of MCFA levels below 2% inclusion is unknown. The objective of this experiment was to evaluate if a 1% inclusion of MCFA is as effective at PEDV mitigation as a 2% inclusion or formaldehyde in swine feed and spray-dried animal plasma (SDAP). Treatments were arranged in a 4 × 2 × 7 plus 2 factorial with 4 chemical treatments: 1) PEDV positive with no chemical …


Evaluating The Impact Of Vevovitall And/Or Crina As Potential Porcine Epidemic Diarrhea Virus Mitigation Strategies As Determined By Polymerase Chain Reaction Analysis And Bioassay, J. T. Gebhardt, J. C. Woodworth, C. K. Jones, M. D. Tokach, J. M. Derouchey, R. D. Goodband, R. A. Cochrane, C. R. Stark, J. Bergstrom, Phillip Charles Gauger, J. Bai, Qi Chen, Jianqiang Zhang, Rodger G. Main, S. S. Dritz Jan 2016

Evaluating The Impact Of Vevovitall And/Or Crina As Potential Porcine Epidemic Diarrhea Virus Mitigation Strategies As Determined By Polymerase Chain Reaction Analysis And Bioassay, J. T. Gebhardt, J. C. Woodworth, C. K. Jones, M. D. Tokach, J. M. Derouchey, R. D. Goodband, R. A. Cochrane, C. R. Stark, J. Bergstrom, Phillip Charles Gauger, J. Bai, Qi Chen, Jianqiang Zhang, Rodger G. Main, S. S. Dritz

Kansas Agricultural Experiment Station Research Reports

Feed and feed ingredients have been shown to be potential vectors of porcine epidemic diarrhea virus (PEDV). Potential strategies to mitigate the risk of disease transmission via feed and feed ingredients would be valuable to the swine and feed milling industries. Therefore, the objective of this experiment was to determine the impact of VevoVitall (5,000 ppm; DSM Nutritional Products Inc., Parsipanny, NJ), CRINA (200 ppm; DSM Nutritional Products Inc., Parsipanny, NJ), and a combination of both products (COMBINATION; 5,000 ppm VevoVitall and 200 ppm CRINA) as feed additives with potential to mitigate the risk of PEDV, in swine gestation diet …


Evaluation Of The Effects Of Flushing Feed Manufacturing Equipment With Chemically- Treated Rice Hulls On Porcine Epidemic Diarrhea Virus Cross Contamination During Feed Manufacturing, J. T. Gebhardt, J. C. Woodworth, C. K. Jones, Phillip Charles Gauger, M. D. Tokach, J. M. Derouchey, R. D. Goodband, M. Muckey, R. A. Cochrane, M. Niederwerder, C. R. Stark, J. Bai, Qi Chen, Jianqiang Zhang, Alejandro Ramirez, Rachel J. Derscheid, Rodger G. Main, S. S. Dritz Jan 2016

Evaluation Of The Effects Of Flushing Feed Manufacturing Equipment With Chemically- Treated Rice Hulls On Porcine Epidemic Diarrhea Virus Cross Contamination During Feed Manufacturing, J. T. Gebhardt, J. C. Woodworth, C. K. Jones, Phillip Charles Gauger, M. D. Tokach, J. M. Derouchey, R. D. Goodband, M. Muckey, R. A. Cochrane, M. Niederwerder, C. R. Stark, J. Bai, Qi Chen, Jianqiang Zhang, Alejandro Ramirez, Rachel J. Derscheid, Rodger G. Main, S. S. Dritz

Kansas Agricultural Experiment Station Research Reports

Various strategies have been proposed to mitigate potential risk of porcine epidemic diarrhea virus (PEDV) transmission via feed and feed ingredients. Wet decontamination has been found to be the most effective decontamination of feed mill surfaces; however, this is not practical on a commercial feed production-scale. Another potential mitigation strategy, easier to implement, would be using chemically-treated rice hulls flushed through the feed manufacturing equipment. The objective of this experiment was to determine the impact of MCFA- or formaldehyde-treated rice hull flush batches as potential PEDV mitigation strategies during feed manufacturing. Feed without evidence of PEDV RNA contamination was inoculated …


Effect Of Thermal Mitigation On Porcine Epidemic Diarrhea Virus (Pedv)- Contaminated Feed, R. A. Cochrane, L. L. Schumacher, S. S. Dritz, J. C. Woodworth, A. R. Huss, C. R. Stark, J. M. Derouchey, M. D. Tokach, R. D. Goodband, J. Bai, Qi Chen, Jianqiang Zhang, Phillip Charles Gauger, Rodger G. Main, C. K. Jones Jan 2015

Effect Of Thermal Mitigation On Porcine Epidemic Diarrhea Virus (Pedv)- Contaminated Feed, R. A. Cochrane, L. L. Schumacher, S. S. Dritz, J. C. Woodworth, A. R. Huss, C. R. Stark, J. M. Derouchey, M. D. Tokach, R. D. Goodband, J. Bai, Qi Chen, Jianqiang Zhang, Phillip Charles Gauger, Rodger G. Main, C. K. Jones

Kansas Agricultural Experiment Station Research Reports

Porcine Epidemic Diarrhea Virus (PEDV) is primarily transmitted by fecal-oral contamination. However, epidemiological evidence has shown that swine feed and ingredients may serve as potential vectors of transmission. Since it is known that PEDV is a heat-sensitive virus, we hypothesized that a conditioner and pellet mill mimicking commercial thermal processing would mitigate PEDV infectivity. To test this hypothesis, two experiments were designed to determine if different pellet mill conditioner retention times or temperatures would impact PEDV infectivity determined by polymerase chain reaction (PCR) analysis and bioassay. For the first study, a 3×3×2 factorial was utilized, with three pelleting temperatures (155, …


Utilizing Feed Sequencing To Decrease The Risk Of Porcine Epidemic Diarrhea Virus (Pedv) Cross-Contamination During Feed Manufacturing, L. L. Schumacher, R. A. Cochrane, J. C. Woodworth, C. R. Stark, C. K. Jones, Rodger G. Main, Jianqiang Zhang, Phillip Charles Gauger, S. S. Dritz, M. D. Tokach Jan 2015

Utilizing Feed Sequencing To Decrease The Risk Of Porcine Epidemic Diarrhea Virus (Pedv) Cross-Contamination During Feed Manufacturing, L. L. Schumacher, R. A. Cochrane, J. C. Woodworth, C. R. Stark, C. K. Jones, Rodger G. Main, Jianqiang Zhang, Phillip Charles Gauger, S. S. Dritz, M. D. Tokach

Kansas Agricultural Experiment Station Research Reports

Understanding key points of potential cross-contamination during the feed manufacturing process is important to developing efficacious methods to control or prevent transmission of pathogens into swine diets. In this study, an experiment was conducted involving 30 crossbred 10-d-old pigs that were used as a bioassay model for Porcine Epidemic Diarrhea Virus (PEDV) to determine the effects of feed batch sequencing on PEDV cross-contamination and subsequent infectivity. PEDV with a PCR cycle threshold value (Ct) of 11 was uniformly mixed into 4.5 kg of swine diet using a stainless steel bench top mixer validated for mixing efficiency. The inoculated feed was …


Evaluating Chemical Mitigation Of Porcine Epidemic Diarrhea Virus (Pedv) In Swine Feed And Ingredients, R. A. Cochrane, S. S. Dritz, J. C. Woodworth, A. R. Huss, C. R. Stark, R. A. Hesse, Jianqiang Zhang, M. D. Tokach, J. Bai, C. K. Jones Jan 2015

Evaluating Chemical Mitigation Of Porcine Epidemic Diarrhea Virus (Pedv) In Swine Feed And Ingredients, R. A. Cochrane, S. S. Dritz, J. C. Woodworth, A. R. Huss, C. R. Stark, R. A. Hesse, Jianqiang Zhang, M. D. Tokach, J. Bai, C. K. Jones

Kansas Agricultural Experiment Station Research Reports

Porcine Epidemic Diarrhea Virus (PEDV) is primarily transmitted by fecal-oral contamination. Research has confirmed swine feed or ingredients as potential vectors of transmission, so strategies are needed to mitigate PEDV in feed. The objective of this experiment was to evaluate the effectiveness of various chemical additives to prevent or mitigate post-processing PEDV contamination in swine feed and ingredients. Treatments were arranged in a 7 × 4 factorial with seven chemical treatments and four feed matrices. The chemical treatments included: negative control with no chemical addition, 0.3% commercial formaldehyde product, 1% sodium bisulfate, 1% sodium chlorate, 3% custom organic acid blend …


Ec75-219 Nebraska Swine Report, William Ahlschwede, T. E. Socha, Alfonso Torres-Medina, A. J. Lewis, P. J. Cunningham, Dwane R. Zimmerman, E. R. Peo Jr., Larry K. Mcmullen, Bobby D. Moser, D. L. Ferguson, Phillip H. Grabouski, Murray Danielson, Charles W. Francis, L. F. Elliott, J. A. Deshazer, Roger W. Mandigo, W. J. Goldner, R. D. Fritschen, Gary Zoubek Jan 1975

Ec75-219 Nebraska Swine Report, William Ahlschwede, T. E. Socha, Alfonso Torres-Medina, A. J. Lewis, P. J. Cunningham, Dwane R. Zimmerman, E. R. Peo Jr., Larry K. Mcmullen, Bobby D. Moser, D. L. Ferguson, Phillip H. Grabouski, Murray Danielson, Charles W. Francis, L. F. Elliott, J. A. Deshazer, Roger W. Mandigo, W. J. Goldner, R. D. Fritschen, Gary Zoubek

University of Nebraska-Lincoln Extension: Historical Materials

This 1975 Nebraska Swine Report was prepared by the staff in Animal Science and cooperating departments for use in the Extension and Teaching programs at the University of Nebraska-Lincoln. Authors from the following areas contributed to this publication: Swine Nutrition, swine diseases, pathology, economics, engineering, swine breeding, meats, agronomy, and diagnostic laboratory. It covers the following areas: breeding, disease control, feeding, nutrition, economics, housing and meats.


Pig Typhus (Salmonellosis Suis), L. Van Es Aug 1946

Pig Typhus (Salmonellosis Suis), L. Van Es

Historical Research Bulletins of the Nebraska Agricultural Experiment Station

Salmonellosis suis or pig typhus, also designated as pig typhoid, infectious necrotic enteritis, or "necro," is an infectious disease among young pigs. It constitutes a major factor in the mortality of juvenile swine, and as such must be regarded as a more or less perennial hazard in swine production. Infectious necrotic enteritis caused by Salmonella suipestifer is the subject of the present publication.


Swine Sanitation, L. Van Es Mar 1930

Swine Sanitation, L. Van Es

Nebraska Agricultural Experiment Station: Historical Circulars

From the very beginning of Nebraska's agricultural development its farmers have recognized that the production of swine must of necessity accompany the growing of corn. The latter, one of the state's most important staples, cannot be marketed in a more economical manner than after having been transformed into pork, bacon, and lard. As a result the state has for many years maintained a rather dense swine population mainly divided into large herds kept on relatively small areas of land. This density of population, as well as certain practices in management and selective breeding, has brought about conditions favorable for the …