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Full-Text Articles in Life Sciences

Synergistic Impacts Of Climate Change And Human Induced Stressors On The Apalachicola Bay Food Web, Kira Allen Jan 2022

Synergistic Impacts Of Climate Change And Human Induced Stressors On The Apalachicola Bay Food Web, Kira Allen

Electronic Theses and Dissertations, 2020-

Apalachicola Bay, an estuary located in northwest Florida, is likely to experience an increase in climate change and human-induced stressors, such as sea level rise and changes in freshwater inflow, in the future. A coupled hydrodynamic and food web modeling approach was used to simulate future scenarios of low and high river flow and sea level rise in Apalachicola Bay from 2020 to 2049 and demonstrate the range of temporal and spatial changes in water temperature, salinity, fisheries species populations and the broader food web. Concurrent with model development, a survey of Apalachicola Bay stakeholders was conducted to assess stakeholder …


Investigation Of Catalysis Of Nitration By Cytochrome P450s, Lannika Johnson Jan 2022

Investigation Of Catalysis Of Nitration By Cytochrome P450s, Lannika Johnson

Honors Undergraduate Theses

TxtE is a protein related to cytochrome P450 enzymes, which catalyze a number of reactions that typically involve oxygen and not nitrogen. It has been discovered that TxtE can nitrate tryptophan through an unusual reaction in which it uses nitric oxide (NO) as a nitrogen donor to install the nitro group despite NO typically being considered toxic to bacteria. This project will determine if all cytochromes P450 can catalyze nitration as long as they are given NO. This will have an impact on understanding drug delivery and metabolism for which nitration is important.


Comparative Evaluation Of Assemblers For Metagenomic Data Analysis, Matheus Pavini Franco Ferreira Jan 2022

Comparative Evaluation Of Assemblers For Metagenomic Data Analysis, Matheus Pavini Franco Ferreira

Honors Undergraduate Theses

Metagenomics is a cultivation-independent approach for obtaining the genomic composition of microbial communities. Microbial communities are ubiquitous in nature. Microbes which are associated with the human body play important roles in human health and disease. These roles span from protecting us against infections from other bacteria, to being the causes of these diseases. A deeper understanding of these communities and how they function inside our bodies allows for advancements in treatments and preventions for these diseases. Recent developments in metagenomics have been driven by the emergence of Next-Generation Sequencing technologies and Third-Generation Sequencing technologies that have enabled cost-effective DNA sequencing …


Genomic Analysis Of Pollen Grains For Forensic Applications, Luz Kelley Jan 2022

Genomic Analysis Of Pollen Grains For Forensic Applications, Luz Kelley

Electronic Theses and Dissertations, 2020-

With over 300,000 plant species on the planet and more than 90% of them relying on pollen for reproduction, palynology, the study of pollen grains plays a vital role in many research fields. One of them is forensic palynology, which uses pollen as a proxy to link individuals or objects to a location or instance. It relies on the fact that (1) pollen is an ever-present feature of the environment; (2) different locations have different pollen signatures, allowing for inference related to spatial tracking; (3) plants bloom at different times, allowing for temporal inference; and (4) pollen is exceptionally durable …


Computational Methods To Analyze Next-Generation Sequencing Data In Genomics And Metagenomics, Saidi Wang Jan 2022

Computational Methods To Analyze Next-Generation Sequencing Data In Genomics And Metagenomics, Saidi Wang

Electronic Theses and Dissertations, 2020-

This thesis focuses on two important computational problems in genomics and metagenomics with the public available next-generation sequencing data. One is about gene regulation, for which we explore how distal regulatory elements may interact with the proximal regulatory elements. The other is about metagenomics, in which we study how to reconstruct bacterial strain genomes from shotgun reads. Studying gene regulation, especially distal gene regulation, is important because regulatory elements, including those in distal regulatory regions, orchestrate when, where and how much a gene is activated under every experimental condition. Their dysfunction results in various types of diseases. Moreover, the current …


"Reeling In" Juvenile Sportfish Through Coastal Habitat Restoration: Population, Community, And Trophic Responses In The Indian River Lagoon, Florida, Jennifer Loch Jan 2022

"Reeling In" Juvenile Sportfish Through Coastal Habitat Restoration: Population, Community, And Trophic Responses In The Indian River Lagoon, Florida, Jennifer Loch

Electronic Theses and Dissertations, 2020-

Coastal habitats provide crucial nursery habitat for predatory fishes, but they are in decline worldwide, impacting economically important fisheries. Habitat restoration can simultaneously mitigate the effects of habitat loss and benefit predators (e.g., sportfish), although this relationship is understudied. Here, the response of juvenile sportfish to oyster reef and living shoreline restoration is compared to controls in the Indian River Lagoon, Florida prior to and following restoration for up to three years through examination of community (diversity, assemblage), population (abundance), biometric (size, body condition), ontogenetic, and trophic (gut contents, stable isotopes) dynamics. Stable isotopes were used to quantify dietary history, …


Mathematical Model For Giraffe Population Dynamics, Huntir Bass Jan 2022

Mathematical Model For Giraffe Population Dynamics, Huntir Bass

Electronic Theses and Dissertations, 2020-

Since the 1980s the overall giraffe population has dropped at least 40% causing some researchers to label this rapid decline as the "Silent Extinction." Due to this plummet, understanding the behaviors of the giraffe population is absolutely necessary before they are on the brink of extinction. Through the usage of mathematical modeling methodologies, a general model is created to illustrate the relationship between juvenile and adult female giraffes through numerous interaction parameters. Variations on specific variables generate different simulations, which allows more biological accuracy. With each variation having an established coexistence equilibrium between the juvenile and adult female populations, the …


Investigation Of Dna Hybridization In Localized Systems In Close Proximity, Ashley M. Sewsankar Jan 2022

Investigation Of Dna Hybridization In Localized Systems In Close Proximity, Ashley M. Sewsankar

Honors Undergraduate Theses

Hybridization of two or more DNA or RNA strands is well documented for the process taking place with all strands free in solution or when one strand is immobilized on a substrate. This study contributes to the investigation of the hybridization process when two single DNA strands (ssDNA) are in close proximity. We took advantage of an X sensor in which hybridization of four DNA strands enables the formation of a DNA four-way junction (crossover or X) structure. We immobilized multiple layers of crossover structures to study its hybridization being triggered by short ssDNA coming from solution and further investigate …