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Correlation Of Visualized Glucocorticoid Receptor And Apoptosis In Individual Clones Of Nb2 Cells, Devang M. Patel
Correlation Of Visualized Glucocorticoid Receptor And Apoptosis In Individual Clones Of Nb2 Cells, Devang M. Patel
Theses and Dissertations
The Nb2 lymphoma cell line is susceptible to glucocorticoid (GC)-induced apoptosis in the absence of mitogen and this effect is inhibited by the mitogen prolactin (PRL). This process is believed to be mediated by the glucocorticoid receptor (GR) and various downstream signals. The purpose of this study was to use the limiting dilution method to isolate subsets of Nb2 clones which expressed either highly enriched amounts of GR or an absence of GR and to relate the amount of GR to the magnitude of GC induced cytolysis. The presence of GR in these clones was determined by immunocytochemistry (ICC) using …
Examination Of Signals Involved In Dexamethasone Induced Apoptosis In Nb2 Lymphoma Cells, Suhas Badarinath
Examination Of Signals Involved In Dexamethasone Induced Apoptosis In Nb2 Lymphoma Cells, Suhas Badarinath
Theses and Dissertations
Consistent with previous studies, we demonstrated that dexamethasone (Dex) caused cytolysis/apoptosis in log phase Nb2 lymphoma cells, while prolactin (Prl) inhibited this effect. The Nb2 model was used to investigate the mechanisms of apoptosis control with the aid of immunocytochemistry (ICC). We established with absorption the specificity of staining due to Fas, Fas Ligand, Bcl-2, and Bax antibodies (the specificity of anti-p53 could not be verified). Dex-induced cytolysis/apoptosis was detected in synchronized (G⁰/G¹) cells after 6 and 8 hours of Dex exposure. A novel, computerized technique was used to quantitate the proportion of cells immunostained for the signals of interest …