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Full-Text Articles in Life Sciences
A Genetic System For Clostridium Ljungdahlii: A Chassis For Autotrophic Production Of Biocommodities And A Model Homoacetogen, Ching Leang, Toshiyuki Ueki, Kelly Nevin, Derek R. Lovley
A Genetic System For Clostridium Ljungdahlii: A Chassis For Autotrophic Production Of Biocommodities And A Model Homoacetogen, Ching Leang, Toshiyuki Ueki, Kelly Nevin, Derek R. Lovley
Kelly Nevin
Methods for genetic manipulation of Clostridium ljungdahlii are of interest because of the potential for production of fuels and other biocommodities from carbon dioxide via microbial electrosynthesis or more traditional modes of autotrophy with hydrogen or carbon monoxide as the electron donor. Furthermore, acetogenesis plays an important role in the global carbon cycle. Gene deletion strategies required for physiological studies of C. ljungdahlii have not previously been demonstrated. An electroporation procedure for introducing plasmids was optimized, and four different replicative origins for plasmid propagation in C. ljungdahlii were identified. Chromosomal gene deletion via double-crossover homologous recombination with a suicide vector …
Improved Cathode Materials For Microbial Electrosynthesis, Tian Zhang, Huarong Nie, Timothy S. Bain, Haiyun Lu, Mengmeng Cui, Oona L. Snoeyenbos-West, Ashley E. Franks, Kelly Nevin, Thomas P. Russell, Derek R. Lovley
Improved Cathode Materials For Microbial Electrosynthesis, Tian Zhang, Huarong Nie, Timothy S. Bain, Haiyun Lu, Mengmeng Cui, Oona L. Snoeyenbos-West, Ashley E. Franks, Kelly Nevin, Thomas P. Russell, Derek R. Lovley
Kelly Nevin
Microbial electrosynthesis is a promising strategy for the microbial conversion of carbon dioxide to transportation fuels and other organic commodities, but optimization of this process is required for commercialization. Cathodes which enhance electrode–microbe electron transfer might improve rates of product formation. To evaluate this possibility, biofilms of Sporomusa ovata, which are effective in acetate electrosynthesis, were grown on a range of cathode materials and acetate production was monitored over time. Modifications of carbon cloth that resulted in a positive-charge enhanced microbial electrosynthesis. Functionalization with chitosan or cyanuric chloride increased acetate production rates 6–7 fold and modification with 3-aminopropyltriethoxysilane gave rates …
Anaerobic Benzene Oxidation By Geobacter Species, Tian Zhang, Timothy S. Bain, Kelly Nevin, Melissa A. Barlett, Derek R. Lovley
Anaerobic Benzene Oxidation By Geobacter Species, Tian Zhang, Timothy S. Bain, Kelly Nevin, Melissa A. Barlett, Derek R. Lovley
Kelly Nevin
The abundance of Geobacter species in contaminated aquifers in which benzene is anaerobically degraded has led to the suggestion that some Geobacter species might be capable of anaerobic benzene degradation, but this has never been documented. A strain of Geobacter, designated strain Ben, was isolated from sediments from the Fe(III)-reducing zone of a petroleum-contaminated aquifer in which there was significant capacity for anaerobic benzene oxidation. Strain Ben grew in a medium with benzene as the sole electron donor and Fe(III) oxide as the sole electron acceptor. Furthermore, additional evaluation of Geobacter metallireducens demonstrated that it could also grow in benzene-Fe(III) …
Interspecies Electron Transfer Via H2 And Formate Rather Than Direct Electrical Connections In Co-Cultures Of Pelobacter Carbinolicus And Geobacter Sulfurreducens, Amelia-Elena Rotaru, Pravin M. Shrestha, Fanghua Liu, Toshiyuki Ueki, Kelly Nevin, Zarath M. Summers, Derek R. Lovley
Interspecies Electron Transfer Via H2 And Formate Rather Than Direct Electrical Connections In Co-Cultures Of Pelobacter Carbinolicus And Geobacter Sulfurreducens, Amelia-Elena Rotaru, Pravin M. Shrestha, Fanghua Liu, Toshiyuki Ueki, Kelly Nevin, Zarath M. Summers, Derek R. Lovley
Kelly Nevin
Direct interspecies electron transfer (DIET) is an alternative to interspecies H2/formate transfer as a mechanism for microbial species to cooperatively exchange electrons during syntrophic metabolism. To understand what specific properties contribute to DIET, studies were conducted with Pelobacter carbinolicus, a close relative of Geobacter metallireducens, which is capable of DIET. P. carbinolicus grew in co-culture with Geobacter sulfurreducens with ethanol as electron donor and fumarate as electron acceptor, conditions under which G. sulfurreducens formed direct electrical connections with G. metallireducens. In contrast to the cell aggregation associated with DIET, P. carbinolicus and G. sulfurreducens did not aggregate. Attempts to initiate …
Promoting Direct Interspecies Electron Transfer With Activated Carbon, Fanghua Liu, Amelia-Elena Rotaru, Pravin M. Shrestha, Nikhil S. Malvankar, Kelly Nevin, Derek R. Lovley
Promoting Direct Interspecies Electron Transfer With Activated Carbon, Fanghua Liu, Amelia-Elena Rotaru, Pravin M. Shrestha, Nikhil S. Malvankar, Kelly Nevin, Derek R. Lovley
Kelly Nevin
Granular activated carbon (GAC) is added to methanogenic digesters to enhance conversion of wastes to methane, but the mechanism(s) for GAC's stimulatory effect are poorly understood. GAC has high electrical conductivity and thus it was hypothesized that one mechanism for GAC stimulation of methanogenesis might be to facilitate direct interspecies electron transfer (DIET) between bacteria and methanogens. Metabolism was substantially accelerated when GAC was added to co-cultures of Geobacter metallireducens and Geobacter sulfurreducens grown under conditions previously shown to require DIET. Cells were attached to GAC, but did not aggregate as they do when making biological electrical connections between cells. …
Electrical Conductivity In A Mixed-Species Biofilm, Nikhil S. Malvankar, Joanne Lau, Kelly Nevin, Ashley E. Franks, Mark T. Tuominen, Derek R. Lovley
Electrical Conductivity In A Mixed-Species Biofilm, Nikhil S. Malvankar, Joanne Lau, Kelly Nevin, Ashley E. Franks, Mark T. Tuominen, Derek R. Lovley
Kelly Nevin
Geobacter sulfurreducens can form electrically conductive biofilms, but the potential for conductivity through mixed species biofilms has not been examined. A current-producing biofilm grown from a wastewater sludge inoculum was highly conductive with low charge transfer resistance even though microorganisms other than Geobacteraceae accounted for nearly half the microbial community.