Life Sciences Commons^™

Comparison Of The In Vitro Fermentation Characteristic Of Fractionated Alfalfa And Sainfoin, L R. Mcmahon, M Goto, T A. Mcallister, G A. Jones, K J. Cheng

IGC Proceedings (1997-2023)

Alfalfa and sainfoin leaves were lyophilized and ground (A, S, respectively) or were fractionated into soluble (A_SOL, S_SOL) and insoluble (A_INS, S_INS)components and lyophilized and incubated in vitro with diluted ruminal fluid for 24h. Whole leaf and soluble fractions were also incubated with polyethylene glycol 8000 (PEG). Gas production (GP), ammonia concentration [NH3] and volatile fatty acid (VFA) production were lower (P< 0.05) from S and SSOL than from A and ASOL Insoluble fractions differed only in [NH3] (SINS < AINS, P < 0.05). Inclusion of PEG increased (P < 0.05) GP, [NH3] and VFA production from whole leaf and soluble fractions of sainfoin, but not alfalfa. Inactivating the condensed tannins in sainfoin with PEG overcame differences in degradability between these two forages.

Effect Of Microbial Inoculant On Fermentation Period And Chemical Composition Of Sweet Sorghum Silage, Azizza Mala, Babo Fadlalla, Elnour Mohamed, Siran Wang, Junfeng Li, Tao Shao

IGC Proceedings (1997-2023)

Sorghum is one of the most suitable plants for silage production and becoming an increasingly important forage crop in many regions of the world. Due to its high water-soluble carbohydrates contents (WSC) and low buffer capacity, it is easy to ensile. This study evaluates the effect of adding Lactobacillus plantarum AZZ4 and Pediococcus acidilactici AZZ5 isolated from elephant grass on the fermentation period and chemical composition of sweet sorghum silage. Two strains of Lactobacillus plantarum subsp. Plantarum (AZZ4) , Pediococcus acidilactici (AZZ5), and one commercial bacteria Lactobacillus Plantarum, Ecosyl MTD/1(CB)) were chosen as additives at 6 log colony forming units …

Review: Potential Of Using Seaweed Silage Inoculated With Lactic Acid Bacteria And Its Impact Towards Sustainable Animal Feed, Azziza Mala, Subha Bhassu, Norhidayah Mohd Taufek, Najiah M. Sadali, Siran Wang, Shao Tao, Elnour Mohamed, Adibi N, Nor

IGC Proceedings (1997-2023)

Since ancient times, seaweed has been used to supplement animal feed in coastal areas.

In recent years, there has been a growing demand for seaweed as an animal feed. Effective preservation methods are required because of annual variations in seaweed availability and biochemical composition. Ensiling could be an effective method to preserve seaweeds for animal feed applications. Using lactic acid bacteria is a substitute biological technique for keeping and restoring the usual physiological state of the animal and increasing efficiency. The aim of this review is the potential of using lactic acid bacteria as an inoculant for seaweed silage for …

Making Round Bale Silage – Lessons Learned From An On-Farm Survey Of Baleage In Kentucky, Jimmy C. Henning, Jeff Lehmkuhler, Brandon Sears, Corinne Belton, L. Berg, Don Sorrell, April Wilhoit

IGC Proceedings (1997-2023)

The ability to make silage in round bale packages allows producers to avoid rain damage and produce high quality stored forage. The proliferation of bale wrappers (both individual and inline types) has made this technology more available to producers. Although the process for making baleage is well documented, producers have experienced poor fermentation and in some cases botulism toxicity from baleage. The parameters of good silage are well known and include pH below 5.0 and lactic acid concentrations above 3% on a DM basis. However, tests of farmer- produced baleage reveals that often these target values are not achieved. To …

Influence Of Grass Species And Sample Preparation On Ensiling Characteristics, D. J. R. Cherney, M. A. Alessi, J. H. Cherney

IGC Proceedings (1997-2023)

Laboratory silos are considered a practical method of comparing a number of treatments (O'Kiely, 1993). Cherney et al. (2004) reported that vacuum-sealed polyethylene bags effectively ensiled corn silage samples in the laboratory. Grasses, with their inherently higher buffering capacities and lower sugar levels, generally are more difficult to ensile. Objectives were to evaluate the influence of species and chopping (whole vs. shredded) on pH and volatile fatty acid profile of grasses ensiled in vacuum-sealed polyethylene bags and to assess the suitability of this method as a laboratory ensiling method.

The Effect Of Fermentation Of Calliandra Calothyrsus, Gliricidia Sepium, Leucaena Leucocephala And Maize Forage On Rumen Degradation And Microbial Protein Synthesis, H. Kato, F. B. Bareeba, Elly N. Sabiiti, C. Ebong

IGC Proceedings (1997-2023)

Ensiling as a means of storing green fodder by acidification has a profound effect on the chemical composition of the resultant silage. Ensiling could therefore, ameliorate the effects of anti-nutritional factors associated with browses. The objective of the experiment was to determine fermentation characteristics and rumen degradation (D) of ensiled Calliandra calothyrsus (C), Gliricidia sepium (G), Leucaena leucocephala (L) and maize forage.

Parameters Of Ensiled Maize With Biological And Chemical Additives, J. Grajewski, A. Potkański, K. Raczkowska-Werwińska, M. Twarużek, B. Miklaszewska

IGC Proceedings (1997-2023)

The amount of maize grown in Poland has increased rapidly. Nowadays it takes about 600,000 ha, 40% of which is used as silage. Changing climate in Poland, with dry summer followed by wet autumn with ground frost causes extensive moulds contamination and high presence of the fusarium toxins in the maize during the harvest. The norms accepted in the EU concerning the acceptable level of deoxynivalenol (DON) and zearalenol (ZON) in feedstuffs for cattle require detailed examination of this problem as it decides on the health quality and production results. The aim of the study was to evaluate the effects …

Field Beans And Spring Wheat As Whole Crop Silage: Yield, Chemical Composition And Fermentation Characteristics, L. Ericson, K. Arvidsson, K. Martinsson

IGC Proceedings (1997-2023)

There has been an increasing interest in field beans (Vicia faba L.) in recent years because of its N-fixating ability. The objective of this study was to compare the yield, chemical composition and fermentation characteristics of field bean/spring wheat as whole-crop silage ensiled with and without an additive.

The crop was drilled on 27 May 2003 at a seed rate of 205 kg/ha field beans and 68 kg/ha spring wheat. The crop was harvested at four different growth stages (Zadoks et al., 1974); end of blooming, (stage 69), when 50% of the pods had reached full length, …

Influence Of Different Alfalfa-Grass Mixtures And The Use Of Additives On Nutritive Value And Fermentation Of Silage, P. Lättemäe, U. Tamm

IGC Proceedings (1997-2023)

Legumes have a high nutritive value but they are known to be difficult to ensile and often result in poorly fermented silage. This is usually due to high buffering capacity and low available sugar concentration. However, the results have shown that silage quality can considerably be improved by using additives or when legume-grass mixtures are ensiled (Lättemäe & Tamm, 2002). Different legume-grass mixtures differ in their ensiling properties and also may affect the fermentation. The objective of this experiment was to study the effect of alfalfa-grass mixtures and the use of additives on nutritive value and fermentation of silage.

Effect Of Biological Additives In Red Clover – Timothy Conservation, A. Olt, H. Kaldmäe, E. Songisepp, O. Kärt

IGC Proceedings (1997-2023)

Red clover at early flower bud formation is difficult to ensile. For efficient improvement of the quality of leguminous silages, chemical additives are used. The present research focuses on the effectiveness of biological additives with different composition on the fermentation and quality of clover silage.

The Influence Of The Application Of A Biological Additive On The Fermentation Process Of Red Clover Silage, Ľ. Rajčáková, R. Mlynár, M. Gallo

IGC Proceedings (1997-2023)

In Slovakia, mainly in the submontane and mountainous regions, growing of red clover is an important source of proteinous feeds. It is grown on 3.0% of arable land. It was the aim of this work to verify the possibilities of using a biological additive in red clover silage conservation.

Profiling The Cell Wall Polysaccharides Of Hemp (Cannabis Sativa L.) Inflorescence And Hempseed And Exploring Their Application As Ruminant Feed Ingredients, Miranda Agbana

Theses and Dissertations--Animal and Food Sciences

Hemp (Cannabis sativa L.) is used in the United States as a source of CBD oil, fiber, and grain. While hempseeds are consumed whole or as dehulled hemp hearts, hempseed oil is also a desirable seed oil in human nutrition. The process of collecting hempseed oil leaves behind hempseed cakes that are abundant in protein and fiber. Feeding of hempseed cakes to animals, including ruminants, has only initially been explored. Extraction of CBD from hemp inflorescences generates organic waste that may find a second purpose as a fiber feed for cattle. Little is known about the exact fiber composition …

Production Of Distilled Spirits Using Grain Sorghum Through Liquid Fermentation, Thomas Weiss, Jikai Zhao, Ruijia Hu, Meicen Liu, Yonghui Li, Yi Zheng, Gordon Smith, Donghai Wang

School of Earth, Environmental, and Marine Sciences Faculty Publications and Presentations

The objectives of this research were to investigate the fermentation performance of US sorghum varieties for the production of distilled spirits as well as their associated coproducts and to study the formation of volatile compounds that are related to the flavor quality of the spirits. Three US sorghum varieties (red, white, and waxy sorghums) and four yeast strains (DADY, Ethanol Red, GR-2, and 71B) were used for distilled spirit production. Both sorghum variety and type of yeast strains had effects on alcohol concentration and alcohol yield. The alcohol concentration varied from 10.26 to 11.34% (v/v) while alcohol yield varied from …

Effects Of Pruning Timing, Leaf Removal, And Shoot Thinning On 'Midsouth' Winegrape Quality In South Mississippi, Haley Nicole Williams

Theses and Dissertations

‘MidSouth’, a relatively low maintenance interspecific hybrid bunch grape currently grown in South Mississippi, has low sugar and high acid levels for red wine use. Two studies, conducted at the Mississippi State University McNeill Research Unit in 2020 and 2021, determined the effects of pruning timing, leaf removal, and shoot thinning on ‘MidSouth’ development and fruit and wine quality. Treatments in the first study included early versus normal pruning timing, both with and without leaf removal, and treatments in the second study included leaf removal, shoot thinning, and control vines. Cluster temperatures, leaf chlorophyll, berries per cluster, berry and cluster …

The Effects Of Microbial Inoculation On Short-To-Long Fermentation And Aerobic Stability Of Grass-Legume Silage Ensiled In Big Bales, J. Jatkauskas, V. Vrotniakiene, K. L. Witt, G. Copani, I. Eisner

IGC Proceedings (1997-2023)

Wilted (35.9 % DM and 2.6% WSC) moderately difficult to ensile grass-legume mixture (red clover, alfalfa and timothy 50:20:30) was ensiled in cylindrical big bales (1.2 m hight and 1.2 m diameter) with a weight about 700 kg. The crop was ensiled for 8, 32 and 120 days with or without a microbial inoculation (SiloSolve^® FC containing Lactococcus lactis DSM 11037/1k2081 and Lactobacillus buchneri DSM 22501/1k20738 applied at 1.5×10⁵ CFU g^-1 forage). Ten bales per treatment were prepared for each fermentation period. After each period of ensiling the big bales were opened, sampled, and tested for fermentation …

Exploring Bioprocessing Technologies For Diverse Industrial Application Of Canola, Ahmad Fawzi N Alhomodi

Electronic Theses and Dissertations

Globally, canola is the second largest oilseed crop after soybean, which is processed for the commercial production of high value oil. The industrial processing steps for canola oil extraction include preprocessing (cleaning, pressing, flaking, and cooking), mechanical pressing, and/or organic solvents. This process results in large quantities of protein-rich (~ 40% dry basis) meal as a co-product. The meal is used partially in animal diet (<30% inclusion) due to high levels of antinutritional factors (ANFs) such as high fibers, phytic acid, and glucosinolates, and low metabolizable energy. Thus, this research was designed to examine various bioprocessing technologies (i.e., traditional sprouting, solid state fermentation, submerged state fermentation, co-culture fermentation, mild pretreatments, and their combinations) for possible advancement in canola use. Canola seed sprouting for 6-day period led to an increase in protein content and a reduction in ANFs and oil content of sprouts compared to ungerminated seed. Subsequent submerged state fermentation of 6- day old sprouts using three different strains (Aureobasidium pullulans, Trichoderma reesei and Neurospora crassa) further increased protein content and lowered ANFs. Solid-state fermentation of 144 h old sprouts using A. pullulans, N. crassa and T. reesei enhanced the protein content and reduced ANFs of sprouts. Sprouting canola seed for three days helped in hull removal, leading to high protein meal accompanied by low fiber and phytic acid level. Three-day seed sprouting had no effect on oil yield, but free fatty acid content was higher compared to seed oil. Co-culture fermentation of HECM under solid state process showed maximum reduction in fiber content with co-culture of A. pullulans and N. crassa while a combination of A. pullulans and T. reesei promoted the highest GLS and phytic acid reductions compared to other combinations, which indicated the advantage of coculture inoculation over monoculture in terms of ANFs reduction. Mild pretreatment of HECM using deionized water resulted in washed HECM (WHECM) with lower soluble sugars and GLS compared to untreated HECM, whereas protein and amino acid were concentrated due to the removal of soluble components. WHECM compared to HECM showed higher protein digestibility when fed to rainbow trout. Subsequent mono- and coculture fermentation of HECM and WHECM under submerged process resulted in higher protein and amino acid content and lower ANFs levels compared to uninoculated controls. The results of cellulase, endoglucanase and β-glucosidase activity indicated the crucial role of used substrates, fungi, fermentation modes (solid state/submerged stated) and inoculation methods (mono-/co-culture) on enzyme activities.

Survival Of Escherichia Coli 0157:H7 Added To Grass At Ensiling And Its Influence On Silage Fermentation, P. O’Kiely, C. Byrne, D. Bolton

IGC Proceedings (1997-2023)

Escherichia coli can cause health problems in humans and livestock. It is frequently found in faeces and soil, both of which can contaminate grass harvested for silage-making. To determine the survival of E. coli 0157:H7 during ensilage, and its influence on ensilage, unwilted precision-chop grass was ensiled in laboratory silos with the following additive treatments: (a) no additive, (b) E. coli 0157:H7 (Ec - non-infectious strain, at log₁₀ 4.5 colony forming units (cfu) g^-1), formic acid (FA), and (d) Ec + FA. Silos were stored at 15^oC. Three silos per treatment were opened on days …

Isolation And Identification Of Lactic Acid Bacteria Strains And Their Effects On The Fermentation Quality Of Elephant Grass (Cenchrus Purpureus) Silage, Azizza Mala, Babo Fadlalla, Zhihao Dong, Junfeng Li, Tao Shao

IGC Proceedings (1997-2023)

This study aims to isolate and identify lactic acid bacteria and examine their effects on the fermentation quality of elephant grass silage. The isolated strains were identified based on morphological, physiological and biochemical characteristics as well as 16S rRNA analysis. Three strains namely Pediococcus acidilactic (AZZ5), Lactobacillus plantarum subsp. Plantarum (AZZ4), Lactobacillus plantarum subsp. Argentoratensis (AZZ6) were isolated from elephant grass silage. Isolation of the microbes was done by serial dilution method. Three LAB and one commercial bacteria Lactobacillus Plantarum, Ecosyl MTD/1(CB)) were used as additives to fresh material of elephant grass. To follow the fermentation quality during ensiling, …

A Novel Viral Strategy For Host Factor Recruitment: The Co-Opted Proteasomal Rpn11 Protein Interaction Hub In Cooperation With Subverted Actin Filaments Are Targeted To Deliver Cytosolic Host Factors For Viral Replication, Melissa Molho, Wenwu Lin, Peter D. Nagy

Plant Pathology Faculty Publications

Positive-strand (+)RNA viruses take advantage of the host cells by subverting a long list of host protein factors and transport vesicles and cellular organelles to build membranous viral replication organelles (VROs) that support robust RNA replication. How RNA viruses accomplish major recruitment tasks of a large number of cellular proteins are intensively studied. In case of tomato bushy stunt virus (TBSV), a single viral replication protein, named p33, carries out most of the recruitment duties. Yet, it is currently unknown how the viral p33 replication protein, which is membrane associated, is capable of the rapid and efficient recruitment of numerous …

Sugar Cane Silage (Saccharum Officinarum L.) Hydrolyzed With Calcium Hydroxide, J. A. Lima, E. A. Cunha, E. F. Júnior, M. S. Bueno, F. Okamoto

IGC Proceedings (1997-2023)

No abstract provided.

The Effect Of Ensiling Rice Straw With Or Without Bacterial Inoculants On Fermentation Quality, Chemical Composition And Nutritive Value, Chuncheng Xu, Yimin Cai, Lujia Han, Xian Liu, Zengling Yang, Norio Yoshida

IGC Proceedings (1997-2023)

No abstract provided.

Effect Of Essential Oils Used As Inoculants On The Nutritive Value Of Barley Silage, A. V. Chaves, J. Baah, W. Z. Yang, T. A. Mcallister, C. Benchaar

IGC Proceedings (1997-2023)

No abstract provided.

Microbiological Profile Of Sugarcane Silages Inoculated With Lactobacillus Buchneri, José C. Pinto, Carla Luiza Da Silva Ávila, H. C. P. Figueiredo, A. R. Morais, Odilon G. Pereira, R. F. Schwan

IGC Proceedings (1997-2023)

No abstract provided.

Use Of By‐Products Derived From Neem Tree (Azadirachta Indica) In Livestock Production, W. Z. Yang, B. N. Ametaj

IGC Proceedings (1997-2023)

No abstract provided.

Enhancing The Production And Sustainability Of Pasture-Fed Beef Using Non-Traditional Legume Forages, Andrea I. Bolletta

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Despite the increasing worldwide demand for beef as a protein source, consumers are concerned about the sustainability of ruminant production systems. Their main concerns are animal welfare for feedlot-fed animals, greenhouse gas (GHG) emissions, global warming and worker safety. Traditional feedlot-based beef production systems have been associated with locally greater levels of soil, water and air contamination, as well as the overuse of antibiotics and growth hormones. The use of legume pastures such as cicer milkvetch (CMV) and birdsfoot trefoil (BFT), which fix their own nitrogen (N) and often contain beneficial secondary compounds such as tannins and provide for rapid …

Phylogenetic Diversity Of Arkansas Vineyard And Wine Microbiota, Natacha Cureau

Graduate Theses and Dissertations

Microorganisms inhabiting the soil, leaves, and grapes in vineyards influence the terroir, a set of environmental factors that impact wine characteristics. Previously, the main method to identify microorganisms was to culture on different media, but a large portion (> 99%) could not be cultured and were consequently unidentified. However, the emergence of new molecular tools has enabled further study and identification of microorganisms such as indigenous vineyard microbiota. The objective of this research was to investigate the phylogenetic diversity of Arkansas vineyard and wine microbiota using high-throughput sequencing. In terms of the Arkansas vineyards, the fungal and bacterial diversity of …

The Effect Of Different Types Of Inoculants On The Characteristics Of Alfalfa, Ryegrass And Red Clover-Ryegrass-Timothy Silage, Vilma Vrotniakiene, Jonas Jatkauskas

IGC Proceedings (1997-2023)

Silage inoculants based on lactic acid bacteria (LAB) have demonstrated improvements in energy and nutrient recovery from grass and legume silages by lowering the pH and shifting the fermentation towards lactic acid production, by reducing storage losses due to spoilage and by increasing the aerobic stability during feed-out. There are different mixtures of strains currently on the market.

The present study was conducted according to the Deutsche Landwirtschafts-Gesellschaft Guidelines for the testing of silage additives in order to assess which mixtures of lactic acid bacteria would have a greater potential to improve fermentation profile of alfalfa, perennial ryegrass and red …

Effects Of Different Feed Additives On Methane Emissions From Beef Cattle, P. Giraldo, A. Marín, L. A. Giraldo

IGC Proceedings (1997-2023)

The rate of accumulation of methane in the atmosphere from enteric fermentation in cattle has an important impact on the greenhouse effect and contributing to global warming. Additionally, methane emission reduces the energy efficiency of substrate fermention in the rumen. Under-standing the effect of the diet on enteric methane emissions could help to identify strategies to reduce emissions of this greenhouse gas. Therefore, the main objective of the present investigation was to determine the effect of nutritional additives such as monensin, fumaric acid, tannins of Acacia decurrens, and glycerol on methane production and other measures of fermentation characteristics using …

Fermentation Parameters Of Kikuyu Grass (Pennisetum Clandestinum) By In Vitro Gas Production Technique (Ivgpt), A. Marín, P. Giraldo, L. A. Giraldo

IGC Proceedings (1997-2023)

Methane (CH₄) is a byproduct of ruminal fermentation whose production is directly related to forage quality, which in turn is affected by a myriad of environmental factors. In general the quality of pastures in the tropics range from medium to poor due to a high content of lignocellulosic material of low digestibility and a low content of other components, such as soluble carbohydrates and protein (Correa et al, 2008). CH₄ produced by enteric fermentation from cattle rumen represents a major source of greenhouse gases (GHG). By measuring these emissions the impact of dairy production systems on …

Hedges And Woody Strips Browsing By Cattle On Pasture In Wallonia, Belgium, Sophie Vandermeulen, Mathilde Pascal, Christian Marche, Arnaud Pilet, Yves Beckers, Jérôme Bindelle

IGC Proceedings (1997-2023)

Shrub and tree forages are commonly used in animal production in many regions of the world. Nonetheless, in Western Europe, and especially in Wallonia in Belgium, hedges and woody strips have disappeared from the agricultural landscape over the past 60 years. Browse species are usually rich in plant secondary compounds such as tannins whose benefits on CH4 production and intestinal parasitism have been highlighted (Ramírez-Restrepo et al. 2010). Currently, agri-environmental measures taken by the Walloon government promote hedges and woody strips in pastures, raising the interest in browse species functionalities in ruminant production.

This study aimed at: (1) determining …