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Tissue Culture In Rice (Oryza Sativa L. Subsp. Japonica Var. Lemont), Young M. Woo

Masters Theses

The purpose of this study was to obtain callus induction and subsequent plantlet regeneration from various explants of rice (Oryza sativa L. subsp. japonica var. Lemont). Callus induction occurred from the somatic cells of ovary wall, immature and mature embryos, immature endosperm, stem nodes, and seedlings. Plantlets were regenerated from embryo-, endosperm-, and stem node-derived calli on differentiation media. Stem nodes were the most responsive to callus induction. Requirements for optimal callus formation and plantlet regeneration differed from each culture. The study investigated the effects of cold treatment of explants, initial culture in darkness, media, and subculture for individual explant …