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Full-Text Articles in Life Sciences
Development Of Highly Variable Microsatellite Markers For The Tetraploid Silene Stellata (Caryophyllaceae), Juannan Zhou, Michele R. Dudash, Charles B. Fenster, Elizabeth A. Zimmer
Development Of Highly Variable Microsatellite Markers For The Tetraploid Silene Stellata (Caryophyllaceae), Juannan Zhou, Michele R. Dudash, Charles B. Fenster, Elizabeth A. Zimmer
Natural Resource Management Faculty Publications
Premise of the study:We designed and tested microsatellite markers for the North American native species Silene stellata (Caryophyllaceae) to investigate its population genetic structure and identify selection on floral design through male reproductive success.
Methods and Results: A total of 153 candidate microsatellite loci were isolated based on next-generation sequencing. We identified 18 polymorphic microsatellite loci in three populations of S. stellata, with di- or trinucleotide repeats. Genotyping results showed the number of alleles per locus ranged from six to 45 and expected heterozygosity ranged from 0.511 to 0.951. Five of these loci were successfully amplified in S. …
Next Generation Mapping Of Enological Traits In An F2 Interspecific Grapevine Hybrid Family, Shanshan Yang, Jonathan Fresnedo-Ramírez, Qi Sun, David C. Manns, Gavin L. Sacks, Anna Katherine Mansfield, James L. Luby, Jason Londo, Bruce I. Reisch, Lance Cadle-Davidson, Anne Y. Fennell
Next Generation Mapping Of Enological Traits In An F2 Interspecific Grapevine Hybrid Family, Shanshan Yang, Jonathan Fresnedo-Ramírez, Qi Sun, David C. Manns, Gavin L. Sacks, Anna Katherine Mansfield, James L. Luby, Jason Londo, Bruce I. Reisch, Lance Cadle-Davidson, Anne Y. Fennell
Agronomy, Horticulture and Plant Science Faculty Publications
In winegrapes (Vitis spp.), fruit quality traits such as berry color, total soluble solids content (SS), malic acid content (MA), and yeast assimilable nitrogen (YAN) affect fermentation or wine quality, and are important traits in selecting new hybrid winegrape cultivars. Given the high genetic diversity and heterozygosity of Vitis species and their tendency to exhibit inbreeding depression, linkage map construction and quantitative trait locus (QTL) mapping has relied on F1 families with the use of simple sequence repeat (SSR) and other markers. This study presents the construction of a genetic map by single nucleotide polymorphisms identified through genotyping-by-sequencing …
Toward The Elucidation Of Cytoplasmic Diversity In North American Grape Breeding Programs, Jonathan Fresnedo-Ramirez, Qi Sun, Chin-Feng Hwang, Craig A. Ledbetter, David W. Ramming, Anne Fennell, M. Andrew Walker, James J. Luby, Matthew D. Clark, Jason P. Londo, Lance Cadle-Davidson, Gan-Yuan Zhong
Toward The Elucidation Of Cytoplasmic Diversity In North American Grape Breeding Programs, Jonathan Fresnedo-Ramirez, Qi Sun, Chin-Feng Hwang, Craig A. Ledbetter, David W. Ramming, Anne Fennell, M. Andrew Walker, James J. Luby, Matthew D. Clark, Jason P. Londo, Lance Cadle-Davidson, Gan-Yuan Zhong
Agronomy, Horticulture and Plant Science Faculty Publications
Plants have an intriguing tripartite genetic system: Nuclear genome 9 Mitochondria 9 Plastids and their interactions may impact germplasm breeding. In grapevine, the study of cytoplasmic genomes has been limited, and their role with respect to grapevine germplasm diversity has yet to be elucidated. In the present study, the results of an analysis of the cytoplasmic diversity among 6073 individuals (comprising cultivars, interspecific hybrids and segregating progenies) are presented. Genotyping by sequencing (GBS) was used to elucidate plastid and mitochondrial DNA sequences, and results were analyzed using multivariate techniques. Single nucleotide polymorphism (SNP) effects were annotated in reference to plastid …