Improved Cultivation Systems For Isolation Of The Colorado Potato Beetle Spiroplasma., Meghnad Konai, K. J. Hackett, D. L. Williamson, J. J. Lipa, J. D. Pollack, G. E. Gasparich, E. A. Clark, D. C. Vacek, R. F. Whitcomb
Aug 1996
Improved Cultivation Systems For Isolation Of The Colorado Potato Beetle Spiroplasma., Meghnad Konai, K. J. Hackett, D. L. Williamson, J. J. Lipa, J. D. Pollack, G. E. Gasparich, E. A. Clark, D. C. Vacek, R. F. Whitcomb
Gail Gasparich
In North America, the Colorado potato beetle, Leptinotarsa decemlineata, is often infected with the host-specific, gut-inhabiting Colorado potato beetle spiroplasma (CPBS). CPBS is apparently a commensal, but it may be useful in biocontrol if it can be transformed to express an insect-lethal gene. Difficulty in cultivating the organism, however, has hindered the development of a suitable transformation system. In this study, we eliminated the need for coculturing CPBS with insect cells. CPBS was reliably isolated with the BBL Anaerobic GasPak Jar system (low redox, enhanced CO(inf2)), which was easier to use and less expensive than insect cell coculture methods. A …
Pollen Dispersal In Low-Density Populations Of Three Neotropical Tree Species, E. A. Stacy, J. L. Hamrick, J. D. Nason, S. P. Hubbell, R. B. Foster, R. Condit
Jul 1996
Pollen Dispersal In Low-Density Populations Of Three Neotropical Tree Species, E. A. Stacy, J. L. Hamrick, J. D. Nason, S. P. Hubbell, R. B. Foster, R. Condit
John Nason
Studies of mating patterns of tropical trees, typically involving common species, have revealed that most species are outcrossed and that, in some cases, a significant fraction of outcross pollen moves long distances. We evaluated mating systems and effective pollen dispersal for three hermaphroditic, insect-pollinated Neotropical tree species, Calophyllum longifolium, Spondias mombin, and Turpinia occidentalis, all of which occurred at low adult densities at the study site. Mating patterns were estimated for each maternal tree within 84-ha populations of C. longifolium and S. mombin in 1992 and 1993 and within a 50-ha population of T. occidentalis in 1993. Each population was …
Montandon Marsh: A Vegetation Description Of A Potentially Endangered Wetland, E. Hochman, W. Abrahamson, J. Clark
Dec 1995
Montandon Marsh: A Vegetation Description Of A Potentially Endangered Wetland, E. Hochman, W. Abrahamson, J. Clark
Warren G. Abrahamson, II
No abstract provided.
