Life Sciences Commons^™

The Role Of Water Motion In Algal Reproduction, Richard Gordon

Electronic Theses and Dissertations

Environmental conditions, such as water motion, can influence fertilization success and spore dispersal in marine algae. Previous studies on fucoid algae showed that gamete release is restricted to, or enhanced by, periods of low water motion. Few other algal taxa have been investigated, however, including species with an alternation of generations. I investigated gamete and spore release in the macroalgae Alaria esculenta and Ulva lactuca, as well as in the diatom Pseudo-nitachia multiseries to determine if water motion is inhibitory or stimulates propagule production and release. I used orbital shakers to simulate water motion; these were interspersed with stationary platforms …

Gametogenic Cycles Of Marine Mussels, Mytilus Edulis And Mytilus Trossulus, In Cobscook Bay, Maine, Aaron P. Maloy

Electronic Theses and Dissertations

The Mytilus edulis species complex includes three smooth-shelled blue mussels, M. edulis (Linnaeus 1 75 8), M trossulus (Gould 1 850), and M galloprovincialis (Lamarck 18 19). When any two of theses species occur sympatrically, hybridization and backcrossing of hybrid and parental genotypes is evident. Despite introgression of genes between taxa their genetic integrity is maintained. To test the hypothesis that a temporal variation in species-specific spawning times is the mechanism limiting hybridization and maintaining genetic integrity in a M edulis and M. trossulus hybrid zone in eastern Maine, mussels were sampled on monthly to semi-monthly intervals throughout 2000 from …

Fall 2001, Nsu Oceanographic Center

Currents

No abstract provided.

Emersion Stress In Intertidal Seaweeds: Role Of Active Oxygen, Ian R. Davison

University of Maine Office of Research Administration: Grant Reports

The study will examine stress-tolerance in two major groups of perennial intertidal macroalgae, the red and brown seaweeds. The research will test the hypothesis that active oxygen is involved in emersion stress of intertidal seaweeds. Damage due to active oxygen will be determined in stress-tolerant and stress- susceptible species exposed to emersion stress by measuring the peroxidation of membrane lipids. Plants will be grown in laboratory culture under conditions that increase their ability to withstand emersion stress. If the research hypothesis is correct, increases in stress tolerance should be associated with increased levels of antioxidants and/or protective enzymes. The proposed …

School Of Marine Sciences / Darling Marine Center, Kevin J. Eckelbarger

University of Maine Office of Research Administration: Grant Reports

Over the last six years, the University of Maine has made an unprecedented investment in its marine laboratory, the Darling Marine Center to benefit both University faculty and visiting researchers and their students. Facility improvements include many new laboratory and offices spaces, more research instrumentation, and basic support facilities such as a dining hall and new classrooms. The inauguration of a Visiting Investigation Program in 1991, the expansion of educational offerings, and the growth of a large undergraduate internship program, have resulted in a population explosion that shows no sign of abating. To set priorities for improvements, the University has …

Trichodesmium Spp.: Numerical Studies Of Resource Competition, Carbohydrate Ballasting, And Remote-Sensing Reflectance, Tonya Denise Clayton

OES Theses and Dissertations

In recent years, a new appreciation for the role of diazotrophy in the oceans has emerged. This dissertation reports on three modeling studies designed to investigate ecological processes associated with Trichodesmium spp., the most conspicuous marine diazotroph: (1) characterization of a generalized model Trichodesmium and issues of macronutrient resource competition; (2) carbohydrate ballasting by Trchodesmium and implications for the formation of surface accumulations; and (3) the vertical distribution of Trichodesmium and implications for detection from space.

The first study focuses on issues of nitrogen and phosphorus competition and ecosystem structure. It utilizes a simple ecosystem model that includes dissolved nitrogen …

Spring/Summer 2001, Nsu Oceanographic Center

Currents

No abstract provided.

The Implications Of The Target-Area Hypothesis On The Population Dynamics Of The Spotted Spiny Lobster, Panulirus Guttatus, Denice N. Robertson

Biological Sciences Theses & Dissertations

The target-area hypothesis, based on the theory of island biogeography, predicts that larger islands are more effective at intercepting passive immigrants. Most marine invertebrates have meroplanktonic larvae and open population dynamics, so immigration to populations in isolated benthic habitats is primarily by pelagic larval recruits. Thus, recruitment to isolated habitat “islands” may be more continuous and predictable on large islands than on small ones. Consequently, populations on large islands should not only be larger than those on small islands, but should also have more evenly distributed size structures. These differences in size structure among populations in isolated habitats of differing …

Uptake Of Dissolved Organic Selenides By Marine Phytoplankton, Stephen B. Baines, Nicholas S. Fisher, Martina A. Doblin, Gregory A. Cutter

OES Faculty Publications

Se is present in multiple oxidation states in nature, each of which has unique chemical and biological reactivities. As a consequence, the rate of Se incorporation into food webs or its role as either a limiting nutrient or a toxic substance is a function of complex biogeochemistry. In particular, little is understood about the accumulation of dissolved organic selenides by phyto- or bacterioplankton. We assessed the bioavailability of dissolved organic selenides to marine and estuarine phytoplankton by presenting various algal species with filtered lysates of the diatom, Thalassiosira pseudonana, grown on media amended with radiolabeled selenite (⁷⁵Se[IV]). …

Age, Growth, And Mortality Of Atlantic Croaker, Micropogonias Undulatus, In The Chesapeake Bay Region, John R. Foster

Dissertations, Theses, and Masters Projects

No abstract provided.

Spatial And Temporal Variability Of Physical And Biological Mixing In The York River Subestuary, Tara A. Kniskern

Dissertations, Theses, and Masters Projects

No abstract provided.

Vitellogenin -- A Biomarker Of Exposure To Environmental Estrogens For Mummichog (Fundulus Heteroclitus) From A Creosote-Contaminated Site?, Sara Mirabilio

Dissertations, Theses, and Masters Projects

Vitellogenin (VTG) is widely used as a biomarker for environmental estrogens and reproductive disruption in fish. Vitellogenesis is the process by which yolk is formed. The endpoint is most sensitive in male fish where vitellogenesis is an abnormal process. Research examining effects of environmental mixtures of chemicals (e.g. creosote) on vitellogenesis is limited. This study examines plasma VTG expression in both male and female mummichog, Fundulus heteroclitus, collected from a creosote-contaminated site and two reference sites in lower Chesapeake Bay, USA, and in wild-caught male reference fish exposed in the laboratory to creosote-contaminated sediment. Further, this study uses tissue somatic …

Use Of Pop-Up Satellite Tag Technology To Estimate Survival Of Blue Marlin (Makaira Nigricans) Released From Pelagic Longline Gear, David Kerstetter

Dissertations, Theses, and Masters Projects

No abstract provided.

Growth Patterns Of Three Species Of Catfish (Ictaluridae) From Three Virginia Tributaries Of The Chesapeake Bay, William J. Connelly

Dissertations, Theses, and Masters Projects

No abstract provided.

A Descriptive Study Of The Reproductive Biology Of The Veined Rapa Whelk (Rapana Venosa) In The Chesapeake Bay, Erica S. Westcott

Dissertations, Theses, and Masters Projects

No abstract provided.

Reproductive Biology Of Atlantic Croaker, Micropogonias Undulatus, In The Chesapeake Bay, Elizabeth Shaw Watkins

Dissertations, Theses, and Masters Projects

No abstract provided.

Heat Shock Protein (Hsp70) Response In The Eastern Oyster, Crassostrea Virginica, Exposed To Various Contaminants (Pahs, Pcbs And Cadmium), Luis A. Cruz Rodriguez

Dissertations, Theses, and Masters Projects

The stress protein response has been proposed as a general indicator of exposure to stress as their expression might integrate overall biological impact and interactions among multiple stressors. as a marker of contaminant effects, a major advantage is the premise of higher sensitivity over other indices such as condition index, scope for growth and survival. Laboratory exposure to suspended field contaminated sediments (SFCS) elicited a stress protein response (HSP70) in the eastern oyster, ( Crassostrea virginica). The stress response probably resulted from the combined effect of various contaminants including PAHs, metals and PCBs. Exposure to 1--2g suspended clay particles spiked …

Molecular Characterization Of Cultured Perkinsus Marinus Isolates, Gwynne D. Brown

Dissertations, Theses, and Masters Projects

Perkinsus marinus is the causative agent of the oyster disease Dermo in the eastern oyster, Crassostrea virginica. In vitro propagation of the parasite has led to the establishment of multiple isolates by several investigators. Little work, however, has been done to characterize different isolates. In this study multiple isolates were examined for genetic and biochemical diversity. at two loci, the ITS region and the ATAN region, there was as much intra-isolate genetic variation among DNA sequences of some isolates as there was inter-isolate variation. Variation was also observed at a third loci, a subtilisin-like serine protease gene. This is the …

The 6th International Conference And Workshop On Lobster Biology And Management: An Introduction, Mark J. Butler Iv

Biological Sciences Faculty Publications

Every three years or so, the International Conference and Workshop on Lobster Biology and Management (ICWL) brings together lobster scientists, fishery managers, and industry representatives from around the world for a week of scientific presentations, workshops, and discussions on lobster biology and management. The first ICWL was held in Perth, Australia, in January 1977. Its purpose was to bring together a small group of lobster researchers from the USA and Australia to discuss common issues and themes. That initial workshop spawned a continuing series of meetings that have become the international lobster conference for scientists—the equivalent of an international congress …

Top-Down Impact Through A Bottom-Up Mechanism. In Situ Effects Of Limpet Grazing On Growth, Light Requirements And Survival Of The Eelgrass Zostera Marina, Richard C. Zimmerman, Diana L. Steller, Donald G. Kohrs, Randall S. Alberte

OES Faculty Publications

Temporal changes in abundance, size, productivity, resource allocation and light requirements of a subtidal eelgrass (Zostera marina L.) population were followed for 2 yr after the September 1993 appearance of a previously rare oval form of the commensal limpet Tectura depicta (Berry) in Monterey Bay, California, USA, By exclusively targeting the epidermis, limpet grazing impaired photosynthetic performance but left respiratory demand, meristematic growth and more than 90 % of the leaf biomass intact, The resulting low P:R ratios of grazed plants raised the light requirements for the maintenance of positive carbon balance almost 2-fold relative to healthy ungrazed plants …

Control Of Phytoplankton Growth By Iron And Silicic Acid Availability In The Subantarctic Ocean: Experimental Results From The Saz Project, D. A. Hutchins, Peter N. Sedwick, G. R. Ditullio, P. W. Boyd, B. Queguiner, F. B. Griffiths, C. Crossley

OES Faculty Publications

Subantarctic Southern Ocean surface waters in the austral summer and autumn are characterized by high concentrations of nitrate and phosphate but low concentrations of dissolved iron (Fe, similar to0.05 nM) and silicic acid (Si, <1 muM). During the Subantarctic Zone AU9706 cruise in March 1998 we investigated the relative importance of Fe and Si in controlling phytoplankton growth and species composition at a station within the subantarctic water mass (46.8degreesS, 142degreesE) using shipboard bottle incubation experiments. Treatments included unamended controls; 1.9 nM added iron (+Fe); 9 muM added silicic acid (+Si); and 1.9 nM addediron plus 9 muM added silicic acid (+Fe+Si). We followed a detailed set of biological and biogeochemical parameters over 8 days. Fe added alone clearly increased community growth rates and nitrate drawdown and altered algal community composition relative to control treatments. Surprisingly, small, lightly silicified pennate diatoms grew when Fe was added either with or without Si, despite the extremely low ambient silicic acid concentrations. Pigment analyses suggest that lightly silicified chrysophytes (type 4 haptophytes) may have preferentially responded to Si added either with or without Fe. However, for many of the parameters measured the +Fe+Si treatments showed large increases relative to both the +Fe and +Si treatments. Our results suggest that iron is the proximate limiting nutrient for chlorophyll production, photosynthetic efficiency, nitrate drawdown, and diatom growth, but that Si also exerts considerable control over algal growth and species composition. Both nutrients together are needed to elicit a maximum growth response, suggesting that both Fe and Si play important roles in structuring the subantarctic phytoplankton community.

Control Of Phytoplankton Growth By Iron Supply And Irradiance In The Subantarctic Southern Ocean: Experimental Results From The Saz Project, P. W. Boyd, A. C. Crossley, G. R. Ditullio, F. B. Griffiths, D. A. Hutchins, B. Queguiner, Peter N. Sedwick, T. W. Trull

OES Faculty Publications

The influence of irradiance and Fe supply on phytoplankton processes was studied, north (47°S, 142°E) and south (54°S, 142°E) of the Subantarctic Front in austral autumn (March 1998). At both sites, resident cells exhibited nutrient stress (F_v/F_m 0 at 47°S and 9% I₀ at 54°S because of MLDs of 40 (47°S) and 90 m (54°S), when these stations were occupied. The greater MLD at 54°S is reflected by tenfold higher cellular chlorophyll a levels in the resident phytoplankton. In the 47°S experiment, chlorophyll a levels increased to >1 μg/L^-1 only in the high-Fe treatments, regardless …

Effects Of Iron, Silicate, And Light On Dimethylsulfoniopropionate Production In The Australian Subantarctic Zone, G. R. Ditullio, P. N. Sedwick, D. R. Jones, P. W. Boyd, A. C. Crossley, D. A. Hutchins

OES Faculty Publications

Shipboard bottle incubation experiments were performed to investigate the effects of iron, light, and silicate on algal production of particulate dimethylsulfoniopropionate (DMSP_p) in the Subantarctic Zone (SAZ) south of Tasmania during March 1998. Iron enrichment resulted in threefold to ninefold increases in DMSP_p concentrations relative to control treatments, following 7 and 8-day incubation experiments. Additions of Fe and Si preferentially stimulated the growth of lightly-silicified pennate diatoms and siliceous haptophytes, respectively, to which we attribute the increased DMSP_p production in the incubation bottles. Both of these algal groups were previously believed to be low DMSP_{p …}

Holocene Sediment Records From The Continental Shelf Of Mac. Robertson Land, East Antarctica, Peter N. Sedwick, Peter T. Harris, Lisette G. Robertson, Gary M. Mcmurtry, Maximilian D. Cremer, Philip Robinson

OES Faculty Publications

Geochemical records are presented for five sediment cores from basins on the continental shelf of Mac. Robertson Land, East Antarctica. The cores contain 2-4 m thick sequences of hemipelagic, siliceous mud and ooze (SMO) deposited under seasonally open marine conditions. The inner and middle shelf SMO sequences are massive dark olive green material, whereas the outer shelf SMO sequences are dark olive material interspersed with light olive green layers similar to1-10 cm thick. The biogenic material is dominated by marine diatoms including Fragilariopsis curta, Fragilariopsis cylindrus, and Chaetoceros spp. in the dark-colored SMO and Corethron criophilum in the …

Nursery Delineation, Habitat Utilization, Movements, And Migration Of Juvenile Carcharhinus Plumbeus In Chesapeake Bay, Virginia, United States Of America, R. Dean. Grubbs

Dissertations, Theses, and Masters Projects

Chesapeake Bay is possibly the largest summer nursery for Carcharhinus plumbeus in the western Atlantic. Longline sampling conducted from 1990--1999 was used to delineate this nursery spatially and temporally. Catch data from 83 longline stations sampled throughout the Virginia Chesapeake Bay were analyzed as a function of nine physical and environmental variables to delineate this nursery spatially. Tree-based models determined which variables best discriminated between stations with high and low catches and indicated that complex distribution patterns could be adequately modeled with few variables. The highest abundance of juvenile sharks was predicted where salinity was greater than 20.5 and depth …

The Impact Of Marine Reserves On Exploited Species With Complex Life Histories: A Modeling Study Using The Caribbean Spiny Lobster In Exuma Sound, Bahamas, William T. Stockhausen

Dissertations, Theses, and Masters Projects

Most benthic invertebrates and reef-associated fish undergo a dispersive, planktonic larval stage prior to settlement and metamorphosis into the juvenile and adult stages. In some species, settlement may be decoupled from adult abundance at local spatial scales if hydrodynamic conditions or larval behavior do not promote local retention. Similarly, spatial variability in postsettlement mortality or secondary dispersal by juveniles and adults may decouple spatial patterns of adult abundance from those of settlement. as a consequence, spatial patterns of settlement and adult abundance may be functionally related in a complex fashion. Whether biotic/environmental factors control spatial patterns of abundance may have …

Life History Attributes Of Mid-Atlantic Menidia Menidia (Pisces: Atherinidae) And A Comparison With Northern (Massachusetts) And Southern (South Carolina) Populations, Richard K. Holmquist

Dissertations, Theses, and Masters Projects

No abstract provided.

Morphological Variation Of Three Populations Of The Veined Rapa Whelk, Rapana Venosa, An Invasive Predatory Gastropod Species, Rebecca A. Green

Dissertations, Theses, and Masters Projects

No abstract provided.

Evaluation Of Opercular Bones For Aging Eight Species Of Chesapeake Bay Fishes, Ann M. Sipe

Dissertations, Theses, and Masters Projects

No abstract provided.