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Full-Text Articles in Life Sciences
Reactive Oxygen Species Damage And Consequences For Mitochondrial Function In The Hibernating Thirteen-Lined Ground Squirrel, Ictidomys Tridecemlineatus, Brynne Duffy
Electronic Thesis and Dissertation Repository
Hibernators experience changes in blood flow, similar to ischemia-reperfusion injury, which may lead to oxidative damage. I hypothesized that suppression of mitochondrial metabolism during hibernation protects against such damage. I compared oxidative damage and antioxidant capacity in tissues and isolated liver mitochondria among summer, torpid, and interbout euthermic thirteen-lined ground squirrels (TLGS). I found less oxidative tissue damage during summer than hibernation, but no mitochondrial differences. I also compared metabolic activity of isolated mitochondria before and after five minutes of anoxia, followed by reoxygenation, among hibernation states and rats. Anoxia-reoxygenation decreased state-three respiration (ST3) in all groups, with rat mitochondria …
The Effect Of Anoxia On Mitochondrial Function In A Hibernator (Ictidomys Tridecemlineatus), Leah Hayward
The Effect Of Anoxia On Mitochondrial Function In A Hibernator (Ictidomys Tridecemlineatus), Leah Hayward
Electronic Thesis and Dissertation Repository
Hibernation protects mammalian tissues against ischemia-reperfusion injury, but the underlying biochemical mechanisms are unknown. I hypothesized that the mechanisms allowing for mitochondrial metabolic flexibility during hibernation permit anoxia tolerance and contribute to tissue ischemia-reperfusion tolerance. I assessed mitochondrial performance before and after five minutes of anoxia in liver mitochondria isolated from thirteen-lined ground squirrels. I compared this anoxia effect among animals that were summer active (SA), or during hibernation (in torpor or interbout euthermia; IBE). Anoxia decreased state 3 respiration in all groups, but mitochondria isolated from torpid squirrels were least affected; these decreases paralleled decreased activity of electron transport …
Characterization Of Blood-Derived Human Progenitor Cells For Vascular Regeneration, David Putman
Characterization Of Blood-Derived Human Progenitor Cells For Vascular Regeneration, David Putman
Electronic Thesis and Dissertation Repository
Cardiovascular disease (CVD) remains a leading cause of premature death worldwide. Despite advances in treatment of ischemic diseases including myocardial infarction and peripheral arterial disease (PAD) there remains a need for effective revascularization therapies. Although early cell therapy trials investigated use of MNC from autologous bone marrow, emerging evidence indicates that purified progenitor cell populations are required to induce optimal vascular regeneration. In addition, autologous cells show reduced efficacy due to CVD-related progenitor dysfunction. This thesis presents preclinical studies characterizing several blood-derived cell types for the development of cellular therapies to treat PAD, focusing on the use of allogeneic umbilical …