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Longitudinal, Diet-Induced Weight Gain Is Associated With Increased Blood Monocytes And Reduced Tlr4 Expression, Kelley Strohacker, Richard J. Simpson, Katie C. Carpenter, Whitney L. Breslin, Brian K. Mcfarlin
Longitudinal, Diet-Induced Weight Gain Is Associated With Increased Blood Monocytes And Reduced Tlr4 Expression, Kelley Strohacker, Richard J. Simpson, Katie C. Carpenter, Whitney L. Breslin, Brian K. Mcfarlin
International Journal of Exercise Science
Excessive weight gain increases systemic inflammation resulting in increased disease risk. Toll-like receptor 4 (TLR4) reportedly mediates increases in inflammation; however, its role has not been fully evaluated. Objective. The purpose of this study was to determine the longitudinal effect of diet-induced weight gain on blood monocyte concentration and cell-surface TLR4 expression. Research Methods & Procedures. Male CD-1 mice were randomly assigned to high-fat (HF, n = 12) or low-fat (LF, n = 13) groups. Non-lethal, saphenous vein blood samples were collected at 0, 4, 8 and 12 weeks of treatment. Three-color flow cytometry was used to measure monocyte (CD11b+/CD14+) …