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Use Of 52Cr For The Quantitative Determination Of Red Cell Volume, Claude Michael Hanbury
Use Of 52Cr For The Quantitative Determination Of Red Cell Volume, Claude Michael Hanbury
Chemistry & Biochemistry Theses & Dissertations
Chromium-52 has recently been suggested for use as a new agent to label red cells for the in vivo quantitation of red cell volume (1). In this paper, the development and validation of a routine 52cr labeling procedure is described.
The accuracy, precision, and detection limits of chromium analysis by Zeeman effect atomic absorption spectroscopy was evaluated in the concentration range of 1 - 10 ug Cr/L.
Red cell chromium uptake was evaluated as a function of time, temperature, and concentration. Red cells labeled with a 2.5 mg/L chromium solution for 30 minutes at room temperature exhibited optimal label …