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Molecular Genetics Of The Caenorhabditis Elegans Heterochronic Gene Lin-14, Gary Ruvkun, Victor Ambros, Alan Coulson, Robert Waterston, John Sulston, H. Horvitz
Molecular Genetics Of The Caenorhabditis Elegans Heterochronic Gene Lin-14, Gary Ruvkun, Victor Ambros, Alan Coulson, Robert Waterston, John Sulston, H. Horvitz
Victor R. Ambros
We describe a general strategy for the genetic mapping in parallel of multiple restriction fragment length polymorphism (RFLP) loci. This approach allows the systematic identification for cloning of physical genetic loci within about 100 kb of any gene in Caenorhabditis elegans. We have used this strategy of parallel RFLP mapping to clone the heterochronic gene lin-14, which controls the timing and sequence of many C. elegans postembryonic developmental events. We found that of about 400 polymorphic loci in the C. elegans genome associated with the Tc1 family of repetitive elements, six are within 0.3 map unit of lin-14. The three …