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- Grapevine vein clearing virus (3)
- Virus (3)
- Epidemiology (2)
- Grapevine (2)
- Infectious clone (2)
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- Resistance (2)
- Agroinfiltration (1)
- Allorhizobium vitis (1)
- Ampelopsis cordata (1)
- And detection (1)
- Anthracnose (1)
- Badnavirus (1)
- Black walnut (1)
- Botrytis cinerea (1)
- Crown gall (1)
- Cultivar (1)
- Defense genes (1)
- Diagnostics (1)
- Downy mildew (1)
- Emergent (1)
- Endemic (1)
- GVCV (1)
- Gene discovery (1)
- Genomic feature (1)
- Genotyping-by-sequencing (1)
- Gnomonia leptosyla (1)
- Grape aphids (1)
- Grapevine aphids (1)
- Grapevine vein clearing virus (1)
- Haplotype (1)
Articles 1 - 9 of 9
Full-Text Articles in Life Sciences
Natural Transmission Of Grapevine Vein Clearing Virus, Matthew Manu
Natural Transmission Of Grapevine Vein Clearing Virus, Matthew Manu
MSU Graduate Theses
More than 60 million tons of grapes are produced annually in the world, making them one of the most widely grown fruit crops. Despite grapes’ economic and health benefits, biotic stressors, such as viruses, cause significant loss to the grape and wine industry. One such virus is grapevine vein clearing virus (GVCV) which seriously threatens grape cultivation in the Midwest region of the United States. This virus has caused the removal of seven commercial vineyards since its discovery in 2004. About 34% of Ampelopsis cordata wild vines are infected with GVCV and serve as a primary inoculum for the spread …
Distribution Patterns Of Allorhizobium Vitis In Missouri Vineyards And Non-Vineyard Soils, Jacquelyn M. Wray
Distribution Patterns Of Allorhizobium Vitis In Missouri Vineyards And Non-Vineyard Soils, Jacquelyn M. Wray
MSU Graduate Theses
Crown gall disease causes significant economic loss to the grape and wine industry. Preventive strategies are most effective for mitigating the loss of grapevines in vineyards, as there is no known cure for this disease. The bacterium Allorhizobium vitis carrying a tumor-inducing (Ti) plasmid is the causative agent. A. vitis bacteria live systemically in the grapevine before causing visible symptoms and can survive in residual plant tissues and soil for more than two years. Diagnostic methods have been developed to detect A. vitis bacteria in grapevines and soil. However, more reliable, specific, and high-throughput diagnostics are still needed for screening …
Testing The Impact Of A Silencing Suppressor On Infectivity Of Grapevine Vein Clearing Virus Infectious Clone In Nicotiana Benthamiana, Wen Zhao
MSU Graduate Theses
Grapevine vein clearing virus (GVCV), the first DNA virus of the Badnavirus genus discovered in grapevines, is closely associated with grapevine vein-clearing disease. Through earlier research, Koch's postulates were partially met: GVCV was in all diseased plants; GVCV was introduced into a healthy grapevine through grafting and by aphids and caused the disease. However, more shreds of evidence are required to fulfill the last postulate, the same virus must be reisolated from the inoculated diseased grapevine (1). A full-length infectious clone of GVCV was previously constructed to provide evidence; however, its infectivity was not consistent. Therefore, the goal …
Prevalence, Genetic Relationships, And Quantity Of Grapevine Vein Clearing Virus In Aphis Illinoisenesis, Adam Louis Uhls
Prevalence, Genetic Relationships, And Quantity Of Grapevine Vein Clearing Virus In Aphis Illinoisenesis, Adam Louis Uhls
MSU Graduate Theses
Grapevine vein clearing virus (GVCV) is an emerging pathogen causing severe damage to cultivated grapevines in the Midwest area of the United States. The prevalence of GVCV has been reported in native Vitis spp. and Ampelopsis cordata, a close relative of vitis in the Vitaceae family, which act as a reservoir for the virus. GVCV can be transmitted from wild A. cordata to Vitis spp. by Aphis illinoisensis (grape aphids) under greenhouse conditions, but the prevalence and transmission in native populations remains unknown. Knowing the prevalence and diversity of GVCV variants in natural grape aphid populations would help monitor …
Phenotypic Study Of Anthracnose Resistance In Black Walnut And Building A Mapping Population, Sadie D. Land
Phenotypic Study Of Anthracnose Resistance In Black Walnut And Building A Mapping Population, Sadie D. Land
MSU Graduate Theses
Black walnut anthracnose, caused by Gnomonia leptostyla, is the most widespread and destructive disease affecting black walnut trees (Juglans nigra). Breeding cultivars for a higher resistance to anthracnose is a natural and efficient strategy for improving the health and production quality of black walnut trees. The two goals of this study were to reveal that the ‘Sparrow’ cultivar of black walnut contains a significantly higher resistance to anthracnose than the ‘Football’ cultivar when separated from environmental factors, and to expand the ‘Football’ × ‘Sparrow’ F1 mapping population to evaluate how the trait of resistance is inherited in the progeny. A …
Sequence Analysis Of The Ren1 Genomic Region From The Grapevine ‘Kishmish Vatkana’, Dani Joseph
Sequence Analysis Of The Ren1 Genomic Region From The Grapevine ‘Kishmish Vatkana’, Dani Joseph
MSU Graduate Theses
The REN1 region of the grapevine ‘Kishmish Vatkana’ was mapped as the locus that confers resistance to the economically important disease, grape powdery mildew. The purpose of this work was to extend the nucleotide sequence information of this region. By sequencing a heretofore unknown bacterial artificial chromosome clone, the sequence information of this region was extended by 46,890 nucleotides. Sequencing was performed using the third-generation sequencing method, named Oxford Nanopore Technology (ONT). In order to improve the accuracy of the sequence data, a modified ONT library preparation method was developed. ONT sequencing of a library prepared with the modified protocol …
Mapping A New Disease Resistance Locus In An F1 Progeny Derived From Two Grape Wild Relatives, Gaurab Bhattarai
Mapping A New Disease Resistance Locus In An F1 Progeny Derived From Two Grape Wild Relatives, Gaurab Bhattarai
MSU Graduate Theses
Linkage maps and quantitative trait loci (QTL) analysis have become essential tools for the positional cloning of agronomically important genes and for marker-assisted breeding. In this study, two North American grape species, Vitis rupestris and Vitis riparia, and their 294 F1 progeny were used to construct parental linkage maps and to perform QTL analysis for downy mildew resistance. Single nucleotide polymorphism (SNP) discovery was accomplished using genotyping-by-sequencing (GBS) and resulted in 348,888 SNPs. Of these, 11,063 informative SNP markers (3.17% of the original SNP dataset) were derived after filtering for various quality parameters and missing data. A two-way …
Grapevine Vein Clearing Virus: Epidemiological Patterns And Construction Of A Clone, Cory Von Keith
Grapevine Vein Clearing Virus: Epidemiological Patterns And Construction Of A Clone, Cory Von Keith
MSU Graduate Theses
Grapevine vein clearing virus (GVCV) is a recently discovered virus belonging to the Badnavirus genus. Characteristic to its name, the virus is associated with a disease where symptoms manifest as pronounced vein-clearing, resulting in severe berry deformation and vine decline in susceptible grape varieties. Sustainable production of wine is dependent on healthy plants. The associated disease is mainly found in Midwest vineyards. Attempts were made in this thesis to provide evidence of causality of the virus to the associated disease and to infer the historical path and migration pattern of GVCV. Conclusions and discussions will provide grape producers with the …
Assessment Of Resistance To Botrytis Cinerea In Arabidopsis Expressing Grapevine Sts Genes And Analysis Of New Grapevine Vein Clearing Virus Isolates, Li Su
MSU Graduate Theses
Grapevine stilbene synthase gene (STS) family is unusually large with more than 32 members of full-length genes. Resveratrol and piceid are synthesized in VaSTS7-, VvSTS22-, VaSTS22-transgenic Arabidopsis. To study the function of these STS genes in the resistance against Botrytis cinerea in vivo, lesion sizes on these STS transgenic Arabidopsis were compared to that on non-transgenic control Col-0, at 96 hours post inoculation with B. cinerea spores at 0, 200, or 1,000 spores/μl. In each experiment there were three biological replicates. Three lesion sizes from each replicate were averaged. The experiment was repeated three …