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Suberin Biosynthesis And Deposition In The Wound-Healing Potato (Solanum Tuberosum L.) Tuber Model, Kathlyn Natalie Woolfson
Suberin Biosynthesis And Deposition In The Wound-Healing Potato (Solanum Tuberosum L.) Tuber Model, Kathlyn Natalie Woolfson
Electronic Thesis and Dissertation Repository
Suberin is a heteropolymer comprising a cell wall-bound poly(phenolic) domain (SPPD) covalently linked to a poly(aliphatic) domain (SPAD) that is deposited between the cell wall and plasma membrane. Potato tuber skin contains suberin to protect against water loss and microbial infection. Wounding triggers suberin biosynthesis in usually non-suberized tuber parenchyma, providing a model system to study suberin production. Spatial and temporal coordination of SPPD and SPAD-related metabolism are required for suberization, as the former is produced soon after wounding, and the latter is synthesized later into wound-healing. Many steps involved in suberin biosynthesis remain uncharacterized, and the mechanism(s) that regulate …
Evaluation Of The Water Stress-Inducible Promoter Wsi18 In The Model Monocot Brachypodium Distachyon, Patrick D. Langille
Evaluation Of The Water Stress-Inducible Promoter Wsi18 In The Model Monocot Brachypodium Distachyon, Patrick D. Langille
Electronic Thesis and Dissertation Repository
Water deficit-inducible promoters that function in multiple species are valuable components for engineering stress-tolerant crops. Wsi18 is a water deficit-inducible promoter native to Oryza sativa. In this study, Brachypodium distachyon (B. distachyon) was used to determine if Wsi18 retained its water deficit-inducible characteristics in another monocot. Transgenic B. distachyon plants, in which the Wsi18 promoter drove the expression of the uidA reporter gene, were developed and exposed to osmotic stress generated by mannitol, salt stress conditions, and the water deficit-signaling phytohormone abscisic acid (ABA). GUS histochemical assays demonstrated increased uidA expression in the leaves and stem of …