Life Sciences Commons^™

A Life Cycle Analysis Of Land Use In Us Pork Production, Greg Thoma, Marty Matlock, Ben Putman, Jasmina Burek

Food Systems

The goal of this study was to analyze land use in the production of US pork using Life Cycle Assessment (LCA). LCA is a comprehensive methodology for quantitatively analyzing potential environmental impacts associated with complex systems. Identification of processes contributing to high environmental impacts often highlights opportunities for gains in efficiency, which can increase the profitability and sustainability of US pork. The environmental impact category analyzed in this assessment was land use. After reviewing existing information regarding land use in agriculture and livestock production, analysis for US pork production was performed at two scales: cradle-to-grave and cradle-to-farm gate. The cradle-to-grave …

Effects Of Melatonin On Heartbeat And Possible Identification Of A Melatonin Receptor In Drosophila Melanogaster, Tricia L. Vankirk

Electronic Theses and Dissertations

Chapter 1 of this manuscript is a literature review that serves as an introduction to the entire dissertation. Chapter 2 examines the effects of the melatonin injection on heart rate and rhythmicity in Drosophila melanogaster Canton-S (wild-type) pupae and pupae bearing a variety of heart mutations. Chapter 3 investigates further the possible mechanisms of melatonin’s ability to increase heart rhythmicity without significantly affecting heart rate. A melatonin antagonist, luzindole; a high-affinity melatonin agonist, 2-iodomelatonin and RNAi techniques are used to identify a possible melatonin receptor in Drosophila melanogaster.

An appendix contains a previously published manuscript detailing experiments performed at the …

Evaluation Of Animal Model Research, Kenneth J. Shapiro

Kenneth J. Shapiro, PhD

It is argued that a concept of evaluation of animal models that is broader and more useful than validation is available. Productive generativity refers to the degree to which a model furthers understanding and leads to more-effective treatment interventions. Results of the application of this novel evaluative frame to several animal models of eating disorders show that this animal-based research has not been productive. The question of the relation between clinic and animal laboratory is discussed.

Training A New Trick Using No-Reward Markers: Effects On Dogs’ Performance And Stress Behaviors, Naomi Rotenberg

Theses and Dissertations

This study explored using no-reward markers (NRMs). Dogs were taught a novel trick. In the IG group dogs’ errors were ignored; in the NRM group they elicited a tone. Performance and stress were evaluated. IG dogs reached higher levels of performance, with no difference in the frequency of stress behaviors.

Production And Economic Efficiencies Of Intensified Cow-Calf Production Management Systems, Jason M. Warner

Department of Animal Science: Dissertations, Theses, and Student Research

The objective of four experiments conducted was to evaluate production and economic efficiencies of intensified cow-calf production management systems. The first experiment tested the effect of calf age at weaning on cow and calf performance and feed utilization at 2 locations. Body weight change from early to conventional weaning time was greater for early-weaned cows. Cow BCS and conception rates were not impacted by weaning. Calf BW at conventional weaning time was greater for conventionally-weaned calves than early-weaned calves at ARDC, but greater for early-weaned than conventionally-weaned calves at PHREC. Calf ADG per unit of total feed energy intake was …

Gopher Intrusion In Cropping Systems, Kenneth Romero, Paul Cardinalli

Agricultural Education and Communication

The purpose of this project was to determine if no till vs. till on gopher populations was more beneficial. Which is more efficient at controlling gophers and explain the damage they can cause and the diseases they can potentially vector. What are the different options of pest managements that have to be used when dealing with a gopher infestation in a tilled field vs. a no-tilled field.

Autonomic Nervous System Reactivity In A Free-Ranging Mammal: Effects Of Dominance Rank And Personality, Elodie F. Briefer, James A. Oxley, Alan G. Mcelligott

Ethology Collection

Modulation of the autonomic nervous system (ANS) activity allows animals to effectively respond to internal and external stimuli in everyday challenges via changes in, for example, heart and respiration rate. Various factors, ranging from social such as dominance rank to internal such as personality or affective states can impact animal physiology. Our knowledge of the combinatory effects of social and internal factors on ANS basal activity and reactivity, and of the importance that each factor has in determining physiological parameters, is limited, particularly in nonhuman, free-ranging animals. In this study, we tested the effects of dominance rank and personality (assessed …

Digestibility Of Two Complete Pelleted Diets By The Horse (Equus Caballus) As A Model Animal For Nondomestic Hindgut Fermenters, Emily M. Schwartz

Master's Theses

Estimating nutrient and energy requirements of exotic animals is a necessary component of nutrition management in zoos and other wildlife facilities. In the absence of species-specific data, domestic animal models are often referenced. Herbivorous hindgut fermenters, such as horses, zebra, and rhinoceros, rely on microbial fermentation in the cecum and colon to utilize dietary structural carbohydrates. The study objective was to measure the digestible energy of two (LOW, HIGH) complete pelleted diets by the horse as a model for nondomestic hindgut fermenters. Seven, individually housed, adult Quarter Horse (Equus caballus) geldings were assigned to one of two diets as 100% …

Maternal Effects And Offspring Behavior: Potential Contributors To The Lack Of Recruitment In Mississippi Gopher Tortoises (Gopherus Polyphemus), Aaron L. Holbrook

Master's Theses

Federally threatened in Mississippi, gopher tortoise (Gopherus polyphemus) populations within the state have strongly variable recruitment and are generally in decline. Hatching success is significantly lower in Mississippi than in any other part of the species’ range , and most hatchlings die within the first year. There are few refuges where survival and hatching success is high. Here I compare two populations that differ in recruitment and offspring survival for differences in corticosterone. Corticosterone is a hormone that influences both energy availability and is released in elevated levels during stressful events, like living in poor quality habitat. Prolonged corticosterone elevation …

Broiler Breeder Mating Behavior And The Effects Of Pre-Incubation Embryo Integrity Modifications On Broiler Performance, Emily Kathryn Lhamon

Graduate Theses and Dissertations

Modern broiler breeders have been observed displaying significantly less mating behaviors than their ancestral fowl. Effective mating behavior translates into fertile hatching eggs that ultimately become broilers processed for market. The poultry industry has made significant changes in genetics, environment, housing, and diets of commercial poultry. However, there are some concessions made in genetic selection and streamlined management to produce larger, fast-growing, efficient birds. Aggression in broiler breeder males is believed to be a sign of high fertility. However, highly aggressive males pose a threat to the welfare of their flock mates, and may also impede frequency of mating and …

Prevalence Of Tick-Borne Pathogens In Small Mammals And White-Tailed Deer In Southeast Nebraska, Tim Hotaling

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

The prevalence of tick-borne diseases has been increasing in the United States for the past couple decades. Studies have been conducted throughout the US identifying tick-borne disease pathogens as well as their hosts and prevalence. Research was conducted in Nebraska to determine the presence of some tick-borne disease pathogens, their vectors, and their hosts, with emphasis made on Borrelia spp., Rickettsia rickettsii, and Ehrlichia chaffeensis.

Small rodents in southeast Nebraska were trapped and sampled at eight study sites using live capture traps. Captured rodents were assessed for active parasitism by ticks which were collected and placed in alcohol. …

Failure Of A Heterologous Recombinant Sca5/Ompb Protein-Based Vaccine To Elicit Effective Protective Immunity Against Rickettsia Rickettsii Infections In C3h/Hen Mice, Sean P. Riley, Marissa M. Cardwell, Yvonne G. Chan, Ludovic Pruneau, Fabio Del Piero, Juan J. Martinez

Faculty Publications

Spotted fever group (SFG) rickettsial species are obligate intracellular tick-borne pathogens that are responsible for important human diseases. Previous reports have demonstrated the feasibility of using recombinant surface cell antigen Sca5/OmpB to elicit protective immunity against homologous challenges using murine models of Mediterranean spotted fever and Rocky Mountain spotted fever. In addition, the feasibility of generating cross-protective immunity against related rickettsial species has also been established, but the molecular basis for these phenomena was not explored. Here, we demonstrate that vaccination of C3H/HeN mice with a recombinant OmpB domain derived from Rickettsia conorii induced high titer humoral immune responses that …

Impact Of Urban Factors And Invasive Species On White-Footed Mouse (Peromyscus Leucopus) Habitat Use And Foraging Behavior In An Urban Forest Park., William Persons

Electronic Theses and Dissertations

White-footed mice (Peromyscus leucopus) select for areas of greater canopy cover at the macro scale, and for areas with more CWD at the micro-scale. White-footed mice potentially avoid habitats with higher invasive species richness. White-footed mice preferentially foraged under the honeysuckle canopy in response to changes in temperature and humidity. This study suggests that the interaction between P. leucopus and ground layer invasive species is complex, and that the effect of moonlight may be diminished in this urban park. Urban ecosystems demonstrate high levels of anthropogenic land-use change, modification of abiotic inputs, and altered disturbance regimes. These changes result …

Performance, Body Temperature And Blood Metabolites Of Feedlot Steers As Influenced By Environmental Conditions And Supplementation Of Zilpaterol Hydrochloride, Bradley M. Boyd

Department of Animal Science: Dissertations, Theses, and Student Research

Trial one was conducted at the United States Meat Animal Research Center (MARC) near clay center, NE during the summer of 2014. The objective of this trial was to measure the effects of supplementing zilpaterol hydrochloride (ZH) for the final 21 days of the finishing period, and shade, on performance, body temperature, respiration rate, and mobility of finishing beef steers. Feeding ZH increased hot carcass weight, dressing percent, longissimus muscle area, and reduce USDA yield grade. Shade did not affect steer performance and did not reduce body temperature. Zilpaterol hydrochloride increased respiration rate when compared to control cattle and had …

The Effect Of Alternative Fertilization And Application Time On Voluntary Intake Of Forages, Jessica Clark

Graduate Theses and Dissertations

Dairy slurry and poultry litter have increased in popularity as fertilizers in the agriculture industry. However, residual effects of these manures on voluntary intake of forages from subsequent harvests are not well known. The objectives of this two part study were to determine if moisture level of alfalfa silage and the use of dairy slurry as a fertilizer have an effect on intake and digestibility by sheep, and if forage species and the use of poultry litter as a fertilizer have an effect on intake by horses. Eighteen ewes (47.6 ± 5.34 kg) were used in experiment 1 and were …

The Human Intruder Test: An Anxiety Assessment In Rhesus Macaques (Macaca Mulatta), Emily J. Peterson

Masters Theses

The human intruder test (HIT) is a noninvasive tool widely used for assessing anxiety in rhesus macaques (Macaca mulatta). This thesis explores the HIT procedure and applies it to a population of monkeys with a self-injurious behavioral pathology. Individual variation on this test can be used to assess anxiety and temperament. The first experiment of this thesis applied two different procedures of the HIT to 17 monkeys at UMass. Monkeys displayed little response to the intruder, and no significant differences were detected for the two procedures. To determine whether these responses were unique to the UMass monkeys, their …

North American River Otter (Lontra Canadensis) Presence And Habitat Analysis In Florida As Compared To Historical Data, Samantha Wilber

USF Tampa Graduate Theses and Dissertations

North American river otters are considered common in the state of Florida, but their populations have not been studied since the 1980’s. Since that time, Florida’s human population has more than doubled, and many natural areas of Florida have been developed. The aim of this study was to determine the presence or absence of river otters at locations in Florida which they historically inhabited. Forty-six sample sites where otters were historically found were obtained from the Florida Museum of Natural History Mammals Master Database (FMNH MMD). These sites were condensed to two focus areas, in and around Alachua and Collier …

Predicting Toucan Locations In Panama Using Arcgis, Daniel J. Herrera

Geography: Student Scholarship & Creative Works

Toucans are omnivorous birds native to southern Latin America and South America. They are non-migratory, and their range is disputed among experts. In an attempt to develop a better understanding of the range and behavior of toucans, correlations between toucan presence and geographic features of the area were analyzed to create a location probability map.

The Physiological Consequences Of Crib-Biting In Horses In Response To An Acth Challenge Test, S. Briefer Freymond, D. Bardou, Elodie F. Briefer, R. Bruckmaier, N. Fouché, J. Fleury, A.-L. Maigrot, A. Ramseyer, K. Zuberbühler, I. Bachmann

Physiology Collection

Stereotypies are repetitive and relatively invariant patterns of behavior, which are observed in a wide range of species in captivity. Stereotypic behavior occurs when environmental demands produce a physiological response that, if sustained for an extended period, exceeds the natural physiological regulatory capacity of the organism, particularly in situations that include unpredictability and uncontrollability. One hypothesis is that stereotypic behavior functions to cope with stressful environments, but the existing evidence is contradictory. To address the coping hypothesis of stereotypies, we triggered physiological reactions in 22 horses affected by stereotypic behavior (crib-biters) and 21 non-crib-biters (controls), using an ACTH challenge test. …

Laterality Enhances Numerical Skills In The Guppy, Poecilia Reticulata, Marco Dadda, Christian Agrillo, Angelo Bisazza, Culum Brown

Sentience Collection

It has been hypothesized that cerebral lateralization can significantly enhance cognition and that this was one of the primary selective forces shaping its wide-spread evolution amongst vertebrate taxa. Here, we tested this hypothesis by examining the link between cerebral lateralization and numerical discrimination. Guppies, Poecilia reticulata, were sorted into left, right and non-lateralized groups using a standard mirror test and their numerical discrimination abilities tested in both natural shoal choice and abstract contexts. Our results show that strongly lateralized guppies have enhanced numerical abilities compared to non-lateralized guppies irrespective of context. These data provide further credence to the notion that …

A Tail Of Two Phages: Genomic And Functional Analysis Of Listeria Monocytogenes Phages Vb_Lmos_188 And Vb_Lmos_293 Reveal The Receptor-Binding Proteins Involved In Host Specificity, Aidan Casey, Kieran Jordan, Horst Neve, Aidan Coffey, Olivia Mcauliffe

Department of Biological Sciences Publications

The physical characteristics of bacteriophages establish them as viable candidates for downstream development of pathogen detection assays and biocontrol measures. To utilize phages for such purposes, a detailed knowledge of their host interaction mechanisms is a prerequisite. There is currently a wealth of knowledge available concerning Gram-negative phage-host interaction, but little by comparison for Gram-positive phages and Listeria phages in particular. In this research, the lytic spectrum of two recently isolated Listeria monocytogenes phages (vB_LmoS_188 and vB_LmoS_293) was determined, and the genomic basis for their observed serotype 4b/4e host-specificity was investigated using comparative genomics. The late tail genes of these …

Economic Contribution Of Agriculture And Food To Arkansas' Gross Domestic Product 1997-2013, Leah English, Jennie Popp, Wayne Miller

Research Reports and Research Bulletins

Agricultural production, processing, and retail industries are major contributors to the Arkansas economy in terms of GDP. Agriculture contributes to the economy through direct agricultural production, value-added processing, and agricultural retail activities, and it also plays an important role through its interactions with other sectors. The use of non-agricultural goods and services as inputs into the agricultural sector promotes diversified growth in Arkansas’ economy; thus agriculture remains a vital part of Arkansas’ economy. This report: 1) compares the relative size of the Agriculture and Food Sector in Arkansas with those of neighboring states; 2) provides an overview of Arkansas’ economy …

An Analysis Of The Characteristics And Practices Of Selected Alabama Small Livestock Producers: A Focus On Economics And Marketing, Jannette R. Bartlett, Nii O. Tackie, Mst Nusrat Jahan, Akua Adu-Gyamfi

Professional Agricultural Workers Journal

Abstract

The study examined the characteristics and practices of small livestock producers, focusing on economics and marketing. Data were obtained from a convenience sample of 121 small producers from several South Central Alabama counties, and were analyzed using descriptive statistics, including chi-square tests. The socioeconomic characteristics reflected a higher proportion of part-time farmers; a higher proportion with at most a two-year/technical degree or some college education; and a higher proportion with $40,000 or less annual household income. A majority had been farming more than thirty years, and most had small herds. Also, very few made profits; many sold animals live …

Social Learning: Parents May Not Always Know Best, Simon C. Griffith, Culum Brown

Social Cognition Collection

The efficiency with which animals learn new skills depends on their ability to choose good tutors. A new study shows that early-life stress causes young zebra finches to switch tutor preference from parents to unrelated adults.

Pain In Aquatic Animals, Lynne U. Sneddon

Lynne Sneddon, PhD

Recent developments in the study of pain in animals have demonstrated the potential for pain perception in a variety of wholly aquatic species such as molluscs, crustaceans and fish. This allows us to gain insight into how the ecological pressures and differential life history of living in a watery medium can yield novel data that inform the comparative physiology and evolution of pain. Nociception is the simple detection of potentially painful stimuli usually accompanied by a reflex withdrawal response, and nociceptors have been found in aquatic invertebrates such as the sea slug Aplysia. It would seem adaptive to have a …

What Do Zebrafish Want? Impact Of Social Grouping, Dominance And Gender On Preference For Enrichment, Paul Schroeder, Soffia Jones, Iain S. Young, Lynne U. Sneddon

Lynne Sneddon, PhD

Although environmental enrichment is known to improve laboratory rodent wellbeing and enhance scientific data collection, relatively little is known with regards to the type of enrichment that might be useful for zebrafish (Danio rerio). Therefore, this study explored if zebrafish displayed preferences for a range of enrichments, including substrates, artificial plants, combinations thereof and airstones. Tanks divided into two compartments containing different enrichment cues were used to determine the preferences of zebrafish housed in pairs and groups of eight. When comparing time spent in enriched versus barren compartments, dominant individuals in a pair displayed a preference for substrate and behaviourally …

Expression Of Emotional Arousal In Two Different Piglet Call Types, Linhart Pavel, Victoria F. Ratcliffe, David Reby, Marek Špinka

Bioacoustics Collection

Humans as well as many animal species reveal their emotional state in their voice. Vocal features show strikingly similar correlation patterns with emotional states across mammalian species, suggesting that the vocal expression of emotion follows highly conserved signalling rules. To fully understand the principles of emotional signalling in mammals it is, however, necessary to also account for any inconsistencies in the way that they are acoustically encoded. Here we investigate whether the expression of emotions differs between call types produced by the same species. We compare the acoustic structure of two common piglet calls—the scream (a distress call) and the …

Investigation Of Van Gogh-Like 2 Mrna Regulation And Localisation In Response To Nociception In The Brain Of Adult Common Carp (Cyprinus Carpio), Siobhan C. Reilly, Anja Kipar, David J. Hughes, John P. Quinn, Andrew R. Cossins, Lynne U. Sneddon

Lynne Sneddon, PhD

The Van Gogh-like 2 (vangl2) gene is typically associated with planar cell polarity pathways, which is essential for correct orientation of epithelial cells during development. The encoded protein of this gene is a transmembrane protein and is highly conserved through evolution. Van Gogh-like 2 was selected for further study on the basis of consistent regulation after a nociceptive stimulus in adult common carp and rainbow trout in a microarray study. An in situ hybridisation was conducted in the brain of mature common carp (Cyprinus carpio), 1.5 and 3 h after a nociceptive stimulus comprising of an acetic acid injection to …

Three Rs Approaches In The Production And Quality Control Of Fish Vaccines, Paul J. Midtlyng, Coenraad F.M. Hendriksen, Elisabeth Balks, Lukas Bruckner, Lawrence Elsken, Øystein Evensen, Kjetil Fyrand, Allison Guy, Marlies Halder, Penny Hawkins, Gunn Kisen, Anne Berit Romstad, Kira Salonius, Patrick Smith, Lynne U. Sneddon

Lynne Sneddon, PhD

The workshop on Three Rs Approaches in the Production and Quality Control of Fish Vaccines aimed a) to identify animal tests currently stipulated for the production and quality control of fish vaccines and to highlight animal welfare concerns associated with these tests; b) to identify viable options to replace, reduce, and refine animal use for fish vaccine testing; and c) to discuss the way forward and set out how the Three Rs may be implemented without jeopardizing the quality of the vaccines. The workshop participants -- experts from academia, regulatory authorities, a scientific animal welfare organization, and the fish vaccine …

Suitability Of Great South Bay, New York To Blooms Of Pfiesteria Piscicida And P. Shumwayae Prior To Superstorm Sandy, October 29, 2012, Pawel Tomasz Zablocki

Theses and Dissertations

Pfiesteria piscicida and P. shumwayae are toxic dinoflagellates implicated in massive fish kills in North Carolina and Maryland during 1990s. A set of physical, chemica l, and biological factors influence population dynamics of these organisms. This study employs i nformation gathered from relevant literature on temperature, salinity, dissolved oxygen, pH, turbulent mixing, and dissolved nutrients, bacteria, algae, microzooplankton, mesozooplankton, bivalve m ollusks, finfish, and other toxic dinoflagellates, which influence Pfiesteria population dynamics. The research focused on whether conditions in the Great South Bay, Long Island, New York were suitable to blooms of Pfiesteria species prior to the passage of superstorm …