Life Sciences Commons^™

Risk-Based Oversight Of Experiments In The Environment, Henry I. Miller, Robert H. Burris, Anne K. Vidaver, Nelson A. Wivel

Department of Plant Pathology: Faculty Publications

The new biology has come of age. Basic research in fields ranging from immunology to plant biology has been transformed so as to be almost unrecognizable to those whose biology education ended before 1970. The spillover into commercial development likewise has been remarkable. Hardly a week passes without news of some new advance in an area such as therapeutics, vaccines, or plants and animals for food, feed, or fiber. These uses of biotechnology in "contained" laboratories, pilot plants, greenhouses, and production facilities have engendered little controversy. The National Institutes of Health Guidelines for Research Involving Recombinant DNA have exempted from …

Pesticide Use On Crops In Nebraska - 1987, Maurice Baker, Nancy Peterson, Shripat T. Kamble

Historical Research Bulletins of the Nebraska Agricultural Experiment Station

This is the third and most comprehensive study of pesticide use on crops in Nebraska. The first was completed in 1978 and the second one in 1982. The first study indicated that approximately 25 million pounds (11.34 million kg) of active ingredients were used on the major crops in Nebraska. This increased to approximately 30.2 million pounds (13.7 million kg) in 1982. The USDA requires accurate information to meet their responsibilities. Thus, this survey was undertaken to determine: 1) the use of pesticides on crops, pasture and rangeland and 2) to identify pest management practices.

Towards An Improved Taxonomy Of Xanthomonas, L. Vauterin, J. Swings, K. Kersters, M. Gillis, T. W. Mew, M. N. Schroth, N. J. Palleroni, D. C. Hildebrand, D. E. Stead, E. L. Civerolo, A. C. Hayward, H. Maraîte, R. E. Stall, A. K. Vidaver, J. F. Bradbury

Department of Plant Pathology: Faculty Publications

Improvement of the taxonomy of the genus Xanthomonas and especially of Xanthomonas campestris, which is subdivided into more than 125 pathovars, is discussed. Recent contributions to the taxonomy of Xanthomonas are reviewed, and on the basis of these data and unpublished data from several laboratories, the usefulness of different phenotypic, chemotaxonomic, and genotypic techniques is discussed. The heterogeneity of several X. campestris pathovars has been demonstrated by sodium dodecyl sulfate electrophoresis of whole-cell proteins and fatty acid fingerprinting. The host selectivity of the pathovars is not correlated with their relationships as revealed by DNA-DNA hybridization experiments. In order to …

Capping Of Tobacco Mosaic Virus Rna: Analysis Of Viral-Coded Guanylyltransferase-Like Activity, David Dunigan, Milton Zaitlin

Department of Plant Pathology: Faculty Publications

The 5’ end of tobacco mosaic virus (TMV) genomic RNA is capped with 7- methylguanosine. A virus-coded polypeptide with guanylyltransferase activity has been investigated. This enzyme is responsible for forming the 5’→5’ linkage of guanosine 5’-monophosphate to the 5’- diphosphate of an acceptor RNA, thereby forming the cap. A critical step in the mechanism for cap formation in the eukaryotic nucleus is for guanylyltransferase to bind covalently to guanosine 5’- monophosphate with the hydrolysis of pyrophosphate when guanosine 5’- triphosphate is the substrate. The TMV 126-kilodalton protein, which is most probably a component of the TMV replicase, was found to …

Proceedings Of The 17th Annual Meeting, Southern Soybean Disease Workers (March 20-22, 1990, Biloxi, Mississippi): Soybean Disease Control At A Crossroad, William S. Gazaway, Donald E. Hershman, J. Allen Wrather, Gary W. Lawrence, Glenn G. Hammes

Southern Soybean Disease Workers: Conference Proceedings

Contents

Southern Soybean Disease Workers Officers 1989-1990

Southern Soybean Disease Workers Program Committee 1989-1990

Southern Soybean Disease Workers Committee Chairmen 1989-1990

General session

Presidential address. B Gazaway

Keynote address. K Smith

Invited speakers

Future Impacts of Biotechnology on Soybean Production and Uses. X Delannay

Diseases of Soybean Associated with International Seed Trade. B Moore

Impact of Regulatory Change and GLP's on New Fungicide Discovery and Development. G Hammes

Graduate student papers

Double-Stranded RNA and Virus-Like Particles From the Soybean Stem canker Pathogen, Diaporthe phaseolorum var. caulivora. Y Lee, JP Snow, GT Berggren, and RA Valverde

Development of Soybean Varieties …

Blueberry Research Progress Reports, H Y. Forsythe Jr, Judith A. Collins, John M. Smagula, Susan Erich, Delmont Emerson, David E. Yarborough, Warren Hedstrom, Mary J. Boutet, Rodney J. Bushway, Alfred A. Bushway, Paul R. Hepler, William A. Halteman, Tom Degomez, E A. Osgood, Luc Guimond, David Lambert

Wild Blueberry Research Reports

The 1989 edition of the Blueberry Research Progress Reports was prepared for the Maine Wild Blueberry Commission and the University of Maine Wild Blueberry Advisory Committee by researchers with the Maine Agricultural Experiment Station and Maine Cooperative Extension Service at the University of Maine, Orono. Projects in this report include:

1. Control of Secondary Blueberry Pests

2. Monitory Methods, Economic Injury Levels, and Action Thresholds of Secondary Blueberry Pests

3. Control of Blueberry Maggot

4. Phosphorus Dose/Response Curve

5. Nitrogen-Phosphorus Study

6. Multiple Cropping of Wild Stands

7. Changes in Sugars and Organic Acids of Blueberries During Development

8. Investigation …

Degradation Of Wheat Streak Mosaic Virus Capsid Protein During Leaf Senescence, Myron K. Brakke, Rose N. Skopp, L. C. Lane

Department of Plant Pathology: Faculty Publications

Wheat streak mosaic virus capsid protein degraded in vivo by proteolysis as leaves senesced. The capsid protein of virus purified from young systemically infected leaves had an apparent size or 45 kDa in 12% sodium dodecyl sulfate-polyacrylamide gels with minor amounts of 43, 42, 33, and 3E kDa proteins. The proportion of smaller proteins increased with the age of the leaf. In some virus preparations only 3 1 kDa capsid protein was detected. In vitro proteolysis of virions with 45 kDa protein produced virions with 31 kDa protein. Virions with 31 kDa capsid protein sedimented slightly more slowly than those …

Coping With Brown Spot And Root Rots Of Lupins, Mark Sweetingham

Journal of the Department of Agriculture, Western Australia, Series 4

There is increasing concern that brown spot and root diseases may limit the long term viability of lupin cultivation in close rotations in the agricultural areas of Western Australia. Research has found several useful strategies to reduce the impact of brown spot and Pleiochaeta root rot so that losses can be kept to a minimum. Rhizoctonia has proved more difficult to combat. Although less widespread than Pleiochaeta, it has caused severe losses and appears to be increasing in incidence, particularly in the northern wheatbelt. Research has vastly expanded our knowledge of the strains of Rhizoctonia present in our soils and …

Stratification Not Required For Tree-Of-Heaven Seed Germination, William R. Graves

William R Graves

Tree-of-heaven (Ailanthus altissima (Mill.) Swingle) seed germination and shoot dry weight were determined after stratifying samaras at 5 and 25 °C for up to 28 days. Seeds of samaras stratified at 5 °C for 0, 4, and 12 days showed 70, 77, and 96% germination, respectively, 7 days after sowing. Fourteen days after sowing, 90% of nonstratified seeds had germinated, but shoot weight of nonstratified seedlings was 16% less than that of seedlings from fruit stratified at 5 °C for 12 days. Germination 7 days after sowing was greater after stratification at 5 °C than at 25 °C, but the …

Dominance Relationships Of Cycloheximide-Resistant Mutants Of Schizophyllum Commune Fr., Marjorie Meier Eerdmans, Sally A. Amundson, Todd A. Reinhart, Keith K. Klein

Journal of the Minnesota Academy of Science

We have isolated several mutants of Schizophyllum commune Fr. able to grow on media containing cycloheximide in concentrations up to 30 mg/L. Genetic analyses of the resistant phenotypes show them to be due to the action of a single gene located on the first linkage group between the A-mating type factor and a gene for an adenine requirement (ade-ll). We have analyzed the growth and development of dikaryons made homoallelic for either the resistant or sensitive alleles as well as the heteroallelic dikaryon. These dikaryons showed different abilities to fruit when placed on media containing various concentrations of cycloheximide. Homoallelic …

Identification Of Single Meloidogyne Juveniles By Polymerase Chain Reaction Amplification Of Mitochondrial Dna, T. S. Harris, L. J. Sandall, Thomas O. Powers

Department of Plant Pathology: Faculty Publications

Polymerase chain reaction (PCR) was used to amplify a specific 1.8-kb sequence of mitochondrial DNA from single juveniles and eggs from 17 populations of Meloidogyne incognita, M. hapla, M. javanica, and M. arenaria. Approximately 2 μg amplified product were produced per reaction. Restriction digestion of the amplified product with HinfI permitted discrimination of clonal lineages of the four species. Meloidogyne javanica, however, could not be separated from M. hapla by the enzymes used in these experiments. Various amplification conditions and nematode lysis procedures were examined in order to optimize the speed and quality of …