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Characterization Of Steroid-17,20-Desmolase And 20Α-Hydroxysteroid Dehydrogenase From Clostridium Scindens, Amy Elizabeth Krafft
Characterization Of Steroid-17,20-Desmolase And 20Α-Hydroxysteroid Dehydrogenase From Clostridium Scindens, Amy Elizabeth Krafft
Theses and Dissertations
Clostridium scindens is an obligate anaerobe isolated from the normal intestinal flora of man. It is the only bacterium known at present to synthesize both steroid-17,20-desmolase and 20α-hydroxysteroid dehydrogenase (ZOα-HSDH). This study was undertaken to characterize these neutral steroid transforming reactions in extracts of C. scindens; to purify and characterize the enzymes responsible for side-chain cleavage and 20α- hydroxysteroid oxidoreduction and to clone the genes encoding these enzymes with a view towards studying the mechanism of induction by steroids in a prokaryotic system. Both enzymes were found to be co-inducible in cells cultured in the presence of 17α-hydroxysteroids. In cell …