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Full-Text Articles in Life Sciences
Nonspecific Interactions Alter Lipopolysaccharide Patterns And Protein Mobility On Sodium Dodecyl Sulfate Polyacrylamide Gels, Aidong Yuan, R L. Pardy, Catherine P. Chia
Nonspecific Interactions Alter Lipopolysaccharide Patterns And Protein Mobility On Sodium Dodecyl Sulfate Polyacrylamide Gels, Aidong Yuan, R L. Pardy, Catherine P. Chia
Papers in Microbiology
In testing whether bacterial lipopolysaccharide (LPS) was a natural substrate for an esterase from the soil amebae Dictyostelium discoideum, we observed altered banding patterns of the LPS and changed protein mobility on sodium dodecyl sulfate (SDS) polyacrylamide gels after incubation of LPS with the enzyme. The initial interpretation of these results was that the enzyme had removed ester-linked acyl chains from the LPS, leading to a change in its migration on gels. However, esterase inactivated by treatment with either dithiothreitol (DTT), heat, or SDS generated the same mobility shifts. Bovine serum albumin (BSA) also induced the same change in …