Life Sciences Commons^™

Development Of A Biosensor For Investigating Membrane Curvature Sorting, Joshua C. Black

Electronic Theses and Dissertations

The physical structure of cellular membranes plays a critical role in lipid and protein sorting. A novel biosensor was developed to probe the influence of curvature on sorting. This biosensor mimics large, two-dimensional membranes in dynamic equilibrium, achieves high spatial resolution between curvature and molecules of interest, and has high sensitivity, enough for single particle detection. The biosensor consists of continuous supported lipid bilayer formed over nanoparticles (40 to 200 nm diameter) deposited on a glass substrate. The nanoparticles determine the extent of curvature. This biosensor is the first to observe large-scale 2-dimensional diffusion of biomolecules on a supported lipid …

A Glyptosaurine Lizard From The Eocene (Late Uintan) Of San Diego, California, And Implications For Glyptosaurine Evolution And Biogeography, David Moscato

Electronic Theses and Dissertations

Glyptosaurinae is an extinct subfamily of lizards of the family Anguidae. Glyptosaurine lizards are known exclusively from the Paleogene of North America and Eurasia, reaching their peak of diversity and distribution in the Eocene. In North America these lizards are largely restricted to the intermontane basins along the Rocky Mountain range, with only sparse, indeterminately-identified skeletal elements known from outside of this region. Glyptosaurine lizards are split into two tribes: the monophyletic Glyptosaurini and paraphyletic “Melanosaurini”. Within Glyptosaurini, the most common and widespread genus is Glyptosaurus. In this study I describe a new specimen assignable to G. sylvestris, …

Systematics, Climate, And Ecology Of Fossil And Extant Nyssa (Nyssaceae, Cornales) And Implications Of Nyssa Grayensis Sp. Nov. From The Gray Fossil Site, Northeast Tennessee, Nathan R. Noll

Electronic Theses and Dissertations

The Late Hemphillian (latest Miocene or earliest Pliocene, 7-4.5 Ma) Gray Fossil Site in northeastern Tennessee is interpreted to represent a lacustrine paleokarst fed by a river or stream. This research focuses on the morphological and systematic relations of Nyssa endocarps (fruit pits) from the fossil site to extinct and extant Nyssa species. A combination of metric and nonmetric traits allows recognition of a new species: Nyssa grayensis sp. nov. This fossil species shares the most similarities with the extant Nyssa ogeche Bartram ex Marshall from southeast North America and the Eocene fossil Nyssa eolignitica Berry from western Tennessee. Affinities …

A Mathematical Model For Antibiotic Resistance In A Hospital Setting With A Varying Population, Edward H. Snyder

Electronic Theses and Dissertations

Antibiotic-resistant bacteria(ARB) is causing increased health risk and cost to society. Mathematical models have been developed to study the transmission of resistant bacteria and the efficacy of preventive measures to slow its spread within a hospital setting. The majority of these models have assumed a constant total hospital population with the admission and discharge rates being equal throughout the duration. But a typical hospital population varies from day to day and season to season. In this thesis, we apply variable admission and discharge daily rates to existing deterministic and stochastic models which examine the transmission of single and dual resistant …

Compound Identification Using Penalized Linear Regression., Ruiqi Liu

Electronic Theses and Dissertations

In this study, we propose a new method for compound identification using penalized linear regression. Compound identification is often achieved by matching the experimental mass spectra to the mass spectra stored in a reference library based on mass spectral similarity. In the context of the linear regression, the response variable is an experimental mass spectrum (i.e., query) and all the compounds in the reference library are the independent variables. However, the number of compounds in the reference library is much larger than the range of m/z values so that the data become high dimensional data with suffering from singularity. For …

Evaluating The Use Of Autonomous Recording Units To Monitor Yellow Rails, Nelson's Sparrows, And Le Conte's Sparrows, Anna Marie Sidie-Slettedahl

Electronic Theses and Dissertations

Population status and habitat use of yellow rails (Coturnicops noveboracensis) (YERA), Nelson’s sparrows (Ammodramus nelsoni) (NESP), and Le Conte’s sparrows (Ammodramus leconteii) (LCSP) are poorly known, so systematic surveys of these elusive species are needed to inform conservation planning and guide management. A standardized protocol for monitoring secretive marsh birds exists (Conway 2009, 2011); however, these species call at night and may be missed during early-morning marsh bird surveys. I tested the effectiveness of autonomous recording units (ARUs) to survey these species by analyzing recorded vocalizations using bioacoustics software. I deployed 22 ARUs at 54 sites in northern Minnesota and …

X-Band Rapid-Scan Epr, Deborah Gale Mitchell

Electronic Theses and Dissertations

The advantages of rapid-scan EPR relative to CW and pulse techniques for samples with long longitudinal relaxation time T₁ (N_s⁰ defects in diamond, N@C₆₀, and amorphous hydrogenated silicon), heterogeneous samples (crystalline 1:1 α,γ-bisdiphenylene-β-phenylallyl (BDPA):benzene), lossy samples (aqueous nitroxyl radicals), and transient radicals (5-tert-butoxycarbonyl-5-methyl-1-pyrroline-N-oxide (BMPO)-superoxide adduct) were studied.

For samples with long relaxation times, CW (continuous wave) EPR is challenging due to power saturation and distortions from passage effects. In rapid-scan EPR, the field is swept through resonance in a time that is short relative to T₂. In rapid-scan EPR, the magnetic field is …

Seeded Propagation Of Tau Fibrils, Paul David Dinkel

Electronic Theses and Dissertations

In various neurodegenerative diseases, including Alzheimer's disease, progressive supranuclear palsy, Pick's disease, and corticobasal degeneration, the deposition of fibrils composed of misfolded tau protein is observed. Recent evidence suggests that tau fibrils transfer between cells and spread throughout the brain, underscoring the significance of fibril propagation.

Six tau isoforms exist in the adult human brain that can be grouped into 4-repeat (4R) tau and 3-repeat (3R) tau based on the presence or absence of the second of four microtubule binding repeats. We demonstrate in vitro that seeded fibril growth, a prerequisite for the spreading of the tau pathology, is crucially …

Ecology Of Glacial Relict Fishes In South Dakota's Sandhills Region, Eli Felts

Electronic Theses and Dissertations

Native stream fish zoogeography has changed substantially across North America during the last century as habitat degradation, stream fragmentation and introductions of nonnative species have led to numerous extinctions, extirpations and altered distributions. Insufficient information regarding imperiled species often results in reactive, rather than proactive, management, and knowledge of species status and ecology is critical in identifying conservation priorities. South Dakota populations of three dace species (northern redbelly dace Chrosomus eos, finescale dace Chrosomus neogaeus, and pearl dace Margariscus margarita) are relict of Pleistocene Glaciation and are isolated from the northern core of their distribution, but little information exists regarding …

Sagebrush Ecosystem Characterization, Monitoring, And Forecasting With Remote Sensing: Quantifying Future Climate And Wildlife Habitat Change, Collin G. Homer

Electronic Theses and Dissertations

Sagebrush (Artemisia spp.) ecosystems constitute the largest single North American shrub ecosystem and provide vital ecological, hydrological, biological, agricultural, and recreational ecosystem services. Disturbances continue to alter this ecosystem, with climate change possibly representing the greatest future disturbance risk. Improved ways to characterize and monitor gradual change in this ecosystem are vital to its future management. A new remote sensing sagebrush characterization approach was developed in Wyoming which integrates three scales of remote sensing to derive four primary continuous field components (bare ground, herbaceousness, litter, and shrub), and four secondary components (sagebrush, big sagebrush, Wyoming sagebrush, and shrub …