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Full-Text Articles in Life Sciences
Optimizing The Physical And Nutritional Environment Of Unleached Root-Zones, Curtis B. Adams
Optimizing The Physical And Nutritional Environment Of Unleached Root-Zones, Curtis B. Adams
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Unleached root-zones represent an environmental ideal by eliminating wasteful leaching of nutrients and water. NASA grows plants in space in unleached root-zones, incorporating polymer-coated fertilizer (PCF) into a ceramic media (Turface or Profile). However, lack of growth productivity in space has led to the need for research to improve the nutritional and physical environment of the root-zone, which is the objective of this research. PCF types are diverse in release characteristics and the effects of temperature and substrate water content have not been well characterized. In spite of widespread use, studies on chemical properties and applied studies to verify soil …
Low Co-Cultivation Temperature At 20oc Improved Agrobacterium Tumefaciens-Mediated Transformation Of Tobacco Leaf Disks, Guiying Su
LSU Master's Theses
I investigated the effect of co-cultivation temperature and binary Ti vectors on the fresh weight yield of leaf disks of tobacco (Nicotiana tabacum L. cv. Xanthi (nn, Smith) after Agrobacterium tumefaciens-mediated transformation. I concluded that the temperature at 20ºC during co-cultivation of leaf disks with A. tumefaciens was the most critical factor on the reproducible enhancement of fresh weight yield. The highest fresh weight increase and presumably transformation efficiency was achieved under the following experimental conditions: A. tumefaciens strain LBA4404 containing pLSU binary Ti vector was grown overnight at 28oC in liquid Agrobacterium medium and the concentration was adjusted to …