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Articles 1 - 6 of 6
Full-Text Articles in Life Sciences
Enzymatic Degradation Of Microcystin-Lr By Microcystinase (Mlra), Faisal Alqhtani
Enzymatic Degradation Of Microcystin-Lr By Microcystinase (Mlra), Faisal Alqhtani
Graduate Theses and Dissertations
Microcystin-LR (MC-LR) is affecting the water supply worldwide. Hence, a way to eliminate this toxin is an essential target. In this study, successful cloning of the mlrA gene and producing MlrA enzyme that can degrade the cyclic MC-LR to linearized MC-LR was done. MlrA protein was expressed in Escherichia coli BL-21 (E. coli). Also, enhancing the MlrA yield by adding nickel to LB media was a success in producing more MlrA enzyme from the same volume. Even though the enzyme showed no activity after adding Ni, the enzyme was expressed at a higher yield. Furthermore, it was to investigate adding …
Development And Evaluation Of Models For Hatchery-Mediated Infection Of Neonatal Broiler Chicks, Brittany Danielle Graham
Development And Evaluation Of Models For Hatchery-Mediated Infection Of Neonatal Broiler Chicks, Brittany Danielle Graham
Graduate Theses and Dissertations
Formaldehyde fumigation has been used to control the microbial load in commercial hatch cabinets. The hatch cabinet environment promotes replication and dissemination of both apathogenic and pathogenic microorganisms. As the microbial load increases during the hatching phase, formaldehyde eliminates airborne microorganisms circulating in the hatch cabinet environment. Due to the hazardous properties of formaldehyde, non-toxic alternatives to formaldehyde fumigation to control the microbial bloom in hatch cabinets are needed. The objectives of the present dissertation were to develop challenge models using singular or multiple microorganisms associated with the microbial bloom to simulate contamination that occurs in commercial hatch cabinets. Initially, …
Directed Genome Evolution To Identify Genes For Macrophage Survival By Staphylococcus Agnetis, Sonali Lakshika Anne Lenaduwe Lokuge
Directed Genome Evolution To Identify Genes For Macrophage Survival By Staphylococcus Agnetis, Sonali Lakshika Anne Lenaduwe Lokuge
Graduate Theses and Dissertations
Bacterial Chondronecrosis with Osteomyelitis (BCO) is a debilitating infection that negatively impacts animal welfare and costs the broiler industry billions of dollars annually. We have previously isolated Staphylococcus agnetis 908 from BCO samples obtained from broilers at the University of Arkansas research farm. This isolate can induce BCO lameness at greater than 50% in broilers exposed to the pathogen in drinking water. We found that S. agnetis 908 is capable of surviving and escaping macrophages compared to a closely related cattle isolate,1379. Through Directed Genome Evolution (DGE) we identified that this difference is at least partially associated with an alanine …
The Persistence Of Human Norovirus Surrogate In Leafy Greens Production System, Wenjun Deng
The Persistence Of Human Norovirus Surrogate In Leafy Greens Production System, Wenjun Deng
Graduate Theses and Dissertations
Since mostly consumed raw, foodborne pathogen contamination of leafy greens has led to a large number of foodborne disease outbreaks and illnesses each year in the United States. Human noroviruses (hNoV) are the most common viral pathogen transmitted by leafy greens. In this dissertation, the persistence of the hNoV surrogate Tulane virus (TV) on pre-harvest lettuce and microgreens was investigated. Lettuces are the most studied leafy green model, while previous hNoV research has mainly focused on the post-harvest stage of production. Here, pre-harvest hydroponically grown lettuce were used to determine TV persistence on leafy greens. After inoculation on leaves at …
Pathogenicity Of Staphylococcus Agnetis Associated With Lame Broilers With Osteomyelitis, Sura Faris Salih Zaki
Pathogenicity Of Staphylococcus Agnetis Associated With Lame Broilers With Osteomyelitis, Sura Faris Salih Zaki
Graduate Theses and Dissertations
We developed High Resolution Melt Analysis (HERMA) as a rapid and reliable molecular diagnostic assay for the detection and identification of the main bacterial species recovered from the blood and lesions of bacterial chondronecrosis with osteomyelitis (BCO) from the lame broilers at the University of Arkansas poultry research farm. Also, the present study confirms that raising young birds on suspended wire flooring has been proved to successfully induce lameness attributable to BCO with birds being more susceptible to bacteraemia than those that were raised on litter flooring. The newly described pathogen, Staphylococcus agnetis, has been reported to be overrepresented in …
Developing A Microdialysis Sampling-Based Biofilm/Macrophage Co-Culture Model, Alda Diaz Perez
Developing A Microdialysis Sampling-Based Biofilm/Macrophage Co-Culture Model, Alda Diaz Perez
Graduate Theses and Dissertations
The host immune system and bacterial cells are known to interact during the human lifetime. Bacteria secrete a wide variety of signaling molecules, known as quorum sensing (QSC) molecules, that modulate the host immune system. While immune-biofilm interactions involve this chemical signaling network, the mechanisms through which this occurs are not well understood. This work aimed to develop a new method that can be used not only in vitro settings but also in vivo. The microdialysis sampling technique has widely been used in in vitro and in vivo settings in humans, mice, and rats for the collection of neuropeptides, cytokines, …