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Full-Text Articles in Life Sciences

Nitrogen Transfer From Cover Crops To The Subsequent Grain Crop And The Influence Of Variability In Winter Conditions, Spencer Heuchan Dec 2022

Nitrogen Transfer From Cover Crops To The Subsequent Grain Crop And The Influence Of Variability In Winter Conditions, Spencer Heuchan

Electronic Thesis and Dissertation Repository

Cover crops, which are used to provide ground cover after the harvest of the grain crop, can potentially improve the sustainability of agroecosystems by reducing nutrient losses. However, few data are available to document the extent to which cover crops improve both the retention of soil nitrogen (N) and the transfer of this N to the grain crop. The efficiency of this N transfer may be further influenced by variation in winter soil temperature; for example, reduced snow cover can increase the frequency and intensity of soil freezing, which can affect the survival of cover crops and the timing of …


Herbicide Mediated Selection Of Sulfonamide Resistant E. Coli, Laura P. Muntz Oct 2022

Herbicide Mediated Selection Of Sulfonamide Resistant E. Coli, Laura P. Muntz

Electronic Thesis and Dissertation Repository

Asulam is an herbicide that is structurally analogous to sulfonamide antibiotics (sulfas), with the potential to contribute to the global antibiotic resistance crisis by cross-selecting for sulfa-resistant bacteria. To determine if asulam can select for antibiotic resistant bacteria, isothermal titration calorimetry (ITC) was used to assess the binding affinity of asulam with the target protein of sulfas, dihydropteroate synthase (DHPS). ITC confirmed asulam interacts with DHPS, and in vitro directed evolution experiments showed that prolonged asulam exposure can select for sulfa-insensitive DHPS in E. coli. Since mobile sul genes also encode for sulfa-insensitive DHPS, the potential effect of asulam on …


Cadmium Uptake In Plants As Influenced By Selenium Uptake And Sulphate Availability, Marnie J. Demand Sep 2022

Cadmium Uptake In Plants As Influenced By Selenium Uptake And Sulphate Availability, Marnie J. Demand

Electronic Thesis and Dissertation Repository

With cadmium uptake by plants posing a risk to plants and consumers alike, strategies to reduce metal uptake are desirable. One strategy may be to apply selenium (as selenate) to the growth medium. I hypothesized selenate would yield greater lignification, with a higher proportion of cadmium bound to root cell walls. Consequently, higher selenium in plants would result in greater tolerance to cadmium. Additionally, since selenate is taken up in place of sulphate, providing the plants with high sulphate would inhibit uptake and translocation of selenium, mitigating selenate’s benefits of reducing cadmium uptake and translocation. Experimental results did not support …


The Biosynthesis Of Non-Protein Sulfur Amino Acid In Developing Seeds Of Common Bean, Zixuan Lu Sep 2022

The Biosynthesis Of Non-Protein Sulfur Amino Acid In Developing Seeds Of Common Bean, Zixuan Lu

Electronic Thesis and Dissertation Repository

The protein quality of common bean (Phaseolus vulgaris) is associated with the level of dietary essential sulphur amino acids – methionine and cysteine. Extra sulphur that cannot be stored in the protein pool accumulates as the non-protein amino acid S-methylcysteine (S-methylCys) and its dipeptide γ-glutamyl-S-methylcysteine (γ-Glu-S-methylCys). Previous studies have indicated that S-methylhomoglutathione (S-methylhGSH) is present in the developing seed of common bean. It is hypothesized that S-methylhGSH is the key intermediate in the biosynthetic pathway of the γ-Glu-S-methylCys that leads to …


Manipulating The Root Mycobiome To Improve Plant Performance And Reduce Pathogen Pressure In Corn (Zea Mays), Noor F. Saeed Cheema Jun 2022

Manipulating The Root Mycobiome To Improve Plant Performance And Reduce Pathogen Pressure In Corn (Zea Mays), Noor F. Saeed Cheema

Electronic Thesis and Dissertation Repository

Crop yield often varies within a field of a single genetically uniform crop plant, with the causes presumed to be a mix of both biotic and abiotic factors. Manipulating crop root mycobiomes could potentially increase yield by reducing pathogen impacts and improving access to soil water and nutrients. This study aimed to identify different fungal inoculation treatments that could increase the growth of corn seedlings sown in low productivity soils to that in high productivity soils and shift the root mycobiome composition. Fungal inoculation treatments did not have significantly different root mycobiome composition than seedlings grown in low yield control …


Role Of Hxxxd-Motif Acyltransferases In Suberin Biosynthesis, Yudelkis Indira Queralta Castillo Jun 2022

Role Of Hxxxd-Motif Acyltransferases In Suberin Biosynthesis, Yudelkis Indira Queralta Castillo

Electronic Thesis and Dissertation Repository

Suberin is a complex polymer comprising an aliphatic polyester and an aromatic polymer, co-deposited with soluble waxes. The aliphatic polyester is composed of fatty acids, ω-hydroxy fatty acids, α,ω-dicarboxylic acids, fatty alcohols, glycerol, and ferulate. In potato, alkyl ferulates, are a major component of suberin-associated waxes. The lignin-like aromatic polymer is comprised of hydroxycinnamic acids and monolignols. Members of the HXXXD/BAHD acyltransferase family are required for the biosynthesis of the aliphatic suberin polyester and suberin-associated waxes, but their possible role in the biosynthesis of the phenolic domain of suberin remains unexplored. Here I studied the function of three BAHD candidate …