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Full-Text Articles in Life Sciences
In Ovo Evaluation Of Floramax-B11 On Marek's Disease Hvt Vaccine Protective Efficacy, Hatchability, Microbiota Composition, Morphometric Analysis, And Salmonella Enteritidis Infection In Broiler Chickens, Kyle Dean Teague
Graduate Theses and Dissertations
It has previously been shown that effective probiotics can accelerate gut maturation and the development of a normal microflora in poultry. This results in increased resistance to enteric pathogens encountered by chicks early in life. Our objective in experiments 1 and 2 was to evaluate the effect of in ovo administration of FloraMax®-B11 (FM) on Marek’s disease (MD) herpesvirus of turkeys (HVT) vaccine protective efficacy. In Exp. 1, day 18 White Leghorn embryos were randomly distributed in four groups, 1) HVT vaccinated, no MDV challenge, 2) HVT + FM vaccinated, no MDV challenge, 3) HVT vaccinated, challenge with virulent MDV, …
Phenotypic Analysis And Localization Of Leukocytes At The Site Of Intradermal Injection Of Phytohemagglutinin In Chickens, Kallie Alyce Sullivan
Phenotypic Analysis And Localization Of Leukocytes At The Site Of Intradermal Injection Of Phytohemagglutinin In Chickens, Kallie Alyce Sullivan
Graduate Theses and Dissertations
Phytohemagglutinin (PHA) is commonly used to gain insight into an individual’s cellular immuno-competence. PHA is typically injected intradermally (i.d.) into the skin (e.g. wing web) and the tissue swelling response is then monitored, whereby the extent of the swelling positively relates to the individual’s cellular immune system capabilities. Although i.d. injected PHA was shown to stimulate mononuclear cell- and basophil-infiltration to the site of injection, reports on temporal, qualitative and quantitative aspects of the local PHA response are limited. The objective of this study was to use the growing feather (GF) as a cutaneous test-site to assess and monitor the …
Innate Immunity In Chickens: In Vivo Responses To Different Pathogen Associated Molecular Patterns, Kristen Alicia Byrne
Innate Immunity In Chickens: In Vivo Responses To Different Pathogen Associated Molecular Patterns, Kristen Alicia Byrne
Graduate Theses and Dissertations
Pattern recognition receptors (PRRs) on host cells recognize motifs known as pathogen associated molecular patterns (PAMPs) that are common to groups of microbes. Examples include LPS on Gram-negative bacteria, the structural motif PGN common to all bacteria, MDP the smallest immunostimulatory unit of PGN, and poly I:C the dsRNA analog. PAMP recognition by and stimulation of the innate immune system is crucial to an individual’s ability to quickly limit microbial growth and stimulate the adaptive immune system. Characterization of the in vivo immune responses initiated by PAMPs has not been directly addressed. Using growing feathers (GF) as a novel intradermal …
Proteomic Characterization Of Eggshell Membranes And Their Effect On Poultry Physiology And Immunity, Sarbjeet Kaur Makkar
Proteomic Characterization Of Eggshell Membranes And Their Effect On Poultry Physiology And Immunity, Sarbjeet Kaur Makkar
Graduate Theses and Dissertations
The use of antibiotics in poultry growth and disease control has led to antibiotics resistant problem in human beings, which is a big concern among consumers. With the necessity for judicious use of antibiotics in poultry production, alternative strategies to improve disease resistance in poultry production are necessary. The research is more inclined towards using the natural products available to grow healthier and antibiotic free meat animals. In the context of exploring natural and sustainable resource of alternative to antibiotics, the biochemical milieu of eggshell membranes (ESM) were analyzed by using mass spectrometry techniques including matrix assisted laser desorption ionization …
Genetic Analysis Of Chromosomal Regions Affecting Sperm Mobility In The Chicken (Gallus Gallus Domesticus), Sharath Chandra Munnaluri
Genetic Analysis Of Chromosomal Regions Affecting Sperm Mobility In The Chicken (Gallus Gallus Domesticus), Sharath Chandra Munnaluri
Graduate Theses and Dissertations
This project is focused on determining the contributions of particular genetic loci to low sperm mobility in the chicken. Sperm mobility has been shown to be a major determinant of male fertility in broiler breeders. A whole genome SNP scan identified chromosomal regions (QTLs) that control sperm mobility. I used microsatellites from 4 chromosomal regions ( 15.151 on chromosome 6, 12.435 on chromosome Z, 12.341 on chromosome Z, and 17.214 on chromosome Z) to determine the association of these regions with sperm mobility in experimental lines divergently selected for mobility phenotype. Determination of the genetics underlying sperm mobility will identify …
Characterization Of The Vasotocin Receptor Subtype 4 (Vt4r) In The Brain And Pituitary Gland Of The Chicken, Gallus Gallus, Rajamani Selvam
Characterization Of The Vasotocin Receptor Subtype 4 (Vt4r) In The Brain And Pituitary Gland Of The Chicken, Gallus Gallus, Rajamani Selvam
Graduate Theses and Dissertations
The present study investigated the distribution of the vasotocin subtype four receptor (VT4R) in brain and pituitary gland of the chicken, Gallus gallus. The anterior pituitary cell types associated with the VT4R were also determined. Two polyclonal antibodies were raised in rabbit against a cocktail of peptides, 15 amino acids from the amino terminal region and 17 amino acids from the carboxy terminal region of VT4R receptor. The antibody was validated utilizing the Western blot and immunocytochemistry. A single band at 47KDa utilizing membrane protein extracts of chicken brain and pituitary tissues was shown for the VT4R antibody. A peptide …