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Fine Root Vitality And Its Application In A Minirhizotron Study Of A Co2 Enrichment In A Florida Scrub Oak Ecosystem, Brandon Todd Herbert
Fine Root Vitality And Its Application In A Minirhizotron Study Of A Co2 Enrichment In A Florida Scrub Oak Ecosystem, Brandon Todd Herbert
Biological Sciences Theses & Dissertations
Minirhizotron images are assigned color codes to approximate the age of individual roots in situ. Triphenyl-tetrazolium chloride (TTC) has been used to measure the vitality of plant tissue by detecting dehydrogenase activity. TTC was used to assess the vitality of root tissue by color in a scrub oak ecosystem at Kennedy Space Center in central Florida. Roots were excavated, sorted into four color classes and stained with TTC. The product of the reduction of TTC by dehydrogenase enzyme, formazan, was extracted and its absorbance recorded using a spectrophotometer. A significant difference (p2 enrichment being conducted on a scrub oak …