Life Sciences Commons^™

Identification Of Compounds Causing Cellular Autofluorescence In Touch Samples, Elora C. Wall

Master of Science in Forensic Science Directed Research Projects

As DNA analysis has advanced and produced tests with higher sensitivities, attention has turned toward obtaining DNA profiles from cells left with fingermarks. Recent studies have reported that cells deposited within fingermarks can exhibit differences in autofluorescence emission in the ‘red’ region of the visible spectrum (e.g., between 650-670 nm), which can be used to differentiate contributor cell population and separate them before DNA profiles. Interestingly, this emission was not consistent to the individual day-to-day and likely not a genetically-controlled attribute of the contributor. Instead, this emission signature results from extended exposure of the skin to certain materials such as …

Impacts Of Beef Concentrated Animal Feeding Operations On Environmental Sustainability In The United States And Practices For Improvement, Laura Clark

VCU Phi Kappa Phi Award Winners

The geographic concentration of beef concentrated animal feeding operations [CAFOs] has changed the landscape of environmental sustainability for agriculture in the United States. As land availability has decreased, operations have struggled to maintain feasible practices to minimize environmental detriment. The United States Environmental Protection Agency [EPA] imposed rules to be followed as a means of mitigation, but the fast-paced rate of change minimizes effectiveness. The overall environmental sustainability of beef CAFOs has shifted from historical rates, leading to a need for reassessment. Part of this reassessment will include stronger environmental practices to be considered for implementation. I explored the role …

Application Of Optical Trapping To Obtain Single-Source Str Profiles From Forensically Relevant Body Fluid Mixtures With Modified Dna Analysis Workflow, Benjamin J. O'Brien

Master of Science in Forensic Science Directed Research Projects

Current methods of mixture separation in forensic DNA laboratories typically deconvolute the mixture after analysis using statistical analysis or probabilistic genotyping. To save time and effort of labs already backlogged, a method to separate mixtures on a cellular level before analysis needs to be developed. Optical trapping is a method that uses a focused 1064 nm laser to manipulate cells. Previous research has shown that approximately 50 spermatozoa or 15 leukocytes from a liquid sample are required to produce a full STR DNA profile. It was found that the number of spermatozoa required remains constant when the method of sample …

Firing Rate Dynamics In Recurrent Spiking Neural Networks With Intrinsic And Network Heterogeneity, Cheng Ly

Statistical Sciences and Operations Research Publications

Heterogeneity of neural attributes has recently gained a lot of attention and is increasing recognized as a crucial feature in neural processing. Despite its importance, this physiological feature has traditionally been neglected in theoretical studies of cortical neural networks. Thus, there is still a lot unknown about the consequences of cellular and circuit heterogeneity in spiking neural networks. In particular, combining network or synaptic heterogeneity and intrinsic heterogeneity has yet to be considered systematically despite the fact that both are known to exist and likely have significant roles in neural network dynamics. In a canonical recurrent spiking neural network model, …

Seeding The Universe With Life: Securing Our Cosmological Future, Michael Noah Mautner

Chemistry Publications

Life is unique in Nature, and for us, it is precious. Life is unique in its complex patterns, and in its purposeful self-continuation. We belong to life and share its drive for self-propagation. Belonging to life then implies a human purpose to secure, expand and propagate our family of gene/protein life.

This purpose is best achieved in space, where life has an immense future. We can start now to secure this future, by seeding with life new solar systems. New species can develop there into intelligent beings who will further expand life in the galaxy. Filling the universe with life …

Planetary Bioresources And Astroecology 1. Planetary Microcosm Bioassays Of Martian And Carbonaceous Chondrite Materials: Nutrients, Electrolyte Solutions, And Algal And Plant Responses, Michael Noah Mautner

Chemistry Publications

The biological fertilities of planetary materials can be assessed using microcosms based on materials in martian and carbonaceous chondrite meteorites. Their biological fertilities are rated based on soluble electrolyte nutrients, on the growth of mesophile and cold-tolerant algae, and of plant tissue cultures. The Murchison CM2 carbonaceous chondrite meteorite and DaG 476 martian shergottite contain high levels of water-extractable Ca, Mg, and SO4–S. The martian meteorites DaG 476 and EETA 79001 also contain high levels of extractable nutrients NO3–N (0.013–0.017 g kg⁻¹) and PO4–P (0.019–0.046 g kg⁻¹). The yields of most of the water-extractable …

Space-Based Genetic Cryoconservation Of Endangered Species, Michael Noah Mautner

Chemistry Publications

Genetic materials of endangered species must be maintained, for cryoconservation, permanently near liquid nitrogen temperatures below 77 K. Due to the instability of human institutions, permanent safety is best provided at storage sites that maintain passively the needed low temperatures, and provide barriers to access. The required conditions are available in permanently shaded polar lunar craters with equilibrium temperatures of 8 to 40 K, on the moons of Saturn, and unshielded storage satellites. A genetic depository can be incorporated readily into planned lunar programmes.