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Full-Text Articles in Life Sciences
Exploration Of The Gossypium Raimondii Genome Using Bionano Genomics Physical Mapping Technology, Christopher Jon Hanson
Exploration Of The Gossypium Raimondii Genome Using Bionano Genomics Physical Mapping Technology, Christopher Jon Hanson
Theses and Dissertations
Cotton is a crop with a large global economic impact as well as a large, complex genome. Most industrial cotton production is from two tetraploid species (Gossypium hirsutum L. and Gossypium barbadense L.) which contain two subgenomes, specifically the AT and DT subgenomes. The DT subgenome is nearly half the size of the AT subgenome in tetraploid cotton and is closely related to an extant D-genome Gossypium species, G. raimondii Ulbr. Characterization of the structural variants present in diploid D-genome should provide greater insight into the evolution of the DT subgenome in the tetraploid cotton. Bionano (BNG) optical mapping uses …
The Genome Sequence Of Gossypium Herbaceum (A1), A Domesticated Diploid Cotton, Alex J. Freeman
The Genome Sequence Of Gossypium Herbaceum (A1), A Domesticated Diploid Cotton, Alex J. Freeman
Theses and Dissertations
Gossypium herbaceum is a species of cotton native to Africa and Asia. As part of a larger effort to investigate structural variation in assorted diploid and polyploid cotton genomes we have sequenced and assembled the genome of G. herbaceum. Cultivated G. herbaceum is an A1-genome diploid from the Old World (Africa) with a genome size of approximately 1.7 Gb. Long range information is essential in constructing a high-quality assembly, especially when the genome is expected to be highly repetitive. Here we present a quality draft genome of G. herbaceum (cv. Wagad) using a multi-platform sequencing strategy (PacBio RS II, Dovetail …
Improving Cotton Agronomics With Diverse Genomic Technologies, Aaron Robert Sharp
Improving Cotton Agronomics With Diverse Genomic Technologies, Aaron Robert Sharp
Theses and Dissertations
Agronomic outcomes are the product of a plant's genotype and its environment. Genomic technologies allow farmers and researchers new avenues to explore the genetic component of agriculture. These technologies can also enhance understanding of environmental effects. With a growing world population, a wide variety of tools will be necessary to increase the agronomic productivity. Here I use massively parallel, deep sequencing of RNA (RNA-Seq) to measure changes in cotton gene expression levels in response to a change in the plant's surroundings caused by conservation tillage. Conservation tillage is an environmentally friendly, agricultural practice characterized by little or no inversion of …